Efficacy of Chondroprotective Food Supplements Based on Collagen Hydrolysate and Compounds Isolated from Marine Organisms

Abstract

Osteoarthritis belongs to the most common joint diseases in humans and animals and shows increased incidence in older patients. The bioactivities of collagen hydrolysates, sulfated glucosamine and a special fatty acid enriched dog-food were tested in a dog patient study of 52 dogs as potential therapeutic treatment options in early osteoarthritis. Biophysical, biochemical, cell biological and molecular modeling methods support that these well-defined substances may act as effective nutraceuticals. Importantly, the applied collagen hydrolysates as well as sulfated glucosamine residues from marine organisms were strongly supported by both an animal model and molecular modeling of intermolecular interactions. Molecular modeling of predicted interaction dynamics was evaluated for the receptor proteins MMP-3 and ADAMTS-5. These proteins play a prominent role in the maintenance of cartilage health as well as innate and adapted immunity. Nutraceutical data were generated in a veterinary clinical study focusing on mobility and agility. Specifically, key clinical parameter (MMP-3 and TIMP-1) were obtained from blood probes of German shepherd dogs with early osteoarthritis symptoms fed with collagen hydrolysates. Collagen hydrolysate, a chondroprotective food supplement was examined by high resolution NMR experiments. Molecular modeling simulations were used to further characterize the interaction potency of collagen fragments and glucosamines with protein receptor structures. Potential beneficial effects of collagen hydrolysates, sulfated glycans (i.e., sulfated glucosamine from crabs and mussels) and lipids, especially, eicosapentaenoic acid (extracted from fish oil) on biochemical and physiological processes are discussed here in the context of human and veterinary medicine.

Preparation, Characterization, Evaluation of Neuroprotective Effect, and Related Mechanisms of Phosphatidylserine Emulsion in 5- and 12-Week Old Mice

“…The emulsion is a thermodynamically unstable system, which mainly has problems with physical instability. , Thus, improving emulsion physical stability against gravitational separation and droplet aggregation has attracted much more interest. Beside health benefits in food administration, our results also provide important insights when peptide- and protein-based drugs have to be encapsulated for an oral use in a clinical context. − …”

Evidence for Quantum Chemical Effects in Receptor-Ligand Binding Between Integrin and Collagen Fragments — A Computational Investigation With an Impact on Tissue Repair, Neurooncolgy and Glycobiology

“…ADAM (A Disintegrin And metalloprotease) 12 has also been implicated in the development of pathogen various forms of cancer, hypertension, liver fibrogenesis, and asthma ( Estrella et al, 2009 ). Since collagen–integrin ( Siebert et al, 2010 ) and collagen–ADAM interactions ( Eckert et al, 2021 ) play a dominant role in cell experiments ( Petridis et al, 2011 ; Martinez-Olivera et al, 2018 ) where Mg 2+ is replaced by Co 2+ we have already discussed their feasibility here.…”

“…Our analysis shows that bond cleavage of axial and equatorial ligands can vary within a few kcal/mol in binding energy. The quantum chemical DFT data may contribute to a better understanding of the function relationship of integrin ligand complexes including the role of integrins in tissue repair, glycobiology and neurooncology ( Racine-Samson et al, 1997 ; Gabius et al, 2004 ; André et al, 2005 ; Bhunia et al, 2010 ; Petridis et al, 2011 ; Stötzel et al, 2012 ; Tsvetkov et al, 2012 ; Kanakis et al, 2013 ; Glinskii et al, 2014 ; Hussein et al, 2015 ; Zhang et al, 2016a ; Cornelissen and Van Vliet, 2016 ; Malric et al, 2017 ; Marsico et al, 2018 ; Martinez-Olivera et al, 2018 ; McCarthy et al, 2018 ; Sharma et al, 2020 ; Eckert et al, 2021 ). The polysialylated neural cell adhesion molecule (NCAM) as well as the sulfated HNK-1 epitope are together with β1-integrin involved in cell differentiation processes.…”

“…A detailed understanding of integrin–ligand interactions requires that the impact of ligand structures is considered ( Racine-Samson et al, 1997 ; Gabius et al, 2004 ; Petridis et al, 2011 ; Glinskii et al, 2014 ; Hussein et al, 2015 ; Cornelissen and Van Vliet, 2016 ; Marsico et al, 2018 ; Sharma et al, 2020 ; Eckert et al, 2021 ). The corresponding data are necessary to understand cellular mechanisms in neuroblastoma and in glioblastoma cells ( Hemler, 1990 ; André et al, 2005 ; Bhunia et al, 2010 ; Petridis et al, 2011 ; Stötzel et al, 2012 ; Tsvetkov et al, 2012 ; Kanakis et al, 2013 ; Zhang et al, 2016a ; Malric et al, 2017 ; Martinez-Olivera et al, 2018 ; McCarthy et al, 2018 ; Van Agthoven et al, 2019 ).…”

Potential of Thermolysin-like Protease A69 in Preparation of Bovine Collagen Peptides with Moisture-Retention Ability and Antioxidative Activity

“…Collagen hydrolysates have been effectively utilized due to their good moisturizing properties at the stratum corneum layer of the skin, which reduce the effects of skin dryness, laxity and wrinkles [45]. Eckert et al (2021) reported that collagen hydrolysates of fish, jellyfish and bovine origin that were used as food supplements could maintain cartilage health of dogs and could be as potential therapeutic drugs in early osteoarthritis [46]. Schadow et al (2017) used several collagen hydrolysates as nutraceuticals for human osteoarthritis, and found that there were marked differences between collagen hydrolysates of different origins [47].…”