Efficacy of Contact Lens Storage Solutions against Different Acanthamoeba Strains

Hiti, K.; Walochnik, Julia; Haller-Schober, Eva Maria; Faschinger, Christoph; Aspöck, Horst

doi:10.1097/01.ico.0000214204.22200.7f

Cited by 38 publications

(32 citation statements)

References 33 publications

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“…While the underlying mechanism for this risk remains unknown, none of the widely-used multipurpose solutions have been shown to be highly efficacious against Acanthamoeba; however they have generally been adequate to suppress large AK outbreaks in the past. 8,[30][31][32][33][34][35][36] This suggests the risk presented by use of Complete MoisturePlus may be a relative inability to withstand an Acanthamoeba challenge compared to that of other solutions. Results similarly demonstrate non-statistically significant patterns of risk suggesting hygiene-and potentially water-related factors such as showering with contact lenses may contribute to AK.…”

Section: -58mentioning

confidence: 99%

The Association of Contact Lens Solution Use and Acanthamoeba Keratitis

Joslin

Shoff

et al. 2007

American Journal of Ophthalmology

219

152

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Purpose-Diagnosis of Acanthamoeba keratitis, a rare but serious corneal infection, has recently increased significantly at the University of Illinois at Chicago (UIC) Cornea Service. The purpose is to investigate Acanthamoeba keratitis risk factors.Design-Retrospective case-control study. Results-Thirty-nine (73.6%) cases and 113 (65.3%) controls participated; 38 cases had complete contact lens data. Thirty-five of 38 cases (92.1%) and 47 of 100 controls (47.0%) used soft lenses. Analysis was performed on 30 cases and 39 controls with matched pairs with soft lens use. Exclusive use of AMO Complete MoisturePlus Multi-Purpose Solution was independently associated with Acanthamoeba keratitis in multivariable analysis (55.2% vs. 10.5%; OR, 16.67; 95% CI, 2.11-162.63; p = 0.008). However, 38.8% of cases reported no use of AMO Complete MoisturePlus MultiPurpose Solution or used it in combination with other solutions. Although not statistically significant, additional hygiene-related variables (solution 'reuse', lack of 'rubbing', and showering with lenses) suggest a pattern of risk,. Conclusions-AMO Complete MoisturePlusMulti-Purpose Solution use is independently associated with Acanthamoeba keratitis among soft contact lens users. However, it does not explain all cases, suggesting additional factors. Further research into environmental risk factors and hygiene

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Section: -58mentioning

confidence: 99%

The Association of Contact Lens Solution Use and Acanthamoeba Keratitis

Joslin

Shoff

et al. 2007

American Journal of Ophthalmology

219

152

View full text Add to dashboard Cite

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“…Trophozoites in exponential growth (72 to 96 h) were concentrated by centrifugation at 500×g for 10 min. The amoebas were washed twice in sterile Neff's saline solution (1.2 g NaCl, 0.4 g MgSO 4 ·H 2 O, 0.4 g CaCl 2 ·2H 2 O, 1.42 g Na 2 HPO 4 , and 1.36 g KH PO 4 in 100 ml distilled water), counted in a hemacytometer, adjusted to final concentrations in Neff's saline solution at a density of 10 4 amebae/ml (Hiti et al 2006) (95% trophozoites), and used immediately for testing.…”

Section: Parasites and Culturementioning

confidence: 99%

Efficacy of contact lens storage solutions against trophozoite and cyst of Acanthamoeba castellanii strain 1BU and their cytotoxic potential on corneal cells

2007

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Acanthamoeba is a free-living amoeba causing a potentially blinding infection of the cornea. Contact lens wearers are most at risk and account for about 95% of cases. We investigated the in vitro effectiveness of 10 contact lens solutions against Acanthamoeba castellanii and their cytotoxicity on corneal cells in vitro. Contact lens solutions were evaluated for their amoebicidal activities using an inverted light microscope. To determine of their cytotoxicity on corneal cells, agar diffusion tests were performed. According to the results obtained from the tests, AVIZOR Aqua Soft Comfort and Elegance(R) showed the best amoebicidal effect on A. castellanii trophozoites. Cysts were still viable after overnight (8 h) exposure. In the case of the cyctotoxic acitivities, All In One Light, Astek, SOLO-Care Aqua, Maxima, and Horien showed no cytotoxicity on the corneal cells. ReNu MultiPlus, AVIZOR Aqua Soft Comfort, Carrera, and Elegance showed mild cytotoxicity on the corneal cells. Plurisol.M presented moderate cytotoxicity on the corneal cells. All commercial solutions examined in this study are the lack of efficacy against A. castellanii. Improvement or development of new contact lens disinfecting systems by the manufacturers is needed to prevent Acanthamoeba keratitis.

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“…In Thailand, a study showed that about 5% of cases occur in contact lenses wearers (Preechawat et al 2007). The use of effective contact lens solutions and adequate disinfection times are a fundamental method of preventing corneal infection by Acanthamoeba (Beattie et al 2003;Hiti et al 2006;Kilvington and Anger 2001;Niszl and Markus 1998). The clinical symptoms of AK are similar to those of herpes simplex keratitis and fungal keratitis (Bacon et al 1993;Mathers et al 1996), so this condition can easily be misdiagnosed.…”

Section: Introductionmentioning

confidence: 99%

A simple PCR condition for detection of a single cyst of Acanthamoeba species

2011

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Acanthamoeba is a free-living protozoan with a worldwide distribution in a variety of natural and artificial habitats. It has even been found in contact lens solution. Acanthamoeba spp. can cause infections such as granulomatous amoebic encephalitis and amoebic keratitis. Specific and sensitive diagnosis of Acanthamoeba infections can prevent clinical symptoms from worsening. Recently, PCR technique has been used for Acanthamoeba diagnosis. Unfortunately the dormant cyst of Acanthamoeba is resistant to chemical reagents; thus, most extraction of DNA uses a commercial DNA extraction kit for obtaining DNA for further use in polymerase chain reaction (PCR). Therefore in the present study, we improved the ability to diagnose Acanthamoeba using a simplified PCR technique. Interestingly, heating at 94°C for 10 min could release DNA which is amplified with specific primers designed from 16S rRNA. The PCR product is about 180 bp. This technique is a simple and efficient method for detection of Acanthamoeba-even a single cyst-and does not require high-cost reagents or complicated procedures to extract DNA.

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Efficacy of Contact Lens Storage Solutions against Different Acanthamoeba Strains

Abstract: Two of the tested contact lens solutions (the ones for rigid gas permeable lenses) destroyed the cysts of all strains. One of these, containing a combination of two amoebicidal ingredients even after only one hour of exposure time.

Cited by 38 publications

References 33 publications

The Association of Contact Lens Solution Use and Acanthamoeba Keratitis

The Association of Contact Lens Solution Use and Acanthamoeba Keratitis

Efficacy of contact lens storage solutions against trophozoite and cyst of Acanthamoeba castellanii strain 1BU and their cytotoxic potential on corneal cells

A simple PCR condition for detection of a single cyst of Acanthamoeba species

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