Efficacy of curculigoside in protecting against ischemic brain injury through regulation of oxidative stress and NF-κB and PI3K/Akt expression

Xu, Yan; Wen, Kuo; Liu, Aijun; Wang, Xinna; Xu, Hanying; Wen, Hongjuan

doi:10.1016/j.jep.2022.115804

Cited by 8 publications

(1 citation statement)

References 26 publications

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“…8 31 , 41 , 42 . PI3K pathway also had close cross-talk with the MAPK pathway and contributed to this pro-inflammatory effect 38 , 43 . Many studies had found the effect of VEGF and the production of inflammatory factors were dependent on these two pathways, and the main components GA and AS could inhibit this process, thus SQWMG might regulate dysregulated angiogenesis and vascular inflammation by inhibition of these pathways, and eventually remarkably decrease of gene expression of inflammatory factors.…”

Section: Discussionmentioning

confidence: 99%

Exploring the components and mechanisms of Shen-qi-wang-mo granule in the treatment of retinal vein occlusion by UPLC-Triple TOF MS/MS and network pharmacology

Zhao

Qiu

et al. 2023

Sci Rep

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This study aimed to explore the substance basis and mechanisms of Shen-qi-wang-mo Granule (SQWMG), a traditional Chinese medicine prescription that had been clinically utilized to treat retinal vein occlusion (RVO) for 38 years. Components in SQWMG were analyzed by UPLC-Triple-TOF/MS and a total of 63 components were identified with ganoderic acids (GA) being the largest proportion. Potential targets of active components were retrieved from SwissTargetPrediction. RVO-related targets were acquired from related disease databases. Core targets of SQWMG against RVO were acquired by overlapping the above targets. The 66 components (including 5 isomers) and 169 targets were obtained and concluded into a component-target network. Together with biological enrichment analysis of targets, it revealed the crucial role of the “PI3K-Akt signaling pathway”, “MAPK signaling pathway” and their downstream factor iNOS and TNF-α. The 20 key targets of SQWMG in treating RVO were acquired from the network and pathway analysis. The effects of SQWMG on targets and pathways were validated by molecular docking based on AutoDock Vina and qPCR experiment. The molecular docking showed great affinity for these components and targets, especially on ganoderic acids (GA) and alisols (AS), which were both triterpenoids and qPCR exhibited remarkably reduced inflammatory factor gene expression through regulation of these two pathways. Finally, the key components were also identified from rat serum after treatment of SQWMG.

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