Efficacy of denture cleansers on denture liners contaminated with Candida species

Abstract

As poor denture hygiene is related to Candida colonisation, disinfectant solutions have been proposed as an effective method of preventing denture stomatitis. This study assessed the efficacy of denture cleansers on Candida albicans and Candida glabrata adherence on denture liners. Another aim was to correlate materials' surface roughness (Ra) to Candida adherence. Specimens of three denture liners (soft and hard polymethyl methacrylate (PMMA)-based and soft silicone-based) were prepared and had their Ra measured. Specimens were randomly divided to adherence assays with C. albicans or C. glabrata. After contamination with the fungi, specimens were treated with an enzymatic cleanser solution, a cleanser solution or a 0.5% NaOCl solution by soaking for 3, 15 or 10 min, respectively. Control group specimens were soaked in distilled water for 15 min. Number of remaining Candida cells after treatment was determined by light microscopy (x400). Analysis of variance (alpha = 0.05) showed that Ra of the silicone-based liner was lower than that of the PMMA-based liners (p < 0.05). The overall results showed high C. glabrata adherence (p < 0.001), while the lowest levels of remaining Candida cells were found for the treatment with 0.5% NaOCl (p = 0.0019). No difference among denture cleansers and control was found (p = 0.19). There was no correlation between Ra and C. albicans or C. glabrata adherence in all materials tested. The only treatment able to reduce both Candida species adherence on all materials tested was 0.5% NaOCl solution.

Effect of incorporation of surface pre-reacted glass ionomer filler in tissue conditioner on the inhibition of <i>Candida albicans</i> adhesion

“…As alternative cleaning methods, chemical cleaning such as soaking in sodium hypochlorite 8) or denture cleansers 9) are known to be effective. Sodium hypochlorite is commonly used as a disinfectant as it is highly bactericidal and fungicidal 10) ; however, it decolorizes the denture materials and corrodes the metals 9) .…”

“…Sodium hypochlorite is commonly used as a disinfectant as it is highly bactericidal and fungicidal 10) ; however, it decolorizes the denture materials and corrodes the metals 9) . Immersion in denture cleanser is more effective than washing the TC with water 11) .…”

Effect of Denture Cleansers on Flexural Strength, Color Stability and Surface Roughness of Flexible and Microwave cured Acrylic Resin

“…The literature has shown that sodium hypochlorite and alkaline peroxides are the two main classes of immersion denture cleansers 27 . Sodium hypochlorite is inexpensive, presents bactericidal and fungicidal action, as well as stain removal properties, requires a short period of disinfection, and have been suggested as effective denture cleansers [28][29][30] . Moreover, alkaline hypochlorite cleansers contain 5% sodium hypochlorite act directly on organic matrix of plaque causing dissolution of polymer structure.…”

Influence of cleansers and a phytotherapic against Candida albicans on the properties of denture liners

“…However, NaOCl was used in a low concentration (0.5%), which seems to present no detrimental effect on resilient denture liners. 15,16 This result is important since this concentration may result in adequate control of biofilm formation without alteration the original characteristics of the denture liners. However, it is worth saying that in this study, the effects on the assessed properties of resilient liners were tested only for 14 days and this may have influenced the results, especially considering that other liners may be used for longer periods.…”