Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

Satpati, Gour Gopal; Gorain, Prakash Chandra; Pal, Ruma

doi:10.1155/2016/8712470

Cited by 27 publications

(13 citation statements)

References 42 publications

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“…Table 4 summarizes the effect of nitrogen availability and starvation conditions on biomass yield, lipid content and fatty acids in algae. It is evident from Table 4 that the fatty acids profile in microalgae cultivated in phosphorus-replete and -deplete conditions are predominantly of C16:1 and C18:1 [4,6,32,41,49,[54][55][56][57].…”

Section: Role Of Phosphorusmentioning

confidence: 99%

Influence of Nitrogen and Phosphorus on Microalgal Growth, Biomass, Lipid, and Fatty Acid Production: An Overview

Yaakob

Mohamed

Gokare

et al. 2021

Cells

348

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Microalgae can be used as a source of alternative food, animal feed, biofuel, fertilizer, cosmetics, nutraceuticals and for pharmaceutical purposes. The extraction of organic constituents from microalgae cultivated in the different nutrient compositions is influenced by microalgal growth rates, biomass yield and nutritional content in terms of lipid and fatty acid production. In this context, nutrient composition plays an important role in microalgae cultivation, and depletion and excessive sources of this nutrient might affect the quality of biomass. Investigation on the role of nitrogen and phosphorus, which are crucial for the growth of algae, has been addressed. However, there are challenges for enhancing nutrient utilization efficiently for large scale microalgae cultivation. Hence, this study aims to highlight the level of nitrogen and phosphorus required for microalgae cultivation and focuses on the benefits of nitrogen and phosphorus for increasing biomass productivity of microalgae for improved lipid and fatty acid quantities. Furthermore, the suitable extraction methods that can be used to utilize lipid and fatty acids from microalgae for biofuel have also been reviewed.

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Section: Role Of Phosphorusmentioning

confidence: 99%

Influence of Nitrogen and Phosphorus on Microalgal Growth, Biomass, Lipid, and Fatty Acid Production: An Overview

Yaakob

Mohamed

Gokare

et al. 2021

Cells

348

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“…and Chlorococcum sp. respectively, under phosphorus starvation 21 . Phosphorus at 2.7 mg/l gave higher biomass and removal efficiencies, showed a slight (9.5%) increase in lipid content when compared to control.…”

Section: Effect Of Phosphorus Concentration On Lipid Productivity Andmentioning

confidence: 99%

Growth, Lipid Productivity and Cellular Mechanism of Lipid Accumulation in Microalgae <i>Monoraphidium</i> sp. Following Different Phosphorous Concentrations for Biofuel Production

Dhup¹,

Kannan²,

Dhawan³

2017

Current Science

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Stress to an algal species can be caused by several factors ranging from temperature extremes to varying light intensities under which they accumulate lipids. Stress caused by nutrient limitation is known to induce maximum lipid accumulation in microalgae. In order to obtain an appropriate phosphate concentration exhibiting both high biomass and high lipid contents, four different concentrations of phosphate were implemented. Mechanism of lipid accumulation was also studied. Of the tested concentrations, no significant difference between growth rates was observed. Cultures with phosphate concentration 2.7 mg/l exhibited maximum increase in biomass productivity compared to control. But phosphate concentration 0.5 mg/l demonstrated the highest lipid productivity (8.75 mg/l/day), in accordance with lipid content (21.8%). Further, representation of TEM and fluorescent microscopic images depicted differences in lipid accumulation and sub-cellular ultrastructure at different concentrations of phosphate. Disappearance of phosphate and nitrate from the medium was also evaluated to study the efficient nutrient concentration and to better understand mechanism of lipid enhancement.

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“…EDTA has also been reported to improve biomass and pigment production of Chlorococcum sp. by Satpadi et al (43) . Since, iron is indirectly involved in pigment biosynthesis, improvement of iron uptake might have also contributed to zeaxanthin accumulation of Cyanophycean microalgae T. thiebautii.…”

Section: Optimization Of Nutrient Concentration Using Plackett -Burmamentioning

confidence: 91%

Statistical optimization of culture medium composition for enhanced zeaxanthin production by Cyanophycean microalgae Trichodesmium thiebautii (NIOT 152)

Srinivasan¹

2020

IJST

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Background/Objectives: Zeaxanthin is a xanthophyll carotenoid revered for its role in the prevention of age related macular degeneration. The study evaluated the zeaxanthin accumulation of the marine Cyanophycean alga Trichodesmium thiebautii (NIOT 152). A sequential statistical technique was applied to optimize the Artificial Sea Water nutrient medium (ASN-III) components for enhancing the zeaxanthin accumulation in T. thiebautii. Methods: A two-level statistical approach involving Plackett-Burman (PB) design to screen the most important nutrients influencing the zeaxanthin accumulation followed by Response surface methodology (RSM) was employed. The results of PB design revealed sodium nitrate, disodium EDTA, magnesium sulphate and sodium carbonate as the crucial medium components for increasing zeaxanthin accumulation. Further, RSM was employed to study the interaction between these factors and identified an optimum concentration of the ingredients for higher zeaxanthin production. Findings: The optimized medium components resulted in 2.33 fold increase in zeaxanthin accumulation (4.3 ± 1.29 mg L-1) as compared to ASN III medium (1.84 ± 0.12 mg L-1). Novelty: There are only few studies on laboratory cultured Trichodesmium and only very few reports are available regarding pigment production from Trichodesmium sp. The present study successfully demonstrated the statistical optimization of ASN III medium to improve zeaxanthin accumulation by Trichodesmium thiebautii. Keywords: ASN III medium; zeaxanthin; Trichodesmium thiebautii; Plackett-Burman; response surface methodology REFERENCE

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Efficacy of EDTA and Phosphorous on Biomass Yield and Total Lipid Accumulation in Two Green Microalgae with Special Emphasis on Neutral Lipid Detection by Flow Cytometry

Cited by 27 publications

References 42 publications

Influence of Nitrogen and Phosphorus on Microalgal Growth, Biomass, Lipid, and Fatty Acid Production: An Overview

Influence of Nitrogen and Phosphorus on Microalgal Growth, Biomass, Lipid, and Fatty Acid Production: An Overview

Growth, Lipid Productivity and Cellular Mechanism of Lipid Accumulation in Microalgae <i>Monoraphidium</i> sp. Following Different Phosphorous Concentrations for Biofuel Production

Statistical optimization of culture medium composition for enhanced zeaxanthin production by Cyanophycean microalgae Trichodesmium thiebautii (NIOT 152)

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