Efficacy of egg surface disinfectants in captive spawning Atlantic cod Gadus morhua L. and haddock Melanogrammus aeglefinus L.

Abstract

In an e¡ort to optimize the e⁄ciency of high-density incubation of Atlantic cod Gadus morhua and haddock Melanogrammus aegle¢nus eggs, the per cent hatch of eggs treated with four disinfectants (3% hydrogen peroxide, 1% polyvinylpyrrolidone iodine, 0.1% sodium hypochlorite and a 0.005% antibiotic solution^penicillin/streptomycin) was compared in both species. The per cent hatch of eggs of each species was greatest after a 24 h treatment with the antibiotic solution. The hatching success of eggs treated within the di¡erent disinfectant treatments depended upon the embryonic developmental stage in both species. The sodium hypochlorite treatment had the lowest % coverage of colony growth after disinfected haddock eggs were plated onto sterile agar media, highest survival rates to the end of the embryonic period, but the lowest per cent hatch.

Estimating growth in haddock larvae Melanogrammus aeglefinus from RNA:DNA ratios and water temperature

“…) and penicillin (17.5 mg l -1 ) were added to each tank to control bacterial growth (Peck et al 2004). Tanks were gently aerated and the water was not exchanged until 50% hatch was observed 12, 11, and 9 d after fertilization, at 6, 8, and 10°C, respectively.…”

Effect of iodine treatment on the hatching rate of black sea turbot, (Psetta maxima Linnaeus, 1758), eggs

“…According to Barker et al (1989) and Sauter et al (1987), bacteria can influence egg survival. For this reason, disinfection of eggs has been widely used to reduce egg mortality and improve rearing success during the yolk sac and first feeding stages (Salvesen et al, 1997;Harboe et al, 1994;Bergh and Jelmert, 1996;Peck et al, 2004). A number of methods have been developed to disinfect fish eggs.…”

Penicillin-G: Efficacy against <i>Flavobacterium psychrophilum</i> and evaluation of lethal dose limits for rainbow trout

“…For example, Oppenheimer [28] significantly improved hatching success using penicillin G and streptomycin (50 mg/L of each) to treat eggs of Sardinops, Pleuronichthys and Gadus; Aureomycin and polymyxin combined also gave good results. Similarly Peck et al [29] found that 24 h treatment of Atlantic cod Gadus morhua or haddock Melanogrammus aeglefinus eggs with a 0.005% solution of penicillin-streptomycin improved hatching success. Arenzon et al [30] 1 h), or penicillin-G (50 IU/mL for 1 h) did not completely eliminate R. salmoninarum from Atlantic salmon Salmo salar eggs [32].…”