Efficacy of egg yolk based and egg yolk free soya bean milk based extenders for cryopreservation of Zebu cattle and buffalo semen

Abstract

This study was undertaken on three mature bulls each of Gir cattle and Surti buffalo breeds to evaluate the comparative efficacy of egg yolk based standard TFYG (Tris-citrate-fructose-yolk-glycerol) extender and egg yolk free soybean based commercial extenders Optixcell® (IMV, France) and Andromed® (Minitube, Germany) under split-sample technique. The ejaculates (9/bull) were extended @ 100×10 6 sperm ml -1 with three extenders and frozen using biofreezer following 4 hr of equilibration. The pooled means of progressively motile sperm observed (irrespective of extenders) at initial, pre-freeze and post-thaw stage in Gir bulls semen were 76.53±0. Optixcell was proved superior, and at par with TFYG, than the Andromed in maintaining greater motility, viability, morphology, acrosomal/plasma membrane integrity including post-thaw sperm longevity of cattle and buffalo spermatozoa with significant differences only in sperm motility and post-thaw longevity. The motile, live and HOST reactive sperm were significantly higher in buffalo semen than cattle at initial and pre-freeze stage, but not at post-thaw stage. The results showed that egg yolk free commercial Optixcell extender and egg yolk based TFYG extender were at par in terms of most of the sperm quality traits, hence any one of them can be preferred over Andromed for successful routine cryopreservation of cattle and buffalo semen.

variables of cryopreserved rabbit spermatozoa as affected by nano-curcumin supplementation into Tris-extender containing egg yolk or soybean lecithin extender

“…The absence of significant differences between TEY and TSBL in all rabbit sperm parameters studied post dilution, equilibration, and thawing may be due to good quality of the composition of both semen extender according to Samad (1985) and Chaudhari and Mshelia (2002), who stated that semen extender must be contained sources of energy (glucose in both), buffering capacity (Tris in both), protectant against cold shock (egg yolk in TEY and SBL in TSBL), cryoprotectant (glycerol in both), and antibiotics (Lincomycin and streptomycin in both). The lecithin in soybean or lecithin and lipoprotein contents in egg yolk together contribute to the preservation of the lipoprotein sheath of the sperm cell (Kumar et al, 1992).…”

Evaluation of Cryoprotective, Cryocapacitation Inhibitory and Fertility Enhancing Potential of RU-486, Sericin and Taurine in Cattle and Buffalo Semen

“…The present findings in control TFYG extender during cryopreservation of semen concurred well with the previous reports of Dhami et al (1994Dhami et al ( , 1995 and Patel et al (2020) in cattle and buffalo semen cryopreserved with same extender. Chaudhary et al (2018) reported comparable high HOS reactive sperm on dilution (77-82%), but lower values in post-thaw semen (28% each) of both Gir and Surti buffalo semen. Zodinsanga et al (2015) observed 93 and 80% postthaw HOS reactive sperm in purebred and crossbred bulls, respectively.…”