Efficacy of essential oil of Syzygium aromaticum alone and in combination with benznidazole on murine oral infection with Trypanosoma cruzi IV

Zanusso, Gerson; Massago, Miyoko; Kian, Danielle; Toledo, Max Jean de Ornelas

doi:10.1016/j.exppara.2018.01.002

Cited by 12 publications

(8 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Currently, only two drugs are available for CD treatment: benznidazole (BZ) and nifurtimox (NFX). Unfortunately, these drugs carry high toxicity and cause severe side effects that often interrupt treatment [43]. Therefore, there is a need for new therapeutic alternatives for CD that are more effective and less toxic for both acute and chronic phases.…”

Section: Introductionmentioning

confidence: 99%

“…In addition, the animals treated with BZ and BZ + Sa EO presented lower patency periods compared with animals treated only with Sa EO, as well as lower positivity of blood cultures compared with the untreated control group. With respect to cure rates, Sa EO showed 12.5% (1/8), BZ showed 25.0% (1/4), and BZ + Sa EO showed 37.5% (3/8), considered a very interesting result [43].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Luna

Scotti

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

The termneglected diseasesrefers to a group of infections caused by various classes of pathogens, including protozoa, viruses, bacteria, and helminths, most often affecting impoverished populations without adequate sanitation living in close contact with infectious vectors and domestic animals. The fact that these diseases were historically not considered priorities for pharmaceutical companies made the available treatments options obsolete, precarious, outdated, and in some cases nonexistent. The use of plants for medicinal, religious, and cosmetic purposes has a history dating back to the emergence of humanity. One of the principal fractions of chemical substances found in plants are essential oils (EOs). EOs consist of a mixture of volatile and hydrophobic secondary metabolites with marked odors, composed primarily of terpenes and phenylpropanoids. They have great commercial value and were widely used in traditional medicine, by phytotherapy practitioners, and in public health services for the treatment of several conditions, including neglected diseases. In addition to the recognized cytoprotective and antioxidative activities of many of these compounds, larvicidal, insecticidal, and antiparasitic activities have been associated with the induction of oxidative stress in parasites, increasing levels of nitric oxide in the infected host, reducing parasite resistance to reactive oxygen species, and increasing lipid peroxidation, ultimately leading to serious damage to cell membranes. The hydrophobicity of these compounds also allows them to cross the membranes of parasites as well as the blood-brain barrier, collaborating in combat at the second stage of several of these infections. Based on these considerations, the aim of this review was to present an update of the potential of EOs, their fractions, and their chemical constituents, against some neglected diseases, including American and African trypanosomiasis, leishmaniasis, and arboviruses, specially dengue.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Luna

Scotti

et al. 2019

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

show abstract

“…In order to evaluate the efficacy of the different treatments, an animal that presented negative results for all tests (FBE, BC, cPCR and qPCR) before and after immunosuppression with Cy, was considered cured [21,28]. An animal that presented a positive result in any of these tests was considered as therapeutic failure.…”

Section: Cure Criteriamentioning

confidence: 99%

“…The EOs of S. aromaticum and of Z. officinale, administered alone, have previously presented anti-T cruzi activity greater than that of BZ in mice orally inoculated with metacyclic trypomastigotes obtained from a culture of Y strain (TcII) [21]. The treatment using the combination of BZ and S. aromaticum EO in mice orally inoculated with metacyclic trypomastigotes of the AM14 strain (TcIV) obtained from the insect vector, also promoted benefits for the treated animals [28]. Together, these results justify the continuation of studies regarding EOs, alone or in combination, using trypomastigotes of different origins (blood, insect or culture) and other strains of T. cruzi in the infection of the animals.…”

Section: Introductionmentioning

confidence: 99%

Essential oils from Syzygium aromaticum and Zingiber officinale, administered alone or in combination with benznidazole, reduce the parasite load in mice orally inoculated with Trypanosoma cruzi II

Sarto

Silva

Fernandes

et al. 2021

BMC Complement Med Ther

Self Cite

View full text Add to dashboard Cite

Background Trypanosoma cruzi is the etiological agent of Chagas disease (CD) or American trypanosomiasis, an important public health problem in Latin America. Benznidazole (BZ), a drug available for its treatment, has limited efficacy and significant side effects. Essential oils (EOs) have demonstrated trypanocidal activity and may constitute a therapeutic alternative. Our aim was to evaluate the efficacy of the EOs of clove (CEO - Syzygium aromaticum) and ginger (GEO - Zingiber officinale), administered alone and in combination with BZ, in Swiss mice infected with T. cruzi. Methods The animals were inoculated with 10,000 blood trypomastigotes of the Y strain of T. cruzi II by gavage and divided into four groups (n = 12 to 15): 1) untreated control (NT); 2) treated with BZ; 3) treated with CEO or GEO; and 4) treated with BZ + CEO or GEO. The treatments consisted of oral administration of 100 mg/kg/day, from the 5th day after parasite inoculation, for 20 consecutive days. All groups were submitted to fresh blood examination (FBE), blood culture (BC), conventional PCR (cPCR) and real-time PCR (qPCR), before and after immunosuppression with cyclophosphamide. Results Clove and ginger EOs, administered alone and in combination with BZ, promoted suppression of parasitemia (p < 0.0001), except for the animals treated with CEO alone, which presented a parasitemia curve similar to NT animals. However, there was a decrease in the BC positivity rate (p < 0.05) and parasite load (< 0.0001) in this group. Treatment with GEO alone, on the other hand, besides promoting a decrease in the BC positivity rate (p < 0.05) and parasite load (p < 0.01), this EO also resulted in a decrease in mortality rate (p < 0.05) of treated mice. Conclusions Decreased parasite load, as detected by qPCR, was observed in all treatment groups (BZ, CEO, GEO and BZ + EOs), demonstrating benefits even in the absence of parasitological cure, thus opening perspectives for further studies.

show abstract

“…Zanusso Júnior et al (2018) [ 39 ] investigated the activity of the EO of Syzygium aromaticum (main compounds are eugenol and β-caryophyllene) alone and in association with benznidazole (BZ) in mice infected with T. cruzi AM14 strain (TcIV) (considered resistant to BZ in vivo ). When compared to untreated animals, experiments with the EO of S. aromaticum alone promoted a reduction of the parasitemia.…”

Section: Essential Oils With Trypanocidal Activitymentioning

confidence: 99%

Trypanocidal Essential Oils: A Review

et al. 2020

View full text Add to dashboard Cite

Trypanosomiases are diseases caused by parasitic protozoan trypanosomes of the genus Trypanosoma. In humans, this includes Chagas disease and African trypanosomiasis. There are few therapeutic options, and there is low efficacy to clinical treatment. Therefore, the search for new drugs for the trypanosomiasis is urgent. This review describes studies of the trypanocidal properties of essential oils, an important group of natural products widely found in several tropical countries. Seventy-seven plants were selected from literature for the trypanocidal activity of their essential oils. The main chemical constituents and mechanisms of action are also discussed. In vitro and in vivo experimental data show the therapeutic potential of these natural products for the treatment of infections caused by species of Trypanosoma.

show abstract

Efficacy of essential oil of Syzygium aromaticum alone and in combination with benznidazole on murine oral infection with Trypanosoma cruzi IV

Cited by 12 publications

References 39 publications

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Active Essential Oils and Their Components in Use against Neglected Diseases and Arboviruses

Essential oils from Syzygium aromaticum and Zingiber officinale, administered alone or in combination with benznidazole, reduce the parasite load in mice orally inoculated with Trypanosoma cruzi II

Trypanocidal Essential Oils: A Review

Contact Info

Product

Resources

About