I n Pakistan Red Sindhi and Sahiwal are valuable milk breeds of global recognition, due to their distinctive characteristics. In addition to these well-known breeds of cattle, there is a large number of non-expressive and crossbred cattle in Pakistan. Among them, Achai, is a short stature, reddish brown coated breed of Khyber Abstract | This study evaluated the efficiency of two different estrus synchronization protocols in Achai breed of cattle. Twelve lactating Achai cows were divided into two equal groups and subjected to Presynch Ovsynch (POP) or Modified 7-day Select Synch (MSS) protocol. In POP protocol animals were administered with PGF 2 -α injections on day -38, -24 and -3. GnRH-1 was given on day -10 and 0, along with timed artificial insemination after 16 hrs. Animals in MSS group were administered with a controlled internal drug release (CIDR) insert on day -7 with simultaneous use of prostaglandin 2-alpha (PGF 2 -α), GnRH-1 at day -5, removal of CIDR at day 0, concurrent with two doses of PGF 2 -α at 8 hr. interval, GnRH-2 on day 3, followed by TAI after 16 hrs. Results showed higher estrogen (E 2 ) and lower progesterone (P 4 ) concentrations for MSS than POP group. Cows with higher E 2 and lower P 4 levels at estrus got conceived (P<0.05). Highest level of E 2 was recorded in pregnant cows in POP group in comparison with MSS. The P 4 concentration of pregnant cows in MSS was lowest at the time of insemination (P<0.05). Induction of fertile estrous was maximum in POP group than MSS, but conception rate in cows receiving TAI was significantly higher in MSS than POP group (66.66% vs 33.33%;). Efficiency of MSS protocol was virtually double than POP for estrus synchronization and conception in Achai cattle.