Efficacy of evaluation of rooster sperm morphology using different staining methods

Łukaszewicz, Ewa; Jerysz, Anna; Partyka, Agnieszka; Siudzińska, Anna

doi:10.1016/j.rvsc.2008.03.010

Cited by 69 publications

(57 citation statements)

References 23 publications

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“…The highest occurrence of morphological malformations was observed in tails (20.3% in RIR, 28.1% in SU, and 29.7% in BPR). Lukaszewicz et al (2008) reported the highest percentage of morphological defects in heads of spermatozoa of cocks of the laying breed Italian Partridge; depending on method of dyeing, these values ranged from 11.0 to 14.3%. When using the dyeing method published by Blom (1981), Lukaszewicz et al (2008) observed a high number of head deformations (76.1%).…”

Section: Resultsmentioning

confidence: 99%

“…Lukaszewicz et al (2008) reported the highest percentage of morphological defects in heads of spermatozoa of cocks of the laying breed Italian Partridge; depending on method of dyeing, these values ranged from 11.0 to 14.3%. When using the dyeing method published by Blom (1981), Lukaszewicz et al (2008) observed a high number of head deformations (76.1%). The highest and the lowest numbers of spermatozoa with defect heads were found in cocks of the lines RIR and SU (3.2%, P ≤ 0.01 vs. 1.3%, P ≤ 0.05, respectively).…”

Section: Resultsmentioning

confidence: 99%

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Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines

Jarinkovičová¹,

Máchal²,

Máchal³

et al. 2012

Czech J. Anim. Sci.

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In domestic fowl (Gallus gallus f. domestica), monitoring of both quantitative and qualitative parameters of ejaculates is an indispensable precondition of successful artificial insemination; on the basis of the obtained results it is then possible to select the individuals that produce sufficient amounts AbstRAct: Ejaculates and blood plasma were sampled from cocks of three laying lines: Barred Plymouth Rock (BPR), Sussex Light (SU), and Rhode Island Red (RIR). Ejaculates and blood plasma were sampled four times during the laying period of hens. The following ejaculate parameters were determined: sperm motility, concentrations of sperm cells, ejaculate volume. Sperm morphology was examined. In the blood samples, concentrations of glucose, cholesterol, creatinine, calcium, phosphorus, and magnesium were analysed. The motility of spermatozoa of the cocks was 47.0% (BPR), 47.7% (RIR), and 48.3% (SU), respectively. The highest volume of ejaculate was found in BPR line (0.66 cm 3 ), the lowest one in SU line (0.46 cm 3 , P ≤ 0.01); the highest sperm concentration was in SU line (2.46 × 10 6 /mm 3 ), the lowest one in RIR line (1.96 × 10 6 /mm 3 , P ≤ 0.01). The number of morphologically abnormal sperm cells was similar in all lines − 47.0% BPR, 47.7% RIR, and 48.3% SU, respectively. In general, the occurrence of defective spermatozoa was high in all three lines; the most frequent were tail defects (from 20.3 to 29.7%), while sperm cells with developmental anomalies were less frequent (only 0.3 to 0.4%). Calculated phenotypic correlation between sperm motility on the one hand and the occurrence of defective sperm cells on the other was negative (r p = −0.28, P ≤ 0.01), as well as the correlation between sperm motility and sperm numbers, and between sperm motility and ejaculate volume (r p = −0.28, P ≤ 0.01 and r p = −0.31, P ≤ 0.01, respectively). Negative correlations were found between the level of magnesium in blood plasma and numbers of morphologically defective spermatozoa in the ejaculate, defective heads and defective connecting pieces (r p = −0.33, P ≤ 0.01; r p = −0.23, P ≤ 0.05; and r p = −0.26, P ≤ 0.05). Level of magnesium was positively correlated to sperm motility (r p = 0.26, P ≤ 0.05). However, positive correlations existed between concentration of glucose in blood plasma of cocks and numbers of morphologically defective spermatozoa in sampled ejaculates (r p = 0.27, P ≤ 0.01). Our finding is in accordance with the results found in mammals and other animals, and it shows an important role of magnesium as a key contributor to the quality of ejaculate in aviary species, in our case in laying lines of domestic fowl (Gallus domesticus).

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines

Jarinkovičová¹,

Máchal²,

Máchal³

et al. 2012

Czech J. Anim. Sci.

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“…This is the first morphological evidence of any form of head (head-base bending) or neck (disjointed sperm) "bending" taking place in the avian testis. Despite numerous publications on "bent" or "crooked-necked" sperm [2,4,[9][10][11][12][13][14][15][16][17][18][19]23,26] in birds, no information on the origin of this defect has been presented. Both defects could conceivably also develop in the ductus deferens due to a combination of inherent and external factors.…”

Section: Discussionmentioning

confidence: 99%

“…This anomaly was originally described by Saeki [13] as crooked-necked sperm, the term being defined by Maeda et al [16] as an acute bend in the neck region or anywhere along the length of the midpiece. Various terms such as "broken-necked", "bent-neck" and "neck-bending" have also been used and this defect has been described, but not always defined, in a number of avian species including the fowl, duck, goose, turkey, partridge, Japanese quail and parrots [15,17,[19][20][21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Head-base bending and disjointed spermatozoa in the emu (Dromaius novaehollandiae): A morphological comparison of two closely related defects

Plessis

Soley

2011

Theriogenology

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The accurate assessment of avian sperm abnormalities is hampered by a lack of descriptive data and by the confusing terminology currently in use. Critical appraisal of semen samples from the distal ductus deferens of the emu revealed that two closely related yet separate (distinct) defects previously collectively referred to as "bent sperm" or "crooked-necked sperm" could be identified by light and electron microscopy. Head-base bending typically involved a 180° bend at the base of the nucleus which placed the head and midpiece into close apposition and parallel to each other. No part of the neck or midpiece was involved and bending was restricted exclusively to the base of the nucleus. Incomplete chromatin condensation was always associated with the bend. Disjointed sperm, which superficially resembled "bent" sperm, showed complete separation of the neck from 2 the head-base at the level of the connecting piece. All structural elements of the neck region appeared normal. In both defects the region of contact between the head (nucleus) and the neck/midpiece was enclosed as a unit by the plasmalemma. Both defects were observed to originate in the testis; however, their subsequent expression in the ductus deferens cannot be ruled out. These results confirm that head-base bending of emu sperm represents a head defect, whereas disjointed sperm should be classified as a tail (neck/midpiece) defect.

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“…The progressive motility percentage was assessed according to Tabatabaei et al (2009). Sperm livability percentage was determined using eosin/nigrosin stain according to Lukaszewicz et al (2008). Sperm abnormalities acrosome integrity was determined according to Al-Daraji (2001).…”

Section: Semen Evaluationmentioning

confidence: 99%

Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

Shamiah¹,

El-Karim

Eshera³

2017

Journal of Sustainable Agricultural Sciences

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IntroductionThe increasing use of artificial insemination (AI) in poultry industry emphasizes the need for good quality sperm distribution. A proper evaluation of semen prior to AI or storage is very important to avoid losses in fertility. Because semen evaluation is extremely important for semen storage and AI programs, a rapid, economical, objective and fertility predictive method of semen evaluation would be beneficial for the poultry industry protocols. Determination of antioxidant enzymes concentration in semen has been practiced for a long time (Peters, 2008).Lipids are a basic component of semen, contributing to the membrane structure of spermatozoa, the metabolism of the sperm cells, and their ability to capacitate and fertilize the female gamete. In birds, the lipid composition of spermatozoa has an influence on fertility (Ansah and Buckland, 1982). El-Saadany (2002) reported that increasing storage time of diluted semen from local chicken breed for 0, 24, 48 and 72 hr decreased significantly (P<0.01) the fertility rate (FR) by 5.12, 9.55 and 12.53%, respectively. Shamiah et al. (2017) reported that GSH supplementation in semen extender significantly (P<0.05) increased FR. Semen supplemented with 0.2 mM GSH (74.5%) showed higher FR than those supplemented with 0.6 mM GSH or free semen (59.9 and 59.2%, respectively).Sperm cells have a high content of unsaturated T HIS STUDY was carried out to evaluate the effect of glutathione (GSH) supplementation at levels of 0.0 (control), 0.2, 0.4 and 0.6 mM to diluted semen stored for 0, 24, 48 and 72 hr at 5°C, on antioxidant enzymes activities in seminal plasma, semen characteristics and fertility rate (FR) of cocks Inshas strain. Supplementation of 0.6 mM GSH to the diluted cocks semen significantly increased (P<0.05) the concentration of alkaline phosphatase (ALP) (40.2 u/ml) compared to other treatments (33.0, 27.8 and 28.3 u/ml). Superoxide dismutase (SOD) concentration in seminal plasma was not significantly affected (P<0.05) by different levels of GSH, storage period or interaction between levels of GSH and storage period. Glutathione peroxidase (GSH-Px) concentration in seminal plasma was not affected by different levels of GSH (P<0.05). Total antioxidant capacity (TACO) in seminal plasma was significantly affected (P<0.05) by different levels of GSH, storage period or interaction between them. There was a positive correlation (r=0.089 and r = 0.168) between SOD concentration and (GSH-Px and TAOC) concentration, but negative correlation (r=-0.119) with ALP concentration. Positive insignificant correlation between (ALP, SOD and TACO) concentration in seminal plasma with the progressive motility, live sperm, curled tail and fertility, while positive significant correlation between GSH-Px concentration with progressive motility, curled tail and fertility. It could be concluded that the addition of GSH to semen extender of cockerels especially at levels 0.2 mM improved the antioxidant enzymes activities, which play a vital role in lowering the deleterious effe...

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Efficacy of evaluation of rooster sperm morphology using different staining methods

Cited by 69 publications

References 23 publications

Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines

Relationship of ejaculate quality and selected biochemical parameters of blood in cockerels of three laying lines

Head-base bending and disjointed spermatozoa in the emu (Dromaius novaehollandiae): A morphological comparison of two closely related defects

Antioxidant enzymes activity and its relation with sperm characteristics and fertility of local cocks as affected by glutathione levels and storage period

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