Efficacy of fixed duration multidrug therapy for the treatment of multibacillary leprosy: A prospective observational study from Northern India

Abstract

Background: In endemic regions of several countries, the prevalence of leprosy has not come down to the level of elimination. On the contrary, new cases are being detected in large numbers. Clinically, it is frequently noted that despite completion of multibacillary multidrug therapy for 12 months, the lesions remain active, especially in cases with high bacteriological indices. Aim: The present study focused on finding out the viable number of Mycobacterium leprae during the 12-month regimen of multibacillary multidrug therapy, at six and 12 months intervals and, attempting to determine their role in disease transmission. Methods: Seventy eight cases of multibacillary leprosy cases were recruited from leprosy patients registered at The Leprosy Mission hospitals at Shahdara (Delhi), Naini (Uttar Pradesh) and Champa (Chhattisgarh), respectively. Slit skin smears were collected from these patients which were transported to the laboratory for further processing. Ribonucleic acid was extracted by TRIzol method. Total Ribonucleic acid was used for real-time reverse transcription-polymerase chain reaction (two-step reactions). A standard sample with a known copy number was run along with unknown samples for a reverse transcription-polymerase chain reaction. Patients were further assessed for their clinical and molecular parameters during 6th month and 12th month of therapy. Results: All 78 new cases showed the presence of a viable load of bacilli at the time of recruitment, but we were able to follow up only on 36 of these patients for one year. Among these, using three different genes, 20/36 for esxA, 22/36 for hsp18 and 24/36 for 16S rRNA cases showed viability of M. leprae at the time of completion of 12 months of multidrug therapy treatment. All these positive patients were histopathologically active and had bacillary indexes ranging between 3+ and 4+. Patients with a high copy number of the Mycobacterium leprae gene, even after completion of treatment as per WHO recommended fixed-dose multidrug therapy, indicated the presence of live bacilli. Limitations: Follow up for one year was difficult, especially in Delhi because of the migratory nature of the population. Patients who defaulted for scheduled sampling were not included in the study. Conclusion: The presence of a viable load of bacilli even after completion of therapy may be one of the reasons for relapse and continued transmission of leprosy in the community.

Human Skin as an Ex Vivo Model for Mycobacterium leprae Maintenance and Studies of Leprosy

“…More recently, Collins and collaborator [38] mention that 16S rRNA is a stable marker, that could still be detected in dead bacilli. However, 16S rRNA has been used by other researchers to evaluate the viability of M. leprae in clinical, environmental and laboratory samples [14,[39][40][41][42][43][44]. Here, we used 16S rRNA RT-PCR to showing molecular viability based on the presence of rRNA until the 60th day of the hOSEC incubation period; furthermore, to corroborate this find, we demonstrated that M. leprae, cultivated in the hOSEC, beyond maintaining viability, also had its infectivity preserved because it was able to infect the footpad of several athymic mice.…”

Human Skin as an Ex Vivo Model for Maintaining Mycobacterium leprae and Leprosy Studies

“…More recently, Collins and collaborators [ 38 ] mention that 16S rRNA is a stable marker that could still be detected in dead bacilli. However, 16S rRNA has been used by other researchers to evaluate the viability of M. leprae in clinical, environmental, and laboratory samples [ 14 , 39 , 40 , 41 , 42 , 43 , 44 ]. Here, we used 16S rRNA RT-PCR to show molecular viability based on the presence of rRNA until the 60th day of the hOSEC incubation period; furthermore, to corroborate this finding, we demonstrated that M. leprae , cultivated in hOSEC, beyond maintaining viability, also had its infectivity preserved because it was able to infect the foot pad of several athymic mice.…”