Efficacy of flavourzyme against Salmonella Typhimurium, Escherichia coli, and Pseudomonas aeruginosa biofilms on food-contact surfaces

Begum, Shameem Ara; Ha, Angela Jie-won; Byun, Kye-Hwan; Hossain, Iqbal; Mizan, Md. Furkanur Rahaman; Ha, Sang‐Do

doi:10.1016/j.ijfoodmicro.2020.108897

Cited by 30 publications

(14 citation statements)

References 42 publications

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“…Foodborne bacteria persistence in food processing environments is the principal source of food contamination, resulting in substantial issues and significant financial losses for the food industry. Due to concerns regarding the innocuity of some synthetic food preservatives, natural substances are quickly replacing chemical-based sanitizers and disinfectants [ 11 ]. Quercetin is present in plant extracts that could be considered as food ingredients rather than food additives.…”

Section: Discussionmentioning

confidence: 99%

“…However, when quercetin inhibited the biofilm, the inhibitory effect was greater on R surfaces than on HG surfaces at MIC of quercetin ( Figure 3 and Figure 4 ). The use of plastic cutting boards for processing and cooking raw foods is very susceptible to cross-contamination [ 50 ] because Salmonella can adhere to the surfaces of plastic to form a biofilm [ 11 ]. Additionally, because plastic is a hydrophobic material, Salmonella bacteria are more likely to adhere on it than on glass and stainless steel surfaces, which are hydrophilic materials [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…As a result, biofilm, which is difficult to remove, is a significant hygiene issue in the food industry [ 9 ]. Food spoilage and pathogenic bacteria have ability to adhere to food-processing surfaces, such as stainless steel (SS), silicon rubber (SR), plastic (PLA), rubber gloves (RG), and food surfaces, and biofilm formation is a serious public health concern since resistant biofilms can be a persistent source of contamination [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Roy

Song

Park

2022

Foods

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Quercetin is an active nutraceutical element that is found in a variety of foods, vegetables, fruits, and other products. Due to its antioxidant properties, quercetin is a flexible functional food that has broad protective effects against a wide range of infectious and degenerative disorders. As a result, research is required on food-contact surfaces (rubber (R) and hand gloves (HG)) that can lead to cross-contamination. In this investigation, the inhibitory effects of quercetin, an antioxidant and antibacterial molecule, were investigated at sub-MIC (125; 1/2, 62.5; 1/4, and 31.25; 1/8 MIC, μg/mL) against Salmonella Typhimurium on surfaces. When quercetin (0–125 μg/mL) was observed on R and HG surfaces, the inhibitory effects were 0.09–2.49 and 0.20–2.43 log CFU/cm2, respectively (p < 0.05). The results were confirmed by field emission scanning electron microscopy (FE-SEM), because quercetin inhibited the biofilms by disturbing cell-to-cell connections and inducing cell lysis, resulting in the loss of normal cell morphology, and the motility (swimming and swarming) was significantly different at 1/4 and 1/2 MIC compared to the control. Quercetin significantly (p < 0.05) suppressed the expression levels of virulence and stress response (rpoS, avrA, and hilA) and quorum-sensing (luxS) genes. Our findings imply that plant-derived quercetin could be used as an antibiofilm agent in the food industry to prevent S. Typhimurium biofilm formation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Roy

Song

Park

2022

Foods

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“…After treatment, data were recorded by 64-bit high-performance FT-IR software. Documented data were aligned and the wavenumbers of the major spectra were detected by OMNIC™ Spectra Software (Thermo Fisher Scientific) in transmission (%T) mode [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

Development and efficacy assessment of hand sanitizers and polylactic acid films incorporating caffeic acid and vanillin for enhanced antiviral properties against HCoV-229E

Hyun,

Han,

Son

et al. 2023

Virol J

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Background Although three years after the outbreak of SARS-CoV-2, the virus is still having a significant impact on human health and the global economy. Infection through respiratory droplets is the main transmission route, but the transmission of the virus by surface contact cannot be ignored. Hand sanitizers and antiviral films can be applied to control SARS-CoV-2, but sanitizers and films show drawbacks such as resistance of the virus against ethanol and environmental problems including the overuse of plastics. Therefore, this study suggested applying natural substrates to hand sanitizers and antiviral films made of biodegradable plastic (PLA). This approach is expected to provide advantages for the easy control of SARS-CoV-2 through the application of natural substances. Methods Antiviral disinfectants and films were manufactured by adding caffeic acid and vanillin to ethanol, isopropyl alcohol, benzalkonium chloride, and PLA. Antiviral efficacies were evaluated with slightly modified international standard testing methods EN 14,476 and ISO 21,702. Results In suspension, all the hand sanitizers evaluated in this study showed a reduction of more than 4 log within 2 min against HCoV-229E. After natural substances were added to the hand sanitizers, the time needed to reach the detection limit of the viral titer was shortened both in suspension and porcine skin. However, no difference in the time needed to reach the detection limit of the viral titer was observed in benzalkonium chloride. In the case of antiviral films, those made using both PLA and natural substances showed a 1 log reduction of HCoV-229E compared to the neat PLA film for all treatment groups. Furthermore, the influence of the organic load was evaluated according to the number of contacts of the antiviral products with porcine skin. Ten rubs on the skin resulted in slightly higher antiviral activity than 50 rubs. Conclusion This study revealed that caffeic acid and vanillin can be effectively used to control HCoV-229E for hand sanitizers and antiviral films. In addition, it is recommended to remove organic matter from the skin for maintaining the antiviral activity of hand sanitizer and antiviral film as the antiviral activity decreased as the organic load increased in this study.

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“…As spoilage bacteria, the Gram-negative Pseudomonas aeruginosa (P. aeruginosa) is a major contributor to the spoilage of aquatic products, meats, and cold ready-to-eat foods [1][2][3]. Simultaneously, P. aeruginosa is also a conditional pathogen and a common agent causing chronic infection in immunocompromised patients [4,5].…”

Section: Introductionmentioning

confidence: 99%

Application of the Luminescent luxCDABE Gene for the Rapid Screening of Antibacterial Substances Targeting Pseudomonas aeruginosa

Peng

Wang

Zhu

et al. 2023

Foods

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Pseudomonas aeruginosa (P. aeruginosa) is a typical Gram-negative bacterium that can cause the spoilage of catered food products. Using a luminescent reporter gene (luxCDABE), this study sought to construct a cell-based biosensor (PAO1-CE) to rapidly screen antibacterial substances against P. aeruginosa. A total of six antibiotics belonging to five categories were used as the model test substances. The results of the bioluminescence detection method were verified using traditional antibacterial research assessments. The correlation coefficient of the regression equation fitting the data generated using this method was greater than 0.98, supporting the credibility of this approach. Additionally, the EC50 of each of the antibiotics assessed in this study was lower than the 1/2 MIC determined by conventional means. All six of the antibiotics caused varying degrees of damage to the cell membrane and cell wall of P. aeruginosa. Importantly, this novel method helped shorten the time necessary for active-compound detection and could be used for high-throughput detection, which would also help improve the detection efficiency. The application of this method towards the discovery of novel antibacterial compounds targeting P. aeruginosa holds substantial promise for greatly improving the efficiency of compound discovery.

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Efficacy of flavourzyme against Salmonella Typhimurium, Escherichia coli, and Pseudomonas aeruginosa biofilms on food-contact surfaces

Cited by 30 publications

References 42 publications

Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Impact of Quercetin against Salmonella Typhimurium Biofilm Formation on Food–Contact Surfaces and Molecular Mechanism Pattern

Development and efficacy assessment of hand sanitizers and polylactic acid films incorporating caffeic acid and vanillin for enhanced antiviral properties against HCoV-229E

Application of the Luminescent luxCDABE Gene for the Rapid Screening of Antibacterial Substances Targeting Pseudomonas aeruginosa

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