Efficacy of fluorochrome stain in the diagnosis of pulmonary tuberculosis co-infected with HIV

Abstract

Fluorochrome staining was found to be more efficient (45%) when compared to ZN staining (29%) in detecting cases associated with HIV seropositivity, especially paucibacillary cases.

Determination of fluorescent stain and Ziehl - Nelson stain for the demonstration of acid fast bacilli in cytological specimens

“…Our results were similar with. Prasanti K, 9 Cheng AG, 10 Sethi S, 11 Singh NP, 12 Ulukanligil M, 13 and Jain A 14 in their studies which also found AR stain was more sensitive in comparison with ZN stain for the diagnosis of tubercular bacilli. Thus it is concluded that, for detection of AFB in paucibacillary specimen, AR is much more sensitive than ZN staining technique.…”

Evaluation of various culture and staining techniques for the detection of extra pulmonary tuberculosis

“…staining, 32% by ZN staining). [6][7][8][9][10] The use of fluorescent stain greatly improves the diagnostic value of the EPTB smears with a low density of bacilli (Paucibacillary) which are likely to be missed on ZN stained smear. This study showed the higher isolation rate of MTB complex in MGIT culture system (96.29%) than the conventional LJ medium culture assay (81.48%).…”

Novel Developments in the Epidemic of Human Immunodeficiency Virus and Tuberculosis Coinfection

“…Although smear microscopy is less frequently positive in patients infected with HIV and TB, advances in fluorescence microscopy (94)(95)(96)(97) and optimization of smear preparation (98)(99)(100)(101), coupled with important steps toward so-called frontloaded microscopy, in which two samples are taken on the same day (102), ensure that smear microscopy remains the mainstay of diagnosis of TB globally. However, new diagnostic tools for the detection of TB and drug-resistant TB have been developed on the basis of both phenotypic and genotypic methods.…”