Efficacy of genetically transformed Metarhizium anisopliae against Spodoptera litura and Aphis craccivora

Kumar, Anoop; Suroshe, Sachin S.; Sonam, -; Saini, Gurvinder Kaur; Singh, Rajesh

doi:10.1016/j.sjbs.2022.103493

Cited by 2 publications

(3 citation statements)

References 34 publications

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“…According to our findings, the LT 50 of B. bassiana was reduced by 5.58 and 1.76 times, after leaf treatment and spray technique, respectively in A. craccivora . Similarly, the median lethal time of genetically modified M. anisopliae (MaLqqIT1-7) was reduced by 2.83 and 3.06 times against the S. litura and A. craccivora , respectively at 10 7 conidia/ml fungal concentration ( Kumar et al, 2023 ). However, the LT 50 of L. lecanii expressing BmKit from Buthus martensii (Karsch) reduced by 26.5% against Aphis gossypii (Glover) ( Xie et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…A. craccivora were collected from the infested cowpea ( Vigna unguiculata (Linnaeus) Walpers) plants from the fields of ICAR-IARI, Pusa, New Delhi. Aphids were reared on to the 3–5 days old, fresh and tender cowpea seedlings grown in the plastic jars (15.5 cm θ) using absorbant cotton ( Kumar et al, 2023 ). This population was maintained as a stock culture throughout the study period.…”

Section: Methodsmentioning

confidence: 99%

“…Genetically transformed B. bassiana was subcultured as per the previously described method ( Kumar et al, 2023 ). Procured fungal source (aqueous fungal suspension) was subcultured on standard Sabouraud Dextrose Agar medium (SDA) (Himedia) in full darkness at 27 ± 1 °C and 80 ± 5% RH.…”

Section: Methodsmentioning

confidence: 99%

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Bioefficacy of engineered Beauveria bassiana with scorpion neurotoxin, LqqIT1 against cotton mealybug, Phenacoccus solenopsis and cowpea aphid, Aphis craccivora

Rajput,

Suroshe,

Yadav

et al. 2023

PeerJ

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Cotton mealybug, Phenacoccus solenopsis (Tinsley) and cowpea aphid Aphis craccivora (Koch) are notorious polyphagous, hemipteran sap sucking insect pests. A recombinant toxin gene ‘LqqIT1’ from the scorpion Leiurus quinquestriatus quinquestriatus (Ehrenberg) was cloned in the pAL1 fungal expression vector and then expressed in the entomopathogenic fungus Beauveria bassiana (Balasmo) using genetic modification techniques. The genetically transformed B. bassiana strain (BbLqqIT1-3) and its un-transformed parent strain (Bb-C) were screened to infect the third instar nymphs of P. solenopsis and first instar nymph of A. craccivora through leaf treatment and topical application (spray) method at 1 * 107 spores per ml concentration. The recombinant strain BbLqqIT1-3 was highly pathogenic against A. craccivora but non pathogenic to P. solenopsis. BbLqqIT1-3 induced 72 and 43.33% mortality in A. craccivora nymphs 96 h after leaf treatment and topical application, respectively. The nymphs of A. craccivora infected with BbLqqIT1-3 displayed classical neurotoxic symptoms such as sluggishness, solublize and liquification of the body. Crude soluble toxin protein, BbLqqIT1a-CSE and Bb-WT-CSE was extracted from the BbLqqIT1-3 and Bb-C, respectively using ammonium sulphate precipitation method, and their oral toxicity was analyzed at 5 µg/ml concentration. The survival of the studied insects was negatively affected by the crude soluble toxin extracts. The LT50 values of BbLqqIT1a-CSE against P. solenopsis and A. craccivora were 22.18 and 17.69 h, respectively. Exposure to crude soluble toxin extracts also accounted for the imbalance of ionic concentrations in the hemolymph of treated insects such as hyperpotassemia (3.53–8.18 meq/ml) in the P. solenopsis and hypopotassemia (7.52–0.47 meq/ml) in A. craccivora. The transformed fungus BbLqqIT1-3 strain exhibited promising results in invitro study.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Bioefficacy of engineered Beauveria bassiana with scorpion neurotoxin, LqqIT1 against cotton mealybug, Phenacoccus solenopsis and cowpea aphid, Aphis craccivora

Rajput,

Suroshe,

Yadav

et al. 2023

PeerJ

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Managing Super Pests: Interplay between Pathogens and Symbionts Informs Biocontrol of Whiteflies

Yan,

Wang,

Liu

et al. 2024

Microorganisms

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Bemisia tabaci is distributed globally and incurs considerable economic and ecological costs as an agricultural pest and viral vector. The entomopathogenic fungus Metarhizium anisopliae has been known for its insecticidal activity, but its impacts on whiteflies are understudied. We investigated how infection with the semi-persistently transmitted Cucurbit chlorotic yellows virus (CCYV) affects whitefly susceptibility to M. anisopliae exposure. We discovered that viruliferous whiteflies exhibited increased mortality when fungus infection was present compared to non-viruliferous insects. High throughput 16S rRNA sequencing also revealed significant alterations of the whitefly bacterial microbiome diversity and structure due to both CCYV and fungal presence. Specifically, the obligate symbiont Portiera decreased in relative abundance in viruliferous whiteflies exposed to M. anisopliae. Facultative Hamiltonella and Rickettsia symbionts exhibited variability across groups but dominated in fungus-treated non-viruliferous whiteflies. Our results illuminate triangular interplay between pest insects, their pathogens, and symbionts—dynamics which can inform integrated management strategies leveraging biopesticides This work underscores the promise of M. anisopliae for sustainable whitefly control while laying the groundwork for elucidating mechanisms behind microbe-mediated shifts in vector competence.

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Efficacy of genetically transformed Metarhizium anisopliae against Spodoptera litura and Aphis craccivora

Cited by 2 publications

References 34 publications

Bioefficacy of engineered Beauveria bassiana with scorpion neurotoxin, LqqIT1 against cotton mealybug, Phenacoccus solenopsis and cowpea aphid, Aphis craccivora

Bioefficacy of engineered Beauveria bassiana with scorpion neurotoxin, LqqIT1 against cotton mealybug, Phenacoccus solenopsis and cowpea aphid, Aphis craccivora

Managing Super Pests: Interplay between Pathogens and Symbionts Informs Biocontrol of Whiteflies

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