Efficacy of heterologous and homologous heat shock protein 70s as protective agents to Artemia franciscana challenged with Vibrio campbellii

Baruah, Kartik; Ranjan, Jayant; Sorgeloos, Patrick; Bossier, Peter

doi:10.1016/j.fsi.2010.07.011

Cited by 47 publications

(46 citation statements)

References 45 publications

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“…The data presented here begin to elucidate the innate immune system of P. martensii by focusing on the role of a conserved protein known to be involved in various stress responses. A better understanding these response mechanisms might alleviate some of the strain on developing aquaculture because diseases caused by bacteria or viruses are still considered as a major constraint to the sustainable development of aquaculture worldwide (Baruah et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Lei¹,

Wu²,

Liang³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Small heat shock proteins (HSPs) are molecular chaperones with ATP-independent properties. They are involved in a variety of physiological and stress processes. In this study, the full-length HSP 20 (HSP20) from Pinctada martensii, designated as PmHSP20, was obtained from hemocytes using rapid amplification of cDNA ends technology. The PmHSP20 cDNA was 952 bp in length, containing an open reading frame of 534 bp that encoded 177-amino acid residues, with an isoelectric point of 5.86 and molecular weight of 20.24 kDa. The sequence of this deduced polypeptide contained typical structure and function domains conserved in the HSP20 family, providing evidence that PmHSP20 belongs to the HSP20 family. The PmHSP20 mRNA expression levels were detected in various tissues of P. martensii and in hemocytes after challenges with the bacteria Vibrio harveyi and lipopolysaccharide (LPS) using quantitative realtime polymerase chain reaction amplification. The results indicated that PmHSP20 is constitutively expressed in all tissues tested and might be involved in the immune response. The upregulation of PmHSP20 after V. harveyi and LPS challenge suggests that PmHSP20 plays an important role in anti-bacterial immunity. Studies on PmHSP20 are a valuable resource to further explore the immune system in pearl oysters and might enhance our knowledge of molluscan innate immunity.

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Section: Discussionmentioning

confidence: 99%

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Lei¹,

Wu²,

Liang³

et al. 2016

Genet. Mol. Res.

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“…Diseases caused by pathogenic or opportunistic bacteria such as Vibrio spp are still considered a major hurdle in the sustainable development of aquaculture worldwide (Baruah et al, 2010). Expression profiles of Tg-GS in hemocytes after V. parahaemolyticus and LPS challenge are shown in Figure 5.…”

Section: Discussionmentioning

confidence: 99%

Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides

Bao¹,

Li²,

Ye³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The blood cockle, Tegillarca granosa, is a widely consumed clam in the Indo-Pacific region. Glutamine synthetase (GS) is an enzyme that plays an essential role in the metabolism of nitrogen by catalyzing the condensation of glutamate and ammonia to form glutamine. We identified the GS of T. granosa (Tg-GS) from hemocytes by 3'-and 5'-rapid amplification of cDNA ends (RACE)-PCR. The fulllength cDNA consisted of 1762 bp, with a 1104-bp open reading frame encoding 367 amino acids. Sequence comparison showed that Tg-GS has homology to GS of other organisms, with 79.78% identity with GS from the Pacific oyster Crassostrea gigas, 71.98% identity with GS from the zebrafish Danio rerio, and 68.96% identity with human Homo sapiens GS. A C-beta-Grasp domain and an N-catalytic domain were identified in Tg-GS, indicating that Tg-GS should be classified as a new member of the GS family. A quantitative RT-PCR assay was used to detect mRNA expression of Tg-GS in five different tissues. Higher levels of mRNA expression of GS were detected in the tissues of hemocytes and the mantle. Up-regulation of GS by challenge with the bacteria Vibrio parahaemolyticus and with bacterial wall lipopolysaccharides showed that GS plays a role in anti-bacterial immunity. We conclude that pathogen infection significantly induces expression level of Tg-GS, and that activation of GS influences the immune response of T. granosa by increasing glutamine concentration.

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“…Following recovery period, groups of 20 larvae from the 250-ml glass bottles were transferred to sterile 40-ml glass tubes that contained 10 ml of sterile artificial seawater (35 g/l salinity). Subsequently, it was verified whether such pretreatment can protect the larvae against subsequent challenge with pathogenic V. harveyi at 10 7 cells/ml (Baruah et al 2010) or against a lethal heat stress at 41°C for 20 min (Baruah et al 2012). The survival of Artemia larvae was scored manually at an indicated period after challenge as described previously (Baruah et al 2015b).…”

Section: In Vivo Pre-pretreatment Of Artemiamentioning

confidence: 99%

Enhanced resistance against Vibrio harveyi infection by carvacrol and its association with the induction of heat shock protein 72 in gnotobiotic Artemia franciscana

Baruah

Norouzitallab

Phong

et al. 2017

Cell Stress and Chaperones

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Induction of HSP72 is a natural response of stressed organisms that protects against many insults including bacterial diseases in farm (aquatic) animals. It would therefore be of great health benefit to search for natural compounds that are clinically safe yet able to induce HSP72 in animals. The phenolic compound carvacrol, an approved food component, had been shown in in vitro study to act as a coinducer of HSP72, enhancing HSP72 production only in combination with a bona fide stress compared to the compound alone. However, in vitro model systems do not completely represent an in vivo physiology. Here, using the wellestablished gnotobiotic Artemia model system, we determined whether carvacrol could induce HSP72 in vivo, whether this putative effect could generate resistance in Artemia against biotic/abiotic stress and also unraveled the mechanism behind the possible HSP72-inducing effect of carvacrol. The gnotobiotic system is crucial for such studies because it avoids the interference of any extraneous factors on host-compound interaction. Here, carvacrol was shown to be a potent HSP72 inducer. Induction of HSP72 was associated with the generation of resistance in Artemia larvae against subsequent lethal heat stress or pathogenic Vibrio harveyi. Our results also provided new insight on the mode of HSP72 inducing action of carvacrol, in which the initial generation of reactive molecule H 2 O 2 by the compound plays a key role. Overall results add new information about the bioactivity of carvacrol and advance our knowledge of this compound as potential prophylactic agent for controlling Vibrio infection in aquaculture animals.

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Efficacy of heterologous and homologous heat shock protein 70s as protective agents to Artemia franciscana challenged with Vibrio campbellii

Cited by 47 publications

References 45 publications

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Molecular cloning and expression analysis of heat shock protein 20 (HSP20) from the pearl oyster Pinctada martensii

Expression of glutamine synthetase in Tegillarca granosa (Bivalvia, Arcidae) hemocytes stimulated by Vibrio parahaemolyticus and lipopolysaccharides

Enhanced resistance against Vibrio harveyi infection by carvacrol and its association with the induction of heat shock protein 72 in gnotobiotic Artemia franciscana

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