Efficacy of Human Adipose Tissue-Derived Stem Cells on Neonatal Bilirubin Encephalopathy in Rats

Abstract

Kernicterus is a neurological syndrome associated with indirect bilirubin accumulation and damages to the basal ganglia, cerebellum and brain stem nuclei particularly the cochlear nucleus. To mimic haemolysis in a rat model such that it was similar to what is observed in a preterm human, we injected phenylhydrazine in 7-day-old rats to induce haemolysis and then infused sulfisoxazole into the same rats at day 9 to block bilirubin binding sites in the albumin. We have investigated the effectiveness of human adiposity-derived stem cells as a therapeutic paradigm for perinatal neuronal repair in a kernicterus animal model. The level of total bilirubin, indirect bilirubin, brain bilirubin and brain iron was significantly increased in the modelling group. There was a significant decreased in all severity levels of the auditory brainstem response test in the two modelling group. Akinesia, bradykinesia and slip were significantly declined in the experience group. Apoptosis in basal ganglia and cerebellum were significantly decreased in the stem cell-treated group in comparison to the vehicle group. All severity levels of the auditory brainstem response tests were significantly decreased in 2-month-old rats. Transplantation results in the substantial alleviation of walking impairment, apoptosis and auditory dysfunction. This study provides important information for the development of therapeutic strategies using human adiposity-derived stem cells in prenatal brain damage to reduce potential sensori motor deficit.

Combination of laser and human adipose-derived stem cells in repair of rabbit anal sphincter injury: a new therapeutic approach

“…Some animal studies show that xenograft transplantation of mesenchymal stem cells can reduce the immunomodulation [48] and efficacy of these cells [49]. However, the use of hADSCs has been usual in preclinical studies [50–52] and its efficacy is proven. The findings of the present study also show that xenograft transplantation of hADSCs in the anal sphincterotomy model in rabbit improves sphincter function and tissue repair.…”

Co-administration of human adipose-derived stem cells and low-level laser to alleviate neuropathic pain after experimental spinal cord injury

“…Cell therapy is another non-drug measure that is currently the center of attention for treating neural diseases [21][22][23] including neuropathic pain [24,25]. Clinical use of mesenchymal stem cells (MSCs) with the aim of treating various diseases [22,[26][27][28] is increasing day by day since its safety and efficacy have been proven [29,30].…”

Differentiation of Mesenchymal Stem Cells Derived From Human Adipose Tissue Into Cholinergic-like Cells; in vitro

“…In our research, we demonstrated that isolated hAD-MSCs expressed typical levels of MSCs markers like CD105, CD29, CD90, CD73, and CD44. On the other hand, the absence of expression of CD45, CD31, CD34, and HLADR markers distinguished AD-MSCs from hematopoietic and endothelial cell types ( Amini et al, 2016 ).…”