Efficacy of human chorionic gonadotropin (hCG) and crude pituitary extract of fish and frog in oocyte maturation and ovulation in African catfish, Clarias gariepinus Burchell, 1822 and Clarias anguillaris L., 1762

Abstract

Abstract. The African catfish, Clarias gariepinus Burchell and Clarias anguillaris L., were induced to ovulate in aquaria by the administration of human chorionic gonadotropin (hCG) and pituitary extracts of Clarias albopunctatus (Nichols & Lamonte) and frog, Rana elegans. For final maturation and ovulation in both species of Clarias, the total dosage of each hormone was inversely proportional to the initial oocyte diameters of the recipient females. The time of ovulation after the last injection (latent period) was significantly shorter (11–13h) in females with large oocyte diameters (>920μm) than in those whose mean oocyte diameters were less than 920 μm (13–16h) (P < 0·05). The mean dosage of each test hormone required to induce ovulation was lower in females with large oocyte diameters than in those with smaller oocyte diameters (<920μm). The mean percentage hatch did not depend significantly on the nature of the treatment (P > 0μ05) and was about 86%. Clarias albopunclatus and R. elegans, which are economically unimportant and abundant in Anambra River basin, Nigeria, could be used as effective sources of hypophyseal factors in Clarias breeding programmes.

Artificial propagation of African catfish (Clarias gariepinus): the application of a single dose of pellets containing D-Ala6,Pro9NEt-mGnRH and dopamine inhibitor metoclopramide

“…It is also worth noting that in spite of numerous studies on the ovulation stimulation in this species with various preparations (Hogendoorn, 1979;De Leeuw et al, 1985;Richter et al, 1985;Richter et al, 1987a,b;Goos et al, 1987;Kouřil et al, 1992;Inyang and He�iarachchi, 1994;Brzuska, 2002) none of them is used in practice on a larger scale. Hence it seems that further studies on ovulation stimulation with Ovopel can be recommended.…”

Estudo comparativo da indução hormonal da espermiação em piavuçu (Leporinus macrocephalus) com extrato de hipófise de frango, coelho e carpa

“…O volume de sêmen, porém, varia, entre outros fatores, quanto ao indutor e à espécie que está sendo induzida, como é verificado no trabalho de Zaniboni Filho (1995), que induziu pacu (Piaractus mesopotamicus) com EHC, obtendo 12,1mL de sêmen e 14,4mL quando utilizou LHRH-a (fator liberador de hormônio luteínico-análogo). Kucharczyk et al (1997) obtiveram 4,3mL de sêmen de ide (Leuciscous idus L.) induzidos com EHC.…”

Induced Breeding of Two Clariid Catfishes Clurius gariepinus and Heterobranchus bidorsalis Using Tilapia Pituitary Extracts

“…Although pituitary extracts from non-piscine sources have also induced spawning in clariid catfishes C. anguillaris, C. gariepinus, C. isheriensis, H. longi$lis (Fagbenro et al 1992;Nwadukwe 1993;Inyang and Hettiarachchi 1994;Salami et al 1994) in Nigeria, the standardization of methods, dosages and concentrations of hormones are often inadequate. It is generally more efficient to induce ovulation in fishes with a pituitary gland extract or a gonadotropin from a teleostean source because of the phylogenetic closeness between the donor and recipient (Lam 1982).…”