Efficacy of<i>Bacillus subtilis</i>G-1 in suppression of stem rot caused by<i>Sclerotium rolfsii</i>and growth promotion of groundnut

Shifa, Hassen; Gopalakrishnan, C.; Velazhahan, R.

doi:10.5958/2230-732x.2015.00015.7

Cited by 13 publications

(8 citation statements)

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“…A number of commercial products based on B. subtilis including Kodiak (Gufstafson Biologicals, Plano, TX), Serenade (Agraquest Inc., Davis, CA), Subtilex (Becker Underwood, Ames, IA) have been developed for the control of various plant diseases (Schisler et al, 2004). Shifa et al (2015a) recently reported that seed treatment and soil application with the powder formulation of B. subtilis strain G1 effectively reduced the incidence of stem rot of groundnut caused by Sclerotium rolfsii and increased the pod yield. This strain was compatible with other beneficial rhizobacteria including Bacillus megaterium var phosphaticum strain PBS, Rhizobium strain BMBS, Azospirillum brasiliense strain 204 and Azotobacter chrooccocum strain AC1.…”

Section: Resultsmentioning

confidence: 99%

“…effectively controlled downy mildew of maize. In this study, the efficacy of various bacterial antagonists viz., Bacillus subtilis G-1, Bacillus amyloliquefaciens B2, Brevibacillus brevis (Bbv) 57 and Pseudomonas fluorescens Pf1 selected based on previously determined antagonistic activity against other soilborne fungal pathogens (Meena et al, 2001;Shifa et al, 2015a) was assessed for suppression of downy mildew of maize caused by P. sorghi under environmentally controlled conditions. The aim of the present study was to find out the efficacy of biocontrol agents viz., Bacillus subtilis G1, Bacillus amyloliquefaciens B2, Brevibacillus brevis 57 and Pseudomonas fluorescens Pf1 for the management of downy mildew of maize and for promoting plant growth was evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Biological control of downy mildew of maize caused by Peronosclerospora sorghi under environmentally controlled conditions

Yeturi

Velazhahan

2016

JANS

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Downy mildew disease, caused by Peronosclerospora sorghi, is one of the most serious diseases of maize. The disease is currently managed by seed treatment with metalaxyl fungicides. However, problems regarding environmental pollution resulting from the use of fungicides and development of fungicide resistance within populations of P. sorghi are of increasing concern. Assuming that biological control by means of using antagonistic microorganisms may be an alternative for the management of this disease, the efficacy of biocontrol agents viz., Bacillus subtilis G1, Bacillus amyloliquefaciens B2, Brevibacillus brevis 57 and Pseudomonas fluorescens Pf1 for the management of downy mildew of maize and for promoting plant growth was evaluated. The results indicated that seed treatment with B. subtilis G1 and B. amyloliquefaciens B2 significantly (P = 0.05) increased the germination percentage and seedling vigour of maize as assessed by roll towel method. Among them, B. subtilis G1 was the most effective and recorded 9% and 31% increases in germination percentage and seedling vigour of maize respectively, as compared to the control. A talc- based powder formulation of B. subtilis G1 when applied through seed at the rate of 10 g/kg reduced the downy mildew incidence up to 54% under greenhouse conditions. Results of this study suggest that B. subtilis G1 is a promising bioagent for the management of downy mildew of maize and for promoting plant growth. This antagonist could be further exploited for commercial scale up for ecofriendly management of downy mildew of maize under localized climatic conditions.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biological control of downy mildew of maize caused by Peronosclerospora sorghi under environmentally controlled conditions

Yeturi

Velazhahan

2016

JANS

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“…In direct confrontation assays, Shifa et al (2015) selected from seven biocontrol agents with antifungal activity the bacteria with the best results in the in vitro assay to control Sclerotium rolfsii. The biocontrol agent they selected was the bacteria B. subtilis.…”

Section: Greenhouse Testsmentioning

confidence: 99%

Biocontrol of Fusarium oxysporum f. sp. vasinfectum With Bacillus spp. Strains

Montalvão¹,

Castro²,

Blum³

et al. 2021

JAS

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The wilt caused by Fusarium oxysporum f.sp. vasinfectum in cotton is one of the main diseases of this crop. The pathogen is difficult to control because it produces chlamydospores, which allows it to survive in the soil for long periods without the presence of its main host. The control of this disease is based on varieties with some resistance, certified seeds, and fungicides used to treat seeds. Thus, the development of control alternatives is needed to increase the number of resources to manage this disease in the field. Bacillus are bioregulators and antagonists of several important phytopathogens. Therefore, the objective of this work was to select strains of Bacillus (Paenibacillus alvei, Bacillus thuringiensis, B. megaterium, and B. lentus), whose metabolites are toxic to F. oxysporum in laboratory and greenhouse conditions, in addition to adjusting in vitro methodologies to perform and evaluate selection tests. This work determined the best culture medium for the direct confrontation assays, proposed a grading scale to facilitate decisions about selection with the dual tests, identified among the strains selected in the confrontation assays those that produce volatile and non-volatile metabolites toxic to F. oxysporum, and tested these selected strains for disease control during seed germination in greenhouse trials. In last test, some strains of Bacillus had a potential protective effect during the germination of cotton seeds.

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“…The plates were incubated at room temperature (25 ± 2°C) for 3-5 days or until the mycelial growth in the control completely covered the Petri plate. After incubation, the radial growth of the pathogens was recorded and percent growth inhibition was calculated (Shifa et al 2015).…”

Section: Antagonistic Potential Of Bacterial Endophytesmentioning

confidence: 99%

Cyanide degradation and antagonistic potential of endophytic Bacillus subtilis strain BEB1 from Bougainvillea spectabilis Willd

et al. 2020

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2020)Cyanide degradation and antagonistic potential of endophytic Bacillussubtilis strain BEB1 from Bougainvilleaspectabilis Willd, All Life, 13:1, 92-98, ABSTRACTBacterial endophytes were isolated from the leaves of Bougainvillea spectabilis Willd. and screened for their in vitro antagonistic activity against three important phytopathogenic fungi viz., Neocosmospora keratoplastica, Stemphylium vesicarium and Rhizoctonia solani. Among the 36 endophytic bacterial isolates screened, three isolates designated as BEB1, BEB5 and BEB6 inhibited the mycelial growth of all the three plant pathogenic fungi tested and recorded more than 10% growth inhibition. Scanning electron microscopic observations of hyphae of the pathogenic fungi at the periphery of the inhibition zone in the dual culture plate revealed morphological abnormalities such as shrinkage, pit formation and loss of turgidity. These isolates were identified as Bacillus subtilis (BEB1) and Bacillus cereus (BEB5 and BEB6) based on the MALDI Biotyper analysis. The Bacillus subtilis strain BEB1, which exhibited the highest level of antagonism, was tested for its sensitivity to cyanide and potential to degrade NaCN. The results revealed that this strain was capable of growing in a medium containing 5 mM of NaCN with a degradation efficiency of 53.8%. This endophyte is valuable for exploitation as a biocontrol agent to control diseases especially in the cyanogenic plants and bioremediation of cyanide from the industrial effluent. ARTICLE HISTORY

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Efficacy ofBacillus subtilisG-1 in suppression of stem rot caused bySclerotium rolfsiiand growth promotion of groundnut

Cited by 13 publications

References 0 publications

Biological control of downy mildew of maize caused by Peronosclerospora sorghi under environmentally controlled conditions

Biological control of downy mildew of maize caused by Peronosclerospora sorghi under environmentally controlled conditions

Biocontrol of Fusarium oxysporum f. sp. vasinfectum With Bacillus spp. Strains

Cyanide degradation and antagonistic potential of endophytic Bacillus subtilis strain BEB1 from Bougainvillea spectabilis Willd

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