Efficacy of Bacillus subtilis and Trichoderma harzianum combination on chickpea Fusarium wilt caused by F. oxysporum f. sp. ciceris

Zaim, Souad; Bekkar, Ahmed Amine; Belabid, L.

doi:10.1080/03235408.2018.1447896

Cited by 36 publications

(17 citation statements)

References 16 publications

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“…Application of a compatible combination of B. subtilis MF352017 and T. harzianum controlled chickpea wilt caused by Fusarium oxysporum f. sp. ciceris and enhanced plant growth as compared to individual application (Zaim et al, 2018). Treatment with a combination of compatible B. subtillis ATCC 11774, T. harzianum and Trichoderma koningii suppressed the development of potato stem canker as well as promoted growth and yield (Ali et al, 2018).…”

Section: Bacillus-based Multistrain Mixturesmentioning

confidence: 99%

Multistrain versus single-strain plant growth promoting microbial inoculants - The compatibility issue

Thomloudi

Tsalgatidou

Douka

et al. 2019

Hellenic Plant Protection Journal

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Summary Plant Growth Promoting Microorganisms or Plant Probiotics (PGPMs) constitute a promising solution for agricultural sustainability. The concept that inoculation of PGPM mixtures may perform better in enhancing agricultural production than single strain application dates back to the discovery of plant growth rhizobacteria (PGPR) and is gaining ground in our days. This shift is highlighted by the increasing number of research publications dealing with the positive impact of microbial mixtures in promoting plant growth, controlling plant pathogens, as well as providing abiotic stress tolerance. The continuous deposition of patents as well as commercially available formulations concerning bioprotective and/or biostimulant multistrain mixtures also underlines this shift. A major issue in engineering an effective and consistent synthetic multistrain mixture appears to be the compatibility of its components. The present review provides a thorough literature survey supporting the view that treatment of plants with compatible multistrain mixtures generally exerts a better effect in plant growth and health than single-strain inoculation. Our study focuses on multistrain mixtures based on Pseudomonas, Bacillus and beneficial fungal strains, while commercial products are also being referred.

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Section: Bacillus-based Multistrain Mixturesmentioning

confidence: 99%

Multistrain versus single-strain plant growth promoting microbial inoculants - The compatibility issue

Thomloudi

Tsalgatidou

Douka

et al. 2019

Hellenic Plant Protection Journal

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“…Trichoderma-Bacillus combinations are among the well-documented examples in this context. Although co-cultivation of Trichoderna and Bacillus strains on artificial growth media was frequently characterized by antagonisms (Gyu Kim et al, 2008), in many plant species, including Oryza sativa (Ali and Nadarajah, 2014), Triticum aestivum (Karuppiah et al, 2019), Cicer arietinum (Zaim et al, 2018), Solanum melongena and Capsicum annuum (Abeysinghe, 2009), synergistic beneficial effects were reported after co-inoculation. This included stimulation of germination and growth promotion, as well as biocontrol effects against fungal pathogens, such as Rhizoctonia, Fusarium and Pythium, known as important damping-off diseases in cold and wet soils with potential relevance also for the present study.…”

Section: Introductionmentioning

confidence: 99%

Synergisms of Microbial Consortia, N Forms, and Micronutrients Alleviate Oxidative Damage and Stimulate Hormonal Cold Stress Adaptations in Maize

et al. 2020

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Aims: Low soil temperature in spring is a major constraint for the cultivation of tropical crops in temperate climates. This study aims at the exploitation of synergistic interactions of micronutrients, consortia of plant growth-promoting microorganisms and N forms as cold-stress protectants. Methods: Maize seedlings were exposed for two weeks to low root zone temperatures at 8-14 • C under controlled conditions on a silty clay-loam soil (pH 6.9) collected from a maize field cultivation site. A pre-selection trial with fungal and bacterial PGPM strains revealed superior cold-protective performance for a microbial consortium of Trichoderma harzianum OMG16 and Bacillus spp. with Zn/Mn supplementation (CombiA +), particularly in combination with N-ammonium as a starting point for the characterization of the underlying physiological and molecular mechanisms. Results: In nitrate-treated plants, the cold stress treatment increased oxidative leaf damage by 133% and reduced the shoot biomass by 25%, related with reduced acquisition of phosphate (P), zinc (Zn) and manganese (Mn). The supplying of N as ammonium improved the Zn and Mn nutritional status and increased the ABA shoot concentration by 33%, as well as moderately increased detoxification of reactive oxygen species (ROS). Moreover, use of N as ammonium also increased the root auxin (IAA) concentration (+76%), with increased expression of auxin-responsive genes, involved in IAA synthesis (ZmTSA), transport (ZmPIN1a), and perception (ZmARF12). Additional inoculation with the microbial consortium promoted root colonization with the inoculant strain T. harzianum OMG16 in combination with ammonium fertilization (+140%). An increased ABA/cytokinin ratio and increased concentrations of jasmonic (JA) and salicylic acids (SA) were related to a further increase in enzymatic and non-enzymatic ROS detoxification. Additional supplementation with Zn and Mn further increased shoot

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“…Combining two or more agents in biocontrol is an efficient strategy for the management of soilborne pathogens and has been reported in previous studies ( Awasthi et al, 2011 ; Yobo et al, 2011 ; Zaim et al, 2018 ; Jangir et al, 2019 ). To our knowledge, this study is the first report on combined application of an antagonistic B .…”

Section: Discussionmentioning

confidence: 70%

“…Combined use of two or more biocontrol candidates, a combination of bacterial–bacterial, bacterial–fungal, or fungal–fungal isolates, in managing various important plant diseases has been used for many years ( Awasthi et al, 2011 ; Yobo et al, 2011 ; Zaim et al, 2018 ; Jangir et al, 2019 ; Hansen et al, 2020 ). These methods also showed better efficacy compared to using a single beneficial microbe ( Kohler et al, 2007 ; Awasthi et al, 2011 ; Jangir et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced Biocontrol of Cucumber Fusarium Wilt by Combined Application of New Antagonistic Bacteria Bacillus amyloliquefaciens B2 and Phenolic Acid-Degrading Fungus Pleurotus ostreatus P5

Wang

Cai

et al. 2021

Front. Microbiol.

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Continuous monoculture of cucumber (Cucumis sativus L.) typically leads to the frequent incidence of Fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (FOC). As potent allelochemicals, phenolic acids are believed to be associated with soilborne diseases. This study aimed to investigate the effect of single or co-inoculation of antagonistic bacteria Bacillus amyloliquefaciens B2 and phenolic acid-degrading fungus Pleurotus ostreatus P5 on the suppression of cucumber Fusarium wilt. The strain B2 was identified as B. amyloliquefaciens based on biochemical, physiological, and 16S rDNA and gyrB gene sequence analyses. Strain B2 showed indole-3-acetic acid (IAA) and siderophore production and phosphate solubilization in in vitro assays. Scanning electron microscope (SEM) imaging showed the ability of strain B2 to adhere to the root surface of cucumber. P. ostreatus P5 could effectively degrade mixed phenolic acids as its sole source of carbon and energy for growth in liquid medium. In a pot experiment, four treatments were established as follows: (1) CK, uninoculated control; (2) B2, inoculation of strain B2; (3) P5, inoculation of strain P5; and (4) B2 + P5, co-inoculation of strain B2 and strain P5. At the end of the 60-day pot experiment, the B2, P5, and B2 + P5 treatments significantly reduced disease incidence by 48.1, 22.2, and 63.0%, respectively, compared to the CK treatment (p < 0.05). All three inoculation treatments significantly increased the growth of cucumber seedlings and suppressed the FOC population compared to the control (p < 0.05). High-performance liquid chromatography (HPLC) analysis showed that total phenolic acids were decreased by 18.9, 35.9, and 63.2% in the B2, P5, and B2 + P5 treatments, respectively. The results from this study suggest that combined application of B. amyloliquefaciens B2 and P. ostreatus P5 could be a promising strategy for suppressing Fusarium wilt and improving plant growth of cucumber seedlings under continuous cropping conditions.

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Efficacy of Bacillus subtilis and Trichoderma harzianum combination on chickpea Fusarium wilt caused by F. oxysporum f. sp. ciceris

Cited by 36 publications

References 16 publications

Multistrain versus single-strain plant growth promoting microbial inoculants - The compatibility issue

Multistrain versus single-strain plant growth promoting microbial inoculants - The compatibility issue

Synergisms of Microbial Consortia, N Forms, and Micronutrients Alleviate Oxidative Damage and Stimulate Hormonal Cold Stress Adaptations in Maize

Enhanced Biocontrol of Cucumber Fusarium Wilt by Combined Application of New Antagonistic Bacteria Bacillus amyloliquefaciens B2 and Phenolic Acid-Degrading Fungus Pleurotus ostreatus P5

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