Efficacy of Inactivated Equine Influenza H3N8 Vaccine Adjuvanted with Montanide Pet Gel A

Abstract

This study demonstrated the use of MONTANIDE PET GEL A as polymeric adjuvant in locally prepared inactivated Equine influenza (EI) vaccine. Safety and potency of the prepared vaccine were studied by inoculation into guinea pigs and horses and evaluated using Haem agglutination inhibition (HI) test. The vaccine was proved safe and potent for horses and guinea pigs. The mean HI antibody titers in sera of guinea pigs and horses at 3 weeks post vaccination were (752.4 and 144), respectively. The antibody titers in vaccinated horses were monitored where they lasted in their sera within the protective level (80) up to 9 months post vaccination. Therefore, we recommended the use of Montanide pet gel A as adjuvant for horse vaccines.