Efficacy of inactivated swine influenza virus vaccines against the 2009 A/H1N1 influenza virus in pigs

Vincent, Amy L.; Zanella, J. R. C.; Lorusso, Alessio; Gauger, Philip C.; Zanella, Eraldo Lourenso; Kehrli, Marcus E.; Janke, Bruce H.; Lager, Kelly M.

doi:10.1016/j.vaccine.2010.01.049

Cited by 86 publications

(80 citation statements)

References 21 publications

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“…Clinical observations and rectal temperatures were recorded individually on day Ϫ2 through day zero in order to establish normal baseline values for each animal. Nasal swabs were collected before vaccination and daily for 3 days as previously described (69,70). Following inoculation, rectal temperatures and clinical signs of disease were monitored daily until the terminus of the experiment (3 dpi), when the animals were humanely euthanized and necropsy was performed.…”

Section: Ethicsmentioning

confidence: 99%

Modifications in the Polymerase Genes of a Swine-Like Triple-Reassortant Influenza Virus To Generate Live Attenuated Vaccines against 2009 Pandemic H1N1 Viruses

Pena

Vincent

et al. 2011

J Virol

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128

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Section: Ethicsmentioning

confidence: 99%

Modifications in the Polymerase Genes of a Swine-Like Triple-Reassortant Influenza Virus To Generate Live Attenuated Vaccines against 2009 Pandemic H1N1 Viruses

Pena

Vincent

et al. 2011

J Virol

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128

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“…Nasal swabs were collected from five sows and four piglets showing respiratory signs. Nasal swabbing was performed using a rayon swab with a plastic handle that was placed in a viral transport medium (Vincent et al 2010). The swabs were refrigerated and submitted to the virology laboratory of Embrapa Swine and Poultry (CNPSA, Concordia, Santa Catarina, Brazil).…”

Section: Methodsmentioning

confidence: 99%

“…Several experimental studies demonstrated the susceptibility of pigs to this novel virus, ensuing a fast and efficient viral spread among swine (Lange et al 2009). Moreover, Vincent et al (2010) demonstrated an insufficient protection of North American pigs against H1N1 2009 by the immune response from previous exposure or vaccination (Vincent et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Isolamento e caracterização do vírus da influenza pandêmico H1N1 em suínos no Brasil

et al. 2011

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761Pesq. Vet. Bras. 31(9):761-767, setembro 2011 RESUMO.-[Isolamento e caracterização do vírus da influenza pandêmico H1N1 em suínos no Brasil.] A infecção causada pelo vírus Influenza A (IAV) é endêmica em suí-nos no mundo inteiro. O surgimento da pandemia de influenza humana pelo vírus A/H1N1 (pH1N1) em 2009 levantou dúvi-das sobre a ocorrência deste vírus em suínos no Brasil. Durante o desenvolvimento de um projeto de pesquisa do vírus de influenza suína em 2009-2010, na Embrapa Suínos e Aves (CNPSA), foi detectado em um rebanho de suínos em Santa Catarina, Brasil, um surto de influenza altamente transmissí-vel causado pelo subtipo viral H1N1. Este vírus causou uma doença leve em suínos em crescimento e em fêmeas adultas, sem mortalidade. Tres leitões clinicamente afetados foram eutanasiados. As lesões macroscópicas incluiam consolidação leve a moderada das áreas cranioventrais do pulmão. Microscopicamente, as lesões foram caracterizadas por bronquiolite necrosante obliterativa e pneumonia broncointersticial. A imunohistoquímica, utilizando um anticorpo monoclonal contra a nucleoproteína do vírus influenza A, revelou marcação positiva no núcleo das células epiteliais bronquiolares. O tecido pulmonar de três leitões e os suabes nasais de cinco fêmeas e quatro leitões foram positivos para influenza A pela RT-PCR. O vírus influenza foi isolado de um pulmão, mais tarde sendo confirmado pelo teste de hemaglutinação (título HA 1:128) e por RT-PCR. A análise das seqüências de nucleotídeos dos genes da hemaglutinina (HA) e proteína da matriz (M) revelou que o vírus isolado foi consistente com o vírus pandêmico A/H1N1/ 2009 que circulou em humanos no mesmo período. Este é o primeiro relato de um surto de influenza causado pelo vírus pandêmico A/H1N1 em suínos no Brasil.

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“…Several experiments have demonstrated the susceptibility of pigs to H1N1pdm09, which resulted in an highly efficient spread among herds with presentation of clinical signs and lesions consistent with IAV infection (Lange et al, 2009;Pasma and Joseph, 2010;Pereda et al, 2010;Vincent et al, 2010).…”

Section: Influenza a Virus In Swinementioning

confidence: 99%

Influenza Virus Infection in Pigs

Zanella¹

2013

VR&R

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Infl uenza A virus is a zoonotic agent of great relevance to human and animal health. Swine is an important host to the dynamics and epidemiology of the infection due to its susceptibility to viruses of both the avian and mammalian virus lineages. Th e great genetic variability of infl uenza viruses is caused by two main genetic mechanisms: point mutations and gene reassortment. Th e latter, by letting the exchange of gene segments between two diff erent infl uenza viruses infecting the same cell, allows a rapid evolution of infl uenza viruses and the emergence of reassortant viruses against which there is no immunity in the host (human or animal). Infl uenza is endemic in pigs in many countries and the emergence of new viruses has been challenging its control and diagnostics. Although infections with infl uenza A virus (IAV) are endemic in most of the pork producing countries throughout the world, in Brazil this pathogen has not received much attention. Since the emergence of the 2009 pandemic H1N1 infl uenza virus in pigs (H1N1pdm09), many outbreaks of respiratory disease were observed in Brazilian swine populations. Currently, in Brazil, H1N1, H3N2 and 2009 pandemic H1N1 (A(H1N1)pdm09) infl uenza A viruses (IAVs) circulate in domestic swine herds.

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Efficacy of inactivated swine influenza virus vaccines against the 2009 A/H1N1 influenza virus in pigs

Cited by 86 publications

References 21 publications

Modifications in the Polymerase Genes of a Swine-Like Triple-Reassortant Influenza Virus To Generate Live Attenuated Vaccines against 2009 Pandemic H1N1 Viruses

Modifications in the Polymerase Genes of a Swine-Like Triple-Reassortant Influenza Virus To Generate Live Attenuated Vaccines against 2009 Pandemic H1N1 Viruses

Isolamento e caracterização do vírus da influenza pandêmico H1N1 em suínos no Brasil

Influenza Virus Infection in Pigs

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