Efficacy of Large Groove Texture on Rat Sciatic Nerve Regeneration In Vivo Using Polyacrylonitrile Nerve Conduits

Wang, Zonghuan; Wu, Yibing; Xiang, Yang; Kruth, Marie Beatrix; Wei, Peng; Dai, Guangli; Xu, Kedi; Yin, Jun; Huang, Yong

doi:10.1007/s10439-020-02560-7

Cited by 18 publications

(14 citation statements)

References 50 publications

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“…In the case of width of 10–20 μm, axons can grow in an improper manner with a reduction in NCs guidance function. NCs with higher inner microchannel width (200–300 μm) were distinguished for an effective guiding role towards axons, vascular cells and glial cells [ 27 ]; the same evidence was also displayed by Wang and collaborators [ 26 ] adopting the same topographies in hollow tubes with patterned inner walls. Here, a high width alligned pattern (300–400 μm) was used with encouaging results.…”

Section: Discussionmentioning

confidence: 74%

“…Many groove topographies exist and the identification of the most effective one can present a challenge; however, there is a consensus that conduits with longitudinally aligned inner textures can favorably modulate neuronal cell behavior. Additionally, a patterned groove also increases the surface-area-to-volume-ratio, promoting cells–scaffolds interactions, and ameliorating the microenvironment within the lumen because of an increased area for the dynamic diffusion of nutrients/waste products [ 26 ]. Another topic of discussion regards groove width.…”

Section: Discussionmentioning

confidence: 99%

“…Another topic of discussion regards groove width. A 10–20 µm distance has been applied by many authors; however, it should be noticed that hierarchical nerve fascicles have a higher size (100 to 1000 μm) [ 23 , 26 ]. In the case of width of 10–20 μm, axons can grow in an improper manner with a reduction in NCs guidance function.…”

Section: Discussionmentioning

confidence: 99%

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Bioactivated Oxidized Polyvinyl Alcohol towards Next-Generation Nerve Conduits Development

et al. 2021

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The limitations and difficulties that nerve autografts create in normal nerve function recovery after injury is driving research towards using smart materials for next generation nerve conduits (NCs) setup. Here, the new polymer partially oxidized polyvinyl alcohol (OxPVA) was assayed to verify its future potential as a bioactivated platform for advanced/effective NCs. OxPVA-patterned scaffolds (obtained by a 3D-printed mold) with/without biochemical cues (peptide IKVAV covalently bound (OxPVA-IKVAV) or self-assembling peptide EAK (sequence: AEAEAKAKAEAEAKAK), mechanically incorporated (OxPVA+EAK) versus non-bioactivated scaffold (peptide-free OxPVA (PF-OxPVA) supports, OxPVA without IKVAV and OxPVA without EAK control scaffolds) were compared for their biological effect on neuronal SH-SY5Y cells. After cell seeding, adhesion/proliferation, mediated by (a) precise control over scaffolds surface ultrastructure; (b) functionalization efficacy guaranteed by bioactive cues (IKVAV/EAK), was investigated by MTT assay at 3, 7, 14 and 21 days. As shown by the results, the patterned groove alone stimulates colonization by cells; however, differences were observed when comparing the scaffold types over time. In the long period (21 days), patterned OxPVA+EAK scaffolds distinguished in bioactivity, assuring a significantly higher total cell amount than the other groups. Experimental evidence suggests patterned OxPVA-EAK potential for NCs device fabrication.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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Bioactivated Oxidized Polyvinyl Alcohol towards Next-Generation Nerve Conduits Development

et al. 2021

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“…provided a potential in vitro model to explore different dimensions of grooved conduits and evaluate the performances of different cells [ 68 ]. Meanwhile, NGCs with different sizes of grooved structures on the inner surface have also been applied for nerve repair in vivo [ 69 ]. The smaller ones, such as nanogrooves [ 70 ] and 3 μm stripe micropatterns on the inner wall of NGCs [ 71 ], were demonstrated to effectively facilitate sciatic nerve regeneration.…”

Section: Strategies For 3d Printing Functional Conduitsmentioning

confidence: 99%

3D printing of functional nerve guide conduits

Huang

Liu

et al. 2021

Burns &Amp; Trauma

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Nerve guide conduits (NGCs), as alternatives to nerve autografts and allografts, have been widely explored as an advanced tool for the treatment of peripheral nerve injury. However, the repairing efficiency of NGCs still needs significant improvements. Functional NGCs that provide a more favorable microenvironment for promoting axonal elongation and myelination are of great importance. In recent years, 3D printing technologies have been widely applied in the fabrication of customized and complex constructs, exhibiting great potential for tissue engineering applications, especially for the construction of functional NGCs. In this review, we introduce the 3D printing technologies for manufacturing functional NGCs, including inkjet printing, extrusion printing, stereolithography-based printing and indirect printing. Further, we summarize the current methods and strategies for constructing functional NGCs, such as designing special conduit architectures, using appropriate materials and co-printing with different biological cues. Finally, the challenges and prospects for construction of functional NGCs are also presented.

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“…However, the autografts still have many limitations, like donor repeated operations, nerve unavailability, local tissue adhesion, and size mismatch ( Daly et al, 2013 ; Tang et al, 2013 ; Jahromi et al, 2019 ). Recently, the development of tissue engineering has pointed a new direction on PNI treatment, i.e., nerve conduit ( Suo et al, 2018 ; Liu et al, 2021 ; Wang et al, 2021 ), which is an artificial hollow tube as an alternative that connects the two ends of the injured nerve to support nerve migration and regeneration.…”

Section: Introductionmentioning

confidence: 99%

Effect of Electrical and Electromechanical Stimulation on PC12 Cell Proliferation and Axon Outgrowth

Liu

et al. 2021

Front. Bioeng. Biotechnol.

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Peripheral nerve injuries have become a common clinical disease with poor prognosis and complicated treatments. The development of tissue engineering pointed a promising direction to produce nerve conduits for nerve regeneration. Electrical and mechanical stimulations have been incorporated with tissue engineering, since such external stimulations could promote nerve cell proliferation, migration and differentiation. However, the combination of electrical and mechanical stimulations (electromechanical stimulation) and its effects on neuron proliferation and axon outgrowth have been rarely investigated. Herein, silver nanowires (AgNWs) embedded polydimethylsiloxane (PDMS) electrodes were developed to study the effects of electromechanical stimulation on rat pheochromocytoma cells (PC12 cells) behaviors. AgNWs/PDMS electrodes demonstrated good biocompatibility and established a stable electric field during mechanical stretching. PC12 cells showed enhanced proliferation rate and axon outgrowth under electrical stimulation alone, and the cell number significantly increased with higher electrical stimulation intensity. The involvement of mechanical stretching in electrical stimulation reduced the cell proliferation rate and axon outgrowth, compared with the case of electrical stimulation alone. Interestingly, the cellular axons outgrowth was found to depend on the stretching direction, where the axons prefer to align perpendicularly to the stretch direction. These results suggested that AgNWs/PDMS electrodes provide an in vitro platform to investigate the effects of electromechanical stimulation on nerve cell behaviors and can be potentially used for nerve regeneration in the future.

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Efficacy of Large Groove Texture on Rat Sciatic Nerve Regeneration In Vivo Using Polyacrylonitrile Nerve Conduits

Cited by 18 publications

References 50 publications

Bioactivated Oxidized Polyvinyl Alcohol towards Next-Generation Nerve Conduits Development

Bioactivated Oxidized Polyvinyl Alcohol towards Next-Generation Nerve Conduits Development

3D printing of functional nerve guide conduits

Effect of Electrical and Electromechanical Stimulation on PC12 Cell Proliferation and Axon Outgrowth

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