2020
DOI: 10.3390/v12050559
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Efficacy of Lytic Phage Cocktails on Staphylococcus aureus and Pseudomonas aeruginosa in Mixed-Species Planktonic Cultures and Biofilms

Abstract: The efficacy of phages in multispecies infections has been poorly examined. The in vitro lytic efficacies of phage cocktails AB-SA01, AB-PA01, which target Staphylococcus aureus and Pseudomonas aeruginosa, respectively, and their combination against their hosts were evaluated in S. aureus and P. aeruginosa mixed-species planktonic and biofilm cultures. Green fluorescent protein (GFP)-labelled P. aeruginosa PAO1 and mCherry-labelled S. aureus KUB7 laboratory strains and clinical isolates were used as target bac… Show more

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Cited by 30 publications
(20 citation statements)
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“…For example, phage cocktails AB-SA01 and AB-PA01 which target S. aureus and P. aeruginosa , respectively, when combined together, significantly reduced biofilm biomass in mixed-species biofilms, compared to the respective phage cocktail treatment. 65 A mixture of two phages, philPLA-RODI and philPLA-C1C, showed a reduction in the amount of adhered bacterial cells to about 2 log units in both mono-species and dual-species biofilms of S. aureus and S. epidermidis . 66 These studies suggest that application of phage cocktails other than individual phages in biofilm models, is highly efficient at destroying bacterial biofilms.…”
Section: Application Of Phages In Bacterial Biofilm Destructionmentioning
confidence: 97%
“…For example, phage cocktails AB-SA01 and AB-PA01 which target S. aureus and P. aeruginosa , respectively, when combined together, significantly reduced biofilm biomass in mixed-species biofilms, compared to the respective phage cocktail treatment. 65 A mixture of two phages, philPLA-RODI and philPLA-C1C, showed a reduction in the amount of adhered bacterial cells to about 2 log units in both mono-species and dual-species biofilms of S. aureus and S. epidermidis . 66 These studies suggest that application of phage cocktails other than individual phages in biofilm models, is highly efficient at destroying bacterial biofilms.…”
Section: Application Of Phages In Bacterial Biofilm Destructionmentioning
confidence: 97%
“…Studies of in vitro phage-host interactions in liquid culture commonly use optical density to compare bacterial growth dynamics in response to different phage treatments [18][19][20][21][22][23]. Optical density measurements are quick and amenable to high-throughput comparison using 96-well incubating spectrophotometers, and hosts can even be fluorescently labeled to allow differentiation [24]. However, phage quantification in these cultures, especially those with phage mixtures, is frequently avoided due to the extra effort required and inability to quantitatively differentiate multiple phages.…”
Section: Introductionmentioning
confidence: 99%
“…Among these studies, one article targeted biofilm produced by P. aeruginosa , two by S. aureus , three by E. faecalis , and one by K. pneumoniae . One study tested the effect of phages on dual infections caused by S aureus and P. aeruginosa and one tested their effect on both organisms individually [ 34 , 43 ]. In all the reviewed studies, bacteriophage was active against both planktonic cells and biofilm, showing a bactericidal effect and detachment activities against tested pathogens.…”
Section: Resultsmentioning
confidence: 99%
“…In this study, although both bacteriophage and bacteriophage plus antibiotic combination reduced bacterial load in the animal, they did impact biofilm thickness. Another study compared the activity of phages vs. antibiotic (tetracycline) against S. aureus and P. aeruginosa dual infection biofilm where tetracycline showed superior anti-biofilm activity than the tested phages [ 34 ].…”
Section: Resultsmentioning
confidence: 99%