Efficacy of Methanolic Extract and its Fractions of some Tree Species for Potential in Vitro Antibacterial Activity

Aly, Hussein; Salem, Mohamed Z. M.; Gohar, Yousry M.; El-Sayed, Abdel-Wahab; Ashmawy, Nader A.

doi:10.24102/ijafr.v1i1.116

Cited by 2 publications

(1 citation statement)

References 39 publications

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“…The methanol (MeOH) extract from each part was subsequently partitioned using separating-funnel into ethyl acetate (EtOAc), n-butanol (n-BuOH) and water (Aq). These fractions were separated with solvent/solvent group separation procedure used by the USA National Cancer Institute separation procedure (Aly et al, 2012;Suffness and Douros, 1979).…”

Section: Preparation Of Extractsmentioning

confidence: 99%

Biological Activity of Extracts from Morus alba L., Albizzia lebbeck (L.) Benth. and Casuarina glauca Sieber Against the Growth of some Pathogenic Bacteria

Salem¹,

Aly²,

Gohar³

et al. 2013

International Journal of Agricultural and Food Research

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Different extracts from sapwood, heartwood and bark from Morus alba, Albizzia lebbeck and Casuarina glauca were used as an antibacterial agent against the growth of some pathogenic bacteria. Methanol (80%) was used for the extraction and ethyl acetate, n-butanol, chloroform and water were used for the subsequent partitioning. The in vitro antibacterial activity was performed by disc diffusion and 96-well micro-plates methods. The antibacterial activity was compared with standard antibiotics. The ethyl acetate fraction (EtOAc fr) of wood and bark exhibited the strongest antibacterial activity against the studied bacterial strains. The most active extracts were observed by M. alba. The extracts of A. lebbeck and C. glauca showed a weak to moderate activity against the tested bacterial strains. These results are of interest since they have been obtained with methanol extract and its fractions dissolved into different solvents, and are not a pure product. It could be considered that the extracts of M. alba, A. lebbeck and C. glauca have a good potency level of antibacterial activity. The obtained results might be considered sufficient for further studies about the isolation and identification of active principles compounds especially from C. glauca.

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Section: Preparation Of Extractsmentioning

confidence: 99%

Biological Activity of Extracts from Morus alba L., Albizzia lebbeck (L.) Benth. and Casuarina glauca Sieber Against the Growth of some Pathogenic Bacteria

Salem¹,

Aly²,

Gohar³

et al. 2013

International Journal of Agricultural and Food Research

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In vitro antimicrobial activity of Syzygium cumini fruit peel and identification of anthocyanins

Priya¹

2013

Afr. J. Pharm. Pharmacol.

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The present investigation evaluated the antimicrobial potential of Syzygium cumini (SC) fruit peel against fourteen microorganisms and identified major anthocyanins. Methanol, ethyl acetate and dichloromethane extracts of shade dried SC fruit peel were screened for antimicrobial effects using disc diffusion and minimum inhibitory concentration (MIC) methods. Methanol extract showed maximum antimicrobial potency against all the test microorganisms with inhibition zone diameter (IZD) ranging from 9.35±0.49 to 19.25±0.35 mm and the lowest MIC of 0.18 mg/ml. Ethyl acetate and dichloromethane extracts moderately inhibited the growth of microorganisms. Separation of anthocyanins from crude methanol extract was carried out by thin layer chromatography (TLC) and the compounds were isolated with Liquid Chromatography-Electrospray-Ionization Tandem Mass Spectrometry (LC-ESI-MS/MS). The compounds identified were malvidin, petunidin and cyanidin. Our results indicate the antimicrobial efficacy of SC fruit peel and subsequent identification of anthocyanins. However, further studies are required to explore anthocyanins in order to develop novel drug candidates.

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Efficacy of Methanolic Extract and its Fractions of some Tree Species for Potential in Vitro Antibacterial Activity

Cited by 2 publications

References 39 publications

Biological Activity of Extracts from Morus alba L., Albizzia lebbeck (L.) Benth. and Casuarina glauca Sieber Against the Growth of some Pathogenic Bacteria

Biological Activity of Extracts from Morus alba L., Albizzia lebbeck (L.) Benth. and Casuarina glauca Sieber Against the Growth of some Pathogenic Bacteria

In vitro antimicrobial activity of Syzygium cumini fruit peel and identification of anthocyanins

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