Efficacy of methuselah gene mutation toward tolerance of dichlorvos exposure in Drosophila melanogaster

Pandey, Ashutosh; Khatoon, Rehana; Saini, Sanjay; Vimal, Divya; Patel, Devendra Kumar; Narayan, Gopeshwar; Chowdhuri, D. Kar

doi:10.1016/j.freeradbiomed.2015.02.025

Cited by 14 publications

(11 citation statements)

References 55 publications

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“…Therefore, organismal responses may offer a rostrum for the assessment of tolerance/resistance of organisms against their exposure to xenobiotics. Organismal adversities (survival and locomotor activity) in different model organisms (rats, Xenopus, and zebrafish) are well reported after their chronic exposure to DDVP [3,8,40]. Heat shock genes are also reported to be induced in organisms after their exposure to xenobiotics [17,43].…”

Section: Discussionmentioning

confidence: 99%

“…[42]. A stock solution of DDVP (20.0 μg/ml) was prepared and further added to Drosophila food to achieve the final test concentration (15.0 ng/ml) which was based on a pervious study [40]. Along with the control group receiving standard Drosophila food, a vehicle control group receiving 0.3 % DMSO mixed food was used.…”

Section: Fly Strains and Culturementioning

confidence: 99%

“…As a measure of general cellular oxidative stress, level of reactive oxygen species was examined in terms of the oxidation of dihydroethidium (DHE) in brain tissues [40]. In brief, adult brain tissues from control and exposed flies were treated with collagenase (0.5 mg/ml) to prepare single cell suspension.…”

Section: Assessment Of Oxidative Stressmentioning

confidence: 99%

“…Locomotor performance is considered as a reliable parameter for the assessment of senescence of motor function in flies [37]. Further, this model has been used to comprehend the association between hsps and aging process [38] and to examine cellular as well organismal responses against long-term low-level xenobiotic exposure [39][40][41]. This invertebrate model raises fewer ethical concerns and falls within the recommendations of the European Centre for the Validation of Alternative Methods (ECVAM) and aims to hold the scientific and regulatory acceptance of alternative methods, which are important in the field of biological science and towards reducing, refining, and replacing the use of laboratory animals.…”

Section: Introductionmentioning

confidence: 99%

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Overexpression of hsp27 Rescued Neuronal Cell Death and Reduction in Life- and Health-Span in Drosophila melanogaster Against Prolonged Exposure to Dichlorvos

et al. 2015

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Long-term exposure to dichlorvos (O,O-dimethyl-2,2-dichlorovinyl phosphate (DDVP), an organophosphate pesticide) is reported to exert neurotoxicity, i.e., generation of reactive oxygen species (ROS), oxidative damage, and neuronal cell death along with life- and health-span reduction in nontarget organisms including humans. However, studies on genetic modulation towards neuroprotection against prolonged DDVP exposure are elusive. Hsp27 (a small heat shock protein) is involved in various cellular processes and thus has attained emphasis as a therapeutic target. We aimed to examine the protective effect of hsp27 overexpression against prolonged DDVP exposure using an in vivo model Drosophila melanogaster. Flies were exposed to 15.0 ng/ml DDVP for a prolonged period to examine neuronal cell death, locomotor performance, and lifespan. After prolonged exposure, cell death, ROS level, glutathione depletion, nicotinamide adenine dinucleotide phosphate level (NADPH), glucose-6-phosphate dehydrogenase (G6PD), and thioredoxin reductase (TrxR) activities were examined in fly brain tissues at different days of age (days 10, 20, and 30). Flies with ubiquitous overexpression of hsp27 showed better resistance (improved lifespan and locomotor performance) in comparison to that targeted to motor neurons and nervous system. These flies also exhibited lesser intracellular ROS level and glutathione depletion by restoring G6PD activity, NADPH level, and TrxR activity in their brains thereby resisted neuronal cell death. Conversely, hsp27 knockdown flies exhibited reversal of the above endpoints. The study evidenced the neuroprotective efficacy of hsp27 overexpression against prolonged DDVP exposure and favored Hsp27 as a therapeutic target towards achieving better organismal (including human) health against long-term chemical exposure.

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Section: Discussionmentioning

confidence: 99%

Section: Fly Strains and Culturementioning

confidence: 99%

Section: Assessment Of Oxidative Stressmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Overexpression of hsp27 Rescued Neuronal Cell Death and Reduction in Life- and Health-Span in Drosophila melanogaster Against Prolonged Exposure to Dichlorvos

et al. 2015

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“…Moreover, the availability of wide range of C. elegans mutants and transgenic models offers ease and reliability to study the ROSassociated disorders. Similarly, Drosophila melanogaster is highly sensitive to oxidative stress and severity can be easily probed by some physiological behavior and biochemical assays (Pandey et al 2015). Thus, we used mouse embryonic fibroblast cell line (3T3-L1), C. elegans (N2 wild type and CL4176 transgenic Alzheimer's disease model), and D. melanogaster (Oregon R + ) in this study.…”

mentioning

confidence: 99%

Phycoerythrin averts intracellular ROS generation and physiological functional decline in eukaryotes under oxidative stress

Sonani

Rastogi

Singh³

et al. 2016

Protoplasma

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In vitro antioxidant virtue and life-prolonging effect of phycoerythrin (PE; a pigment protein isolated from Phormidium sp. A09DM) have been revealed in our previous reports (Sonani et al. in Age 36:9717, 2014a; Sonani et al. in Process Biochem 49:1757-1766, 2014b). It has been hypothesized that the PE expands life span of Caenorhabditis elegans (bears large resemblance with human aging pathways) due to its antioxidant virtue. This hypothesis is tested in present study by checking the effect of PE on intracellular reactive oxygen species (ROS) generation and associated physiological deformities using mouse and human skin fibroblasts, C. elegans, and Drosophila melanogaster Oregon R and by divulging PE's structural attributes responsible for its antioxidant asset. PE treatment displayed noteworthy decrease of 67, 48, and 77 % in ROS level in mouse fibroblast (3T3-L1), human fibroblast, and C. elegans N2, respectively, arisen under chemical-induced oxidative stress. PE treatment delayed the development of paraquat-induced Alzheimer phenotype by 14.5 % in C. elegans CL4176. Furthermore, PE improved the locomotion of D. melanogaster Oregon R under oxidative stress with simultaneous up-regulation in super-oxide dismutase and catalase activities. The existence of 52 Glu + Asp + His + Thr residues (having metal ion sequestration capacity), 5 phycoerythrobilin chromophores (potential electron exchangers) in PE's primary structure, and significant hydrophobic patches on the surface of its α- and β-subunits are supposed to collectively contribute in the antioxidant virtues of PE. Altogether, results support the hypothesis that it is the PE's antioxidant asset, which is responsible for its life-prolonging effect and thus could be exploited in the therapeutics of ROS-associated abnormalities including aging and neurodegeneration in eukaryotes.

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Cr(VI)‐induced DNA damage is lessened by the modulation of hsp70 via increased GSH de novo synthesis in Drosophila melanogaster

Chaouhan

Jha

Patel

et al. 2021

J Biochem & Molecular Tox

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Hexavalent chromium [Cr(VI)] is a genotoxic chemical, and in the chemical‐exposed organism, oxidative stress is one of the leading causative mechanisms of genotoxicity. Heat shock protein‐70 (Hsp70) is reported to be modulated in environmental chemical exposed organisms. Inadequate information on the protective role of Hsp70 in chemical‐induced DNA lesions prompted us to investigate this possibility in a well‐studied genetically tractable in vivo model Drosophila melanogaster. In the midgut cells of Cr(VI)‐exposed hsp70‐knockout (KO), ‐knockdown (KD), and ‐overexpression Drosophila strains, no significant change in double‐strand breaks generation was observed in comparison to similarly exposed w 1118 and the respective genetic control strain after 48 h. Therefore, the role of hsp70 was investigated on oxidative DNA damage induction in the exposed organisms after 24 h. Oxidized DNA lesions (particularly oxidized purine‐based lesions), 8‐oxo‐dG level, and oxidative stress endpoints were found to be significantly elevated in hsp70‐KO and ‐KD strains in comparison to similarly exposed w 1118 and respective genetic control strain. On the contrary, in ubiquitous hsp70‐overexpression strain exposed to Cr(VI), these endpoints were significantly lowered concurrently with increased GSH level through elevated gclc, and gclm expression, Gclc level, and GCL activity. The study suggests that as a consequence of hsp70 overexpression, the augmented GSH level in cells vis‐a‐vis GSH de novo synthesis can counteract Cr(VI)‐induced oxidized DNA lesions.

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Efficacy of methuselah gene mutation toward tolerance of dichlorvos exposure in Drosophila melanogaster

Cited by 14 publications

References 55 publications

Overexpression of hsp27 Rescued Neuronal Cell Death and Reduction in Life- and Health-Span in Drosophila melanogaster Against Prolonged Exposure to Dichlorvos

Overexpression of hsp27 Rescued Neuronal Cell Death and Reduction in Life- and Health-Span in Drosophila melanogaster Against Prolonged Exposure to Dichlorvos

Phycoerythrin averts intracellular ROS generation and physiological functional decline in eukaryotes under oxidative stress

Cr(VI)‐induced DNA damage is lessened by the modulation of hsp70 via increased GSH de novo synthesis in Drosophila melanogaster

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