Efficacy of Nano Composite Porous 3D Scaffold of Crab Shell and Al-Kharit Histological and Radiological for Bone Repair in Vivo

Zebon, Saad H.

doi:10.30539/ijvm.v44i2.973

Cited by 6 publications

(6 citation statements)

References 29 publications

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“…The level of serum alkaline phosphatase in both groups at 7-and 14-days post-surgery was significantly increased. These results are exhibited through the increased osteoblastic activity when osteoblast secretes large quantities of alkaline phosphatase that get involved in process of mineralization and bone defect formation as a result of adding the hydroxyapatite nano gel as a biomaterial, these findings agreed with previous works Thanoon et al (23) and Zebon et al (24). The tissue sections from the xenograft group 15 days post-surgery showed the presence of granulation tissue with inflammatory reaction without bone formation at the site of bone defect this occurs as a result of secretion of inflammatory cytokines such as vascular endothelial growth factor (VEGF) transforming growth factor beta (TGFB) fibroblast growth factor 2 (FGF2) and angiopoietin (25).…”

Section: Discussionsupporting

confidence: 91%

Histopathological and serological assessment of using rib lamb xenograft reinforced with and without hydroxyapatite nano gel for reconstruction tibial bone defect in dogs

Albaroudy¹,

Alkattan²,

Ismail³

2022

IJVS

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This work aimed to assess repairing tibial bone defects using histopathological and serological examinations. Eighteen stray adult dogs of both sexes were used. The experiment was allocated into two equal group s, 9 of each. An experimental tibia defect of 2.5*0.7 cm was induced at the proxomedial aspect of the tibia. Deproteinized lamb's rib xenograft was used to reconstitute the defective area. The tissue and blood samples were collected for histopathological and serological investigations at different periods. On day seven post-surgery, the serological assessment indicated a significant increase in the level of Insulin-Like Growth Factor (IGF) of both the first and second groups, 0.2±0.03, 0.3±0.02 ng/ml, respectively; however, after 14 days, the levels were significantly reduced in both groups 0.08±0.03, 0.2±0.02 ng/ml, respectively. On day 7, the serum alkaline phosphatase level in the first group was lower, 31.6±3 u/l, than in the treated group, 54.2±1.86 u/l. However, at 14 days post-surgery, the serum alkaline phosphatase level in the first group slightly increased by 35.7±2.1 u/l; nevertheless, the treated group manifested a constant level of 54.1±5.24 u/l. At 60 days post-operation, the histopathological examination presented more organized tissue maturation in the second group. The histochemical results of all specimens of the hydroxyapatite group revealed an increase in calcified bone by showing a red reaction which indicates the formation of thick calcified compact bone at 60 days post defects. In conclusion, the hydroxyapatite Nano gel contributed to ossification across the bone defect and hastened the healing process; the serological investigations indicated an increase in the activity of bone tissue.

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Section: Discussionsupporting

confidence: 91%

Histopathological and serological assessment of using rib lamb xenograft reinforced with and without hydroxyapatite nano gel for reconstruction tibial bone defect in dogs

Albaroudy¹,

Alkattan²,

Ismail³

2022

IJVS

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“…In this study, we used hydroxyapatite Nano gel with high viscosity (33%), which was prepared by strict sterilization and proceeded firmly and homogenously spread along the edge of repaired bone defect, and this preparation proceeds as gel form could improve the practical utility of Hydroxyapatite this finding pointed by manyinvestigators [5,28]. The fast mineralization of bone and acceleration in the healing process in the treatment group this due to the role of host blood vessels which makes a network easy to transport the osteoblastic cells to the site of defect this may be related to the osteoconductive properties of hydroxyapatite nano gel and this material are biodegradable, biocompatible with no any immune reaction [18,29]. Radiographic findings exhibited good knowledge about animals status.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of The Role of Hydroxyapatite Nano Gel as Filling Materials for Improving The Healing of Repaired Tibial Bone Defect In Dogs

Alkattan

2023

Egyptian Journal of Veterinary Sciences

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A LARGE size bone defect is a challenging problem in orthopedic. This trial focuses on investigating the effects of Hydroxyapatite Nano gel as filling materials for accelerating the healing of repaired tibial bone defects in dogs. The experiment included 18 healthy dogs which was randomized divided into two equal groups, 9 of each. The experimental bone defect was created at (2.5 by 0.7cm ) in both groups. In the control group, the same bone piece is reimplanted at the experiment defect and fixed with Cerclage wire. In the second group, a deproteinized lamb rib at (2.4 by 0.6cm) was used to repair the bone defect, and the graft segment was fixed with Cerclage wire, and the repaired defect was supported with hydroxyapatite Nano gel. The animals were monitored clinically, grossly and radiologically on days 14, 30 and 60. The defect completely healed with no apparent changes on day 60 post-operatively. Radiographically, in the control group on day 60, there was a faint lucent line around the fixated bone segment with minimal cortical thickening and irregularities denoting late chronic periosteal reaction and callus formation and near complete healing with the surrounding area whereas in the treatment group, the callus formation was superior in comparison with control group and there are some cortical irregularities denoting chronic periosteal reaction. We demonstrate that using hydroxyapatite nano gel as an additive to the defective bone fills the voids, accelerates the healing process. Radiological analysis showed superior healing and callus formation in hydroxyapatite nano gel group with xenobone graft.

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“…Changes in dosing from PGF2 (24) to Nano-form have been shown to increase both the efficiency and duration of action by virtue of the Nano-size-dependent, form's increased and intensified activity structure Nano-Lipid (25,26). This may have occurred because PGF2 reconcentrates in the utero-ovarian artery counter-current vein, thereby altering the effect of luteolysis and adjusting ovarian luteal regression.…”

Section: Discussionmentioning

confidence: 99%

Effects of Nano-Lipid Prostaglandin F2α on Synchronization

Abdullaha

Al-Bayati²

2023

The Egyptian Journal of Hospital Medicine

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Background: By modulating the estrous cycle, numerous oestrus-inducing or synchronising therapeutic hormones and medicines are accessible for pharmacological use, allowing for rapid onset of estrus in the majority of animals. Objective: The purpose of this research is to evaluate the efficacy of Nano-Lipid containing prostaglandin F2 nanotechnology in synchronising the estrous cycle. Material and Methods: The strategy to induce and synchronise estrus75 in 4-to 8-week-old female mice was tested in an animal investigation to establish its efficacy across three treatment groups: control, conventional, and SNLPGF2. The first group received a saline injection as a control, the second group received conventional hormone injections at 5, 10, 15, 20, and 25 IU g/kg body weight (I.P. ), and the third group received SNL PGF2 hormone injections at 5, 10, 15, 20, and 25 IU g/kg body weight I.M. Results: Nano-lipid Size The PGF2 ranged in length from 50 to 166.6 nm. whereas SNL PgF2 trapping efficiency is 84.11 (SD 5.55). As expected, autolysis and a rise in the estrus phase and an increase in the amount of estrogen were responsible for the greatest degree of synchronisation in the group that received large doses of nano-treatment. Conclusion: results of this research demonstrate that Prostaglandin F2 f Nano-lipid has a high efficiency for synchronising the estrous cycle..

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Efficacy of Nano Composite Porous 3D Scaffold of Crab Shell and Al-Kharit Histological and Radiological for Bone Repair in Vivo

Cited by 6 publications

References 29 publications

Histopathological and serological assessment of using rib lamb xenograft reinforced with and without hydroxyapatite nano gel for reconstruction tibial bone defect in dogs

Histopathological and serological assessment of using rib lamb xenograft reinforced with and without hydroxyapatite nano gel for reconstruction tibial bone defect in dogs

Evaluation of The Role of Hydroxyapatite Nano Gel as Filling Materials for Improving The Healing of Repaired Tibial Bone Defect In Dogs

Effects of Nano-Lipid Prostaglandin F2α on Synchronization

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