Efficacy of Nasopharyngeal Culture in Identification of Pathogen in Middle Ear Fluid in Chronic Otitis Media With Effusion

Eser, Özgen Köseoğlu; Ipçi, Kaan; Alp, Şehnaz; Akyol, Umut; Ünal, Ömer Faruk; Hasçelik, Gülşen; Sennaroḡlu, Levent; Gür, Deniz

doi:10.4103/0255-0857.53206

Cited by 13 publications

(11 citation statements)

References 22 publications

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“…Some studies have suggested a good agreement for serotypes and susceptibility patterns of microorganisms found in paired NP and MEF samples, suggesting that NP sampling may be of value for the monitoring of antimicrobial resistance in children with OM. 10,12,23 Last, only 4 studies used polymerase chain reaction: these detected more microorganisms in AOM and OME than conventional methods except for the study by Eser et al 11 None used newer molecular techniques that could even provide higher accuracy in testing for microbial presence. 34,35 For a better understanding of the etiology of OM and the relation between the microflora in the nasopharynx and the middle ear, more research into microbial interactions and pathogen-host interactions is needed using more advanced diagnostic techniques.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Concordance Between the Microorganisms Detected in the Nasopharynx and Middle Ear of Children With Otitis Media

Dongen

Heijden²,

Zon³

et al. 2013

Pediatric Infectious Disease Journal

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Studies of microorganisms involved in otitis media in children often use a nasopharyngeal sample as a proxy for the middle ear fluid to test for bacteria and viruses. The question is whether such studies provide an accurate estimate of the prevalence of microorganisms involved in otitis media. We performed a systematic review of the literature reporting on the concordance between test results of nasopharyngeal and middle ear fluid samples for the most prevalent microorganisms in children with otitis media. Our findings show that the concordances vary from 68% to 97% per microorganism. For the most prevalent microbes, positive predictive values are around 50%. Most negative predictive values are moderate to high, with a range from 68% up to 97%. These results indicate that test results from nasopharyngeal samples do not always provide an accurate proxy for those of the middle ear fluid. It is important to interpret and use results of such studies carefully.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Concordance Between the Microorganisms Detected in the Nasopharynx and Middle Ear of Children With Otitis Media

Dongen

Heijden²,

Zon³

et al. 2013

Pediatric Infectious Disease Journal

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“…Thus, it can result in developmental impairment of linguistic, behavioral, motor and social skills (1, 11, 12). …”

Section: Discussionmentioning

confidence: 99%

Concurrent Assay for Four Bacterial Species IncludingAlloiococcus Otitidisin Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report

Aydın

Taştan

Yücel

et al. 2012

Clin Exp Otorhinolaryngol

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Objectives To detect the prevalences of Alloiococcus otitidis , as well as Haemophilus influenzae , Streptococcus pneumoniae , and Moraxella catarrhalis in children with chronic otitis media with effusion (OME) and to simultaneously investigate the colonization of these bacteria in the nasopharynx and palatine tonsils of these patients. Methods The study included 34 pediatric patients with OME, and 15 controls without OME. In the study group, A. otitidis , H. influenzae , S. pneumoniae , and M. catarrhalis were investigated in the samples obtained from middle ear effusions (MEE), nasopharyngeal swabs (NPS) and tonsillar swabs (TS), using multiplex polymerase chain reaction (PCR) and conventional culture methods. Only the samples obtained from NPS and TS were studied with the same techniques in the control group. Results A. otitidis was isolated only in MEE and only with multiplex PCR method. A. otitidis , S. pneumoniae , M. catarrhalis , H. influenzae were identified in 35%, 8.8%, 8.8%, and 2.9%, respectively, in 34 MEE. A. otitidis was not isolated in NPS or TS of the study and the control groups. Conclusion The prevalence of A.otitidis is high in children with OME and A.otitidis doesn't colonize in the nasopharynx or tonsil.

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“…In our study the conventional culture and API identification revealed 30 CNS species (30.9%), with S. epidermidis as the leading species of CNS in the tested groups of patients. The detection rate of middle-ear pathogens in chronic infections, with conventional culture methods, varies between 2 and 20% for bacterial pathogens [13,17]. In many cases, is not satisfactorily in chronic OME.…”

Section: Discussionmentioning

confidence: 99%

The phenotypic and genetic biofilm formation characteristics of coagulase-negative staphylococci isolates in children with otitis media

Paluch-Oleś¹,

Magryś²,

Kozioł-Montewka³

et al. 2011

International Journal of Pediatric Otorhinolaryngology

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Efficacy of Nasopharyngeal Culture in Identification of Pathogen in Middle Ear Fluid in Chronic Otitis Media With Effusion

Abstract: PCR is a more reliable method to detect middle ear pathogens in MEF in comparison with the conventional culture methods. The NP colonization wasn't found to be an indicator of the pathogen in MEF although middle ear pathogens colonize more in nasopharynx of diseased children.

Cited by 13 publications

References 22 publications

Evaluation of Concordance Between the Microorganisms Detected in the Nasopharynx and Middle Ear of Children With Otitis Media

Evaluation of Concordance Between the Microorganisms Detected in the Nasopharynx and Middle Ear of Children With Otitis Media

Concurrent Assay for Four Bacterial Species IncludingAlloiococcus Otitidisin Middle Ear, Nasopharynx and Tonsils of Children with Otitis Media with Effusion: A Preliminary Report

The phenotypic and genetic biofilm formation characteristics of coagulase-negative staphylococci isolates in children with otitis media

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