Efficacy of native antagonistic bacterial isolates in biological control of crown gall disease in Egypt

Tolba, I. H.; Soliman, M A

doi:10.1016/j.aoas.2013.01.007

Cited by 30 publications

(12 citation statements)

References 30 publications

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“…Another solution relies on partnering cultureindependent studies with culture-dependent ones, i.e., community analysis and characterization of isolates, where dominant or differential bacteria can be selectively isolated for functional verification in vitro (37,41,42). Some culturable strains of Rhizobium, Pseudomonas, Bacillus, and Pantoea are successful biocontrol agents (43,44) or have high biological control potential against crown gall disease (30,(45)(46)(47)(48)(49)(50)(51)(52). In this study, similar genera were found to be strongly associated with pathogen invasion in a resistant peach cultivar, and some strains showed antagonistic activity via in vitro tests (see Fig.…”

Section: Discussionmentioning

confidence: 99%

Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance

Guo

et al. 2019

Appl Environ Microbiol

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Crown gall disease caused by Agrobacterium tumefaciens severely impacts the production of peach and other fruit trees. Several peach cultivars are partially resistant to A. tumefaciens, but little is known about the roles of endophytic microbiota in disease resistance. In the present study, the endophytic bacterial communities of resistant and susceptible peach cultivars "Honggengansutao" and "Okinawa" were analyzed using universal 16S rRNA gene amplicon sequencing in parallel with the cultivation and characterization of bacterial isolates. A total of 1,357,088 high-quality sequences representing 3,160 distinct operational taxonomic units (OTUs; Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes) and 1,200 isolates of 20 genera and 305 distinct ribotypes were collected from peach roots and twigs. It was found that factors including plant developmental stage, cultivar, and A. tumefaciens invasion strongly influenced the peach endophytic communities. The community diversity of endophytic bacteria and the abundance of culturable bacteria were both higher in the roots of the resistant cultivar, particularly after inoculation. Strikingly, the pathogen antagonists Streptomyces and Pseudomonas in roots and Rhizobium in twigs were most frequently detected in resistant plants. Our results suggest that the higher abundance and diversity of endophytic bacteria and increased proportions of antagonistic bacteria might contribute to the natural defense of the resistant cultivar against A. tumefaciens. This work reveals the relationships between endophytic bacteria and disease resistance in peach plants and provides important information for microbiome-based biocontrol of crown gall disease in fruit trees. IMPORTANCE Agrobacterium tumefaciens as the causal agent of peach crown gall disease can be controlled by planting resistant cultivars. This study profiles the endophytic bacteria in susceptible and resistant peach cultivars, advancing our understanding of the relationships between endophytic bacterial communities and peach crown gall disease, with potential implications for other complex microbiome-plantpathogen interactions. The resistant cultivar may defend itself by increasing the diversity and abundance of beneficial endophytic bacteria. The antagonists identified among the genera Streptomyces, Pseudomonas, and Rhizobium may have application potential for biocontrol of crown gall disease in fruit trees.

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Section: Discussionmentioning

confidence: 99%

Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance

Guo

et al. 2019

Appl Environ Microbiol

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“…Crown gall disease caused by Agrobacterium tumefaciens is a major bacterial disease in nurseries and orchards and is being considered one of the most important disorders that causes high losses in nurseries (Pulawska 2010). Biological control of crown gall is more effective in controlling the disease rather than chemical control (Tolba and Soliman 2013). Root-knot nematodes, Meloidogyne spp., are among the most important plant fauna that limit the productivity of many economic crops.…”

Section: Introductionmentioning

confidence: 99%

Influence of hydrogen cyanide-producing rhizobacteria in controlling the crown gall and root-knot nematode, Meloidogyne incognita

El-Rahman

Shaheen

El-Aziz

et al. 2019

Egypt J Biol Pest Control

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Out of 39 isolates of rhizobacteria, recovered from economic plants grown in 8 locations in Egypt, 6 isolates were able to produce Hydrogen Cyanide (HCN). 16S rRNA sequence analysis identified these isolates as: Pseudomonas japonica strain NBRC 103040, Bacillus megaterium strain CtST3.5, Pseudomonas sp. strain Gamma-81, P. tolaasii strain ATCC 33618, P. chlororaphis strain Lzh-T5, and P. mosselii strain CV25. These HCN producers were able to inhibit growth of Agrobacterium tumefaciens and affect viability of Meloidogyne incognita juveniles in vitro. The isolates of P. japonica and Pseudomonas sp. Gamma-81 prevented the gall formation on tomato plants by A. tumefaciens, regardless of the presence of M. incognita. The isolates of B. megaterium, P. chlororaphis, P. tolaasii, and P. mosselii decreased the weight and number of galls produced by A. tumefaciens in the presence or absence of M. incognita. The 6 HCN producers decreased the population of M. incognita and the number of nematode galls than the positive control, when used against M. incognita. A similar effect was achieved against mixed infections with M. incognita and A. tumefaciens. The HCN-producing rhizobacteria, in the presence of A. tumefaciens and/or M. incognita, caused obvious increment in all growth parameters of tomato than the negative control and healthy plants. The only exception was found in case of Pseudomonas sp. Gamma-81 against M. incognita and against mixed infection, where growth parameters of tomato were decreased. Although the isolates were naturally isolated from the rhizosphere of economic plants, it must be cautiously considered since the isolate identified as P. japonica has been reported as a human pathogen. Also, P. tolaasii was reported causing a bacterial blotch on cultivated mushrooms under certain environmental conditions. Further investigations are needed.

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“…The failed biocontrol of tumour formation by these Agrobacterium strains emphasises the need for alternative antagonistic bacteria, and indeed, efforts have been made to identify new strains. Bacterial genera studied for the control of crown gall disease include Agrobacterium , Pseudomonas , Serratia and Bacillus …”

Section: Introductionmentioning

confidence: 99%

Lipopeptides from a novel Bacillus methylotrophicus 39b strain suppress Agrobacterium crown gall tumours on tomato plants

Frikha‐Gargouri

Abdallah

Ghorbel

et al. 2016

Pest Management Science

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Efficacy of native antagonistic bacterial isolates in biological control of crown gall disease in Egypt

Cited by 30 publications

References 30 publications

Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance

Insight into the Bacterial Endophytic Communities of Peach Cultivars Related to Crown Gall Disease Resistance

Influence of hydrogen cyanide-producing rhizobacteria in controlling the crown gall and root-knot nematode, Meloidogyne incognita

Lipopeptides from a novel Bacillus methylotrophicus 39b strain suppress Agrobacterium crown gall tumours on tomato plants

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