Efficacy of new EC formulation derived from garlic creeper (Adenocalymma alliaceum Miers.) against anthracnose and stem end rot diseases of mango

Abstract

Different leaf extracts of Garlic creeper (Adenocalymma alliaceum Miers.) using water and solvents were prepared and they were screened for their antifungal activity against Colletotricum gloeosporioides Penz. and Botryodiplodia theobromae Pat. causal agents of mango post harvest diseases viz., anthracnose and stem end rot respectively. Among the extracts tested, chloroform extract was found to be highly effective in inhibiting the spore germination of C. gloeosporioides by 84.62% and B. theobromae by 84.50% followed by methanol extract. The extracts were also inhibitory to the mycelium. Ammonium sulphate precipitation and SDS-PAGE analysis of the extract indicated the presence of a major protein with a molecular weight at 65 kDa. Two blue spots at Rf 0.96 and 0.80 was also observed in TLC analysis and the presence of tannic acid, resorcinol was evident from HPLC analysis. Treatment of fruits with leaf extract of A. alliaceum increased the activities of peroxidase, polyphenol oxidase, phenylalanine ammonialyase and sugars with significant reduction in starch, phenolics, protein and ascorbic acid. The extract was partially purified and formulated as ADENOCAL 60 EC for the management of post harvest diseases of mango fruits.

Preparation and antimicrobial activity of thyme essential oil microcapsules prepared with gum arabic

“…However, thyme essential oil has not been studied for use as an additive to fruit preservation due to its strong odor, thermal instability and low ash point. 56,57 These problems can be solved by embedding thyme essential oil with b-cyclodextrin.…”

Factors influencing the incidence of basal stem rot and blight disease caused by Sclerotium rolfsii in vegetable cowpea and its management using botanicals

“…The methanolic extract of garlic creeper at 2.5 and 10.0 per cent inhibited the growth of Pythium aphanidermatum and Macrophomina phaseolina respectively (Girijashankar and Thayumanavan, 2005). Chloroform extracts of the leaves of garlic creeper were highly effective in inhibiting the mycelial growth and spore germination of Colletotrichum gloeosporioides (anthracnose) and Botrytis theobromae (stem end rot) in mango (Aswini et al, 2010). They also reported that thin layer chromatography of the leaf extracts revealed the presence of phenolic compounds viz., tannic acid and resorcinol, which had antimicrobial activity by reacting on sulph hydryl groups of pathogen enzymes.…”

Garlic the Wonder Adjuvant in Medicinal Field

“…Garlic powder is an herbal formulation used in ayurvedic system of medicine for the treatment of platelets aggregation: the interaction of garlic with heparin has been studied in influence to platelets aggregation effect of standard drug in various parameters. No sign toxicity was observed during toxicity study (5). The effects of garlic on the osmotic fragility of red blood cells in albino rats were assessed in vivo and in vitro.…”