Two indigenous isolates of Trichoderma isolated from rhizosphere soils of maize plants were identified as T. harzianum and assessed for antagonistic activity against Bipolaris maydis causing maydis leaf blight in fodder maizein vitro,and under field conditions for two consecutive kharif seasons of 2016 and 2017. Dual culture assay of T. harzianum isolates showed significantly higher degree of mycelial inhibition (74.35%) againstB. maydis. Similarly, under field conditions, seed treatment andfoliar spray of T. harzianum-I provided highest reduction in leaf blight severity (54.86%) along with 19.03 percent increase in green fodder yieldin comparison to control. The efficacy of T. harzianum isolates to boost defence responses against maydis leaf blight disease in maize was also evaluated in bioagents treated leaves. Plants treated with biocontrol agents showed significantly higher activities of antioxidative defence enzymes like peroxidase (POX), superoxide dismutase (SOD) and catalase (CAT). In treated leaves, the activities of POX and SOD reached maximum at 24 h and activity of CAT reached the highest at 36 h after inoculation of pathogen B. maydis. Enzyme activities induced by T. harzianum isolates were more obvious than that induced by pathogen only. This implies that biocontrol agent induced defence responses against maydis leaf blight pathogen in fodder maize.