Efficacy of novel phages for control of multi-drug resistant Escherichia coli O177 on artificially contaminated beef and their potential to disrupt biofilm formation

Montso, Peter Kotsoana; Mlambo, Victor; Ateba, Collins Njie

doi:10.1016/j.fm.2020.103647

Cited by 34 publications

(17 citation statements)

References 34 publications

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“…Interestingly, the individual phages showed reductions equal or greater than the cocktail in most treatments. Similar results were reported by Montso et al [21], who reported that the individual phages obtained greater efficacy than the cocktails after 1 day of treatment; nevertheless, the authors observed that after 7 days, there was bacterial regrowth in the treatments with individual phages but not in the cocktails. In addition to being effective at preventing bacterial growth, phage cocktails can be used to overcome the generation of phage resistance and to expand the range of bactericidal action [34,35].…”

Section: Discussionsupporting

confidence: 89%

“…Onmaz et al [19] reported that 24% of E. coli strains isolated were EPS producers, and 36% showed at least weak adhesion ability. The majority of studies were based solely on using the adhesion ability to classify the strains as biofilm-forming [6,[20][21][22]; however, we recommend performing both tests to have a better characterization of the main factors that lead the strains to produce biofilms. These studies reported results ranging from 11% to 100% of strains that showed some adhesion ability, suggesting that this characteristic is highly variable within the same species, which may be due to differences in flagellar motility, which helps the bacteria to counteract the electrostatic and hydrodynamic forces near surfaces, or it may depend on the type of surface because adhesion to abiotic surfaces is mediated by electrostatic and physicochemical interactions among the bacterial membrane, the surfaces, and the medium to which they are exposed [23,24].…”

Section: Discussionmentioning

confidence: 99%

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Efficacy of Novel Bacteriophages against Escherichia coli Biofilms on Stainless Steel

et al. 2021

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Biofilm formation by E. coli is a serious threat to meat processing plants. Chemical disinfectants often fail to eliminate biofilms; thus, bacteriophages are a promising alternative to solve this problem, since they are widely distributed, environmentally friendly, and nontoxic to humans. In this study, the biofilm formation of 10 E. coli strains isolated from the meat industry and E. coli ATCC BAA-1430 and ATCC 11303 were evaluated. Three strains, isolated from the meat contact surfaces, showed adhesion ability and produced extracellular polymeric substances. Biofilms of these three strains were developed onto stainless steel (SS) surfaces and enumerated at 2, 12, 24, 48, and 120 h, and were visualized by scanning electron microscopy. Subsequently, three bacteriophages showing podovirus morphology were isolated from ground beef and poultry liver samples, which showed lytic activity against the abovementioned biofilm-forming strains. SS surfaces with biofilms of 2, 14, and 48 h maturity were treated with mixed and individual bacteriophages at 8 and 9 log10 PFU/mL for 1 h. The results showed reductions greater than 6 log10 CFU/cm2 as a result of exposing SS surfaces with biofilms of 24 h maturity to 9 log10 PFU/mL of bacteriophages; however, the E. coli and bacteriophage strains, phage concentration, and biofilm development stage had significant effects on biofilm reduction (p < 0.05). In conclusion, the isolated bacteriophages showed effectiveness at reducing biofilms of isolated E. coli; however, it is necessary to increase the libraries of phages with lytic activity against the strains isolated from production environments.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Efficacy of Novel Bacteriophages against Escherichia coli Biofilms on Stainless Steel

et al. 2021

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“…59 Simultaneous application of phages as found in several study models show greater efficacy in biofilm destruction than mono phage application. 55,58 In a recent study, phage cocktail composed of four lytic phages, completely inhibited the growth of MDR E. coli and significantly prevented the development of biofilms compared with single phages. The phage mixture caused strong biomass reduction of biofilm and showed the highest biofilm inhibition up to nearly 87%.…”

Section: Application Of Phage Cocktailsmentioning

confidence: 99%

“… 55 Numerous mono phages have been applied to significantly inhibit or decrease to minimum levels of viable bacteria cells in biofilms and in liquid cultures with no considerable toxicity to mammalian cells. Notable among them are biofilms formed by E. faecalis , 56 S. aureus 57 and E. coli , 58 all belonging to the ESKAPEE group. As revealed in these studies, anti-biofilm effects of lytic mono phages are concentration-dependent.…”

Section: Application Of Phages In Bacterial Biofilm Destructionmentioning

confidence: 99%

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Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Amankwah¹,

Abdella²,

Kassa³

2021

NSA

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Biofilms are bacterial communities that live in association with biotic or abiotic surfaces and enclosed in an extracellular polymeric substance. Their formation on both biotic and abiotic surfaces, including human tissue and medical device surfaces, pose a major threat causing chronic infections. In addition, current antibiotics and antiseptic agents have shown limited ability to completely remove biofilms. In this review, the authors provide an overview on the formation of bacterial biofilms and its characteristics, burden and evolution with phages. Moreover, the most recent possible use of phages and phage-derived enzymes to combat bacteria in biofilm structures is elucidated. From the emerging results, it can be concluded that despite successful use of phages and phage-derived products in destroying biofilms, they are mostly not adequate to eradicate all bacterial cells. Nevertheless, a combined therapy with the use of phages and/or phage-derived products with other antimicrobial agents including antibiotics, nanoparticles, and antimicrobial peptides may be effective approaches to remove biofilms from medical device surfaces and to treat their associated infections in humans.

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Nanocomposites: silver nanoparticles and bacteriocins obtained from lactic acid bacteria against multidrug-resistant Escherichia coli and Staphylococcus aureus

Pineda,

Sánchez,

Caycedo

et al. 2024

World J Microbiol Biotechnol

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Efficacy of novel phages for control of multi-drug resistant Escherichia coli O177 on artificially contaminated beef and their potential to disrupt biofilm formation

Cited by 34 publications

References 34 publications

Efficacy of Novel Bacteriophages against Escherichia coli Biofilms on Stainless Steel

Efficacy of Novel Bacteriophages against Escherichia coli Biofilms on Stainless Steel

Bacterial Biofilm Destruction: A Focused Review On The Recent Use of Phage-Based Strategies With Other Antibiofilm Agents

Nanocomposites: silver nanoparticles and bacteriocins obtained from lactic acid bacteria against multidrug-resistant Escherichia coli and Staphylococcus aureus

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