Efficacy of Ovsynch protocol in cyclic and acyclic Egyptian buffaloes in summer

Karen, Aly; Darwish, S.

doi:10.1016/j.anireprosci.2009.12.005

Cited by 26 publications

(14 citation statements)

References 22 publications

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“…This study showed that following GnRH, smaller follicles (8.47 ± 0.53 mm), failed to ovulate as compared to larger DF (10.8 ± 0.27 mm). Our findings are supported by earlier reports in buffaloes (Berber, Madureira, & Baruselli, ; Karen & Darwish, ; Karuppanasamy et al., ). Likewise, administration of eCG 72 hr prior to GnRH1 in Ovsynch Plus protocol accelerated the growth of ovarian follicles and DF diameter increased from 10.15 ± 0.26 mm to 12.23 ± 0.34 mm with better response for GnRH1 (96.6%) in Ovsynch Plus group than Ovsynch group (61.5%).…”

Section: Discussionsupporting

confidence: 93%

“…In this study, three different responses (CL formation, luteinization of follicle or non‐responded) obtained following GnRH administration were similar to the findings in pre‐pubertal buffalo heifers and buffaloes (Karen & Darwish, ; Karuppanasamy et al., ; Sharma, Singh, Khanna, & Singh, ). Administration of GnRH in buffalo results in FSH and LH release within 4 hr after (Singh & Madan, ).…”

Section: Discussionsupporting

confidence: 87%

“…Administration of GnRH in buffalo results in FSH and LH release within 4 hr after (Singh & Madan, ). This LH release following GnRH, either ovulates or luteinizes the DF within 48 hr, in addition to the regression of smaller follicles in anovular buffaloes (Ali & Fahmy, ; Karen & Darwish, ). This study showed that following GnRH, smaller follicles (8.47 ± 0.53 mm), failed to ovulate as compared to larger DF (10.8 ± 0.27 mm).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, ovulation rate in Ovsynch group following GnRH1 was lower than GnRH2 (61.5% vs. 76.9%, p < .01), which was similar to Ali and Fahmy () in anovular buffaloes. However, Karen and Darwish () reported lower ovulation rates to GnRH1 (50%) and GnRH2 (60%) using Ovsynch protocol in anovular buffaloes. This variation can be attributed to DF size at the time of GnRH administration.…”

Section: Discussionmentioning

confidence: 99%

“…Similarly, variable conception rate (0%–37.5%) (Ali & Fahmy, ; De Rensis et al., ; Karen & Darwish, ; Rohilla, Singh, Sharma, & Singh, ) has been reported in anovular buffaloes using Ovsynch protocol. In most studies, lower success rate was obtained during low‐breeding season, and various modified protocols have been tried in quest to overcome these problems (Warriach, Channa, & Ahmad, ).…”

Section: Introductionmentioning

confidence: 92%

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Ovsynch Plus protocol improves ovarian response in anovular Murrah buffaloes in low‐breeding season

Sharma

Phulia

Adnot

et al. 2017

Reprod Domestic Animals

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The objective of the study was to evaluate the efficacy of ovarian response and pregnancy rate in anovular buffaloes following Ovsynch and Ovsynch Plus protocols. Buffaloes (n = 55) were divided into two groups: Ovsynch group (n = 26): GnRH (10 μg, GnRH1) on Day 0, PGF α (25 mg) on Day 7, GnRH (10 μg, GnRH2) on Day 9; Ovsynch Plus group (n = 29): 500 IU equine chorionic gonadotropin (eCG) 72 hr (day -3) prior to Ovsynch protocol, followed by fixed timed artificial insemination (FTAI) 6 and 24 hr after GnRH2 injection in bot groups. Transrectal ultrasonography was performed daily, that is, from day 0 and -3 in Ovsynch and Ovsynch Plus group, respectively for ovarian response and pregnancy diagnosis at day 30 post-insemination. In Ovsynch Plus group, administration of eCG prior to GnRH1 increased (p < .001) the diameter (mm) of dominant follicle (DF) from 10.15 ± 0.26 to 12.23 ± 0.34 within 72 hr of treatment resulting higher ovulatory response to GnRH1. Ovulation after GnRH1 was higher (p < .01) in Ovsynch Plus group (96.6%) than Ovsynch group (61.5%). However, ovulation rate to GnRH2 was similar (p > .05) between groups (Ovsynch group: 76.9% vs. Ovsynch Plus group: 70.0%). Mean DF diameter (mm) that ovulated to both GnRHs was higher (p < .01) than non-ovulated counterparts in both groups (Ovsynch group: 10.80 ± 0.27 vs. 8.47 ± 0.53; Ovsynch Plus group: 11.99 ± 0.24 vs. 9.5 ± 0.63). Pregnancy was established in buffaloes which responded to both GnRHs, irrespective of groups, being higher (p = .52) in Ovsynch Plus group (34.5%) than Ovsynch group (23.1%), though non-significant. In summary, this study showed that eCG inclusion prior to Ovsynch regimen improves ovulatory response in anovular buffaloes during low-breeding season.

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Section: Discussionsupporting

confidence: 93%