In the fight against infectious diseases, with the combined efforts of basic and clinical veterinary scientists, practitioners and domestic animal producers, antimicrobials of natural origin will bring to veterinary medicine the same type of curative revolution as antibiotics did. To realize the main objective of animal husbandry, in diminishing negative economic trends, it is necessary to introduce contemporary management to reproduction. Amongst the numerous preventive measures in use in practice, it is also important to use alternative preparations such as ozone, which has germicidal and fungicidal activities. Ozone is frequently used within the field of veterinary medicine for prophylaxis/treatment of pathological conditions of the genital system in domestic ruminants, such as retained fetal membranes, clinical metritis/endometritis, and improvement of fertility, according to some previous studies. The advantages of ozone in comparison to antibiotics are usage without prescription, low medication costs, lack of bacterial resistance, and the absence of residues in animal products. However, ozone is frequently but still insufficiently used in veterinary practice, particularly in buiatrics. More recently, it has exhibited very promising results in treatment of puerperal disorders in cows, goats and sheep as an alternative to the use of antibiotics, which are restricted and/or being gradually withdrawn in production of food animals in the EU.