Efficacy of Ozone on Mortality, Super Oxide Dismutases, Nitric Oxide Enzymes and Ultrastructural Alterations on Some Stored Product Insect Larvae in Egypt

Ghazawy, N.A.; Zinhoum, Rasha; Ali, Musab A. M.; Afify, A. M.; Hussain, Hassan B.

doi:10.4001/003.029.0017

Cited by 5 publications

(4 citation statements)

References 33 publications

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“…Our findings of cell damage, and the appearance of vacuoles and lysosomal bodies due to cell lysis, were consistent with the effect of ozone exposure on brain tissues, neurosecretory cells, muscles, and fat body in caterpillars and grubs reported by Ghazawy et al [41] A corresponding accumulation of neurosecretory granules inside cells, without release into the axons, also occurred in following farnesol treatment in a study by Awad and Ghazawy [42].…”

Section: Discussionsupporting

confidence: 92%

The Effect of Abamectin on Locusta Migratoria Neurosecretory Cells and Mid Gut, Using Ultrastructure Examination, Oxidative Stress Study, and In-Silico Molecular Docking

Ghazawy,

Afify,

Radwan

et al. 2023

Molecules

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(1) Background: Few studies have been carried out to appraise abamectin toxicity toward Locusta migratoria nymphs. (2) Methods: This study aimed to evaluate the cytotoxic effect of abamectin as an insecticide through examining the changes and damage caused by this drug, in both neurosecretory cells and midgut, using L. migratoria nymphs as a model of the cytotoxic effect. Histopathological change in the brain was examined in both normal and abamectin-treated fifth-instar nymphs. Neurosecretory cells (NSCs) were also examined where there were loosely disintegrated cells or vacuolated cytoplasm. (3) Results: The results showed distinct histological changes in the gastrointestinal tract of L. migratoria nymphs treated with abamectin, with significant cellular damage and disorganization, i.e., characteristic symptoms of cell necrosis, a destroyed epithelium, enlarged cells, and reduced nuclei. The observed biochemical changes included an elevation in all measured oxidative stress parameters compared to untreated controls. The malondialdehyde activities (MDAs) of the treated nymphs had a five- to six-fold increase, with a ten-fold increase in superoxide dismutase (SOD), nine-fold increase in glutathione-S-transferase (GST), and four-fold increase in nitric oxide (NO). (4) Conclusions: To further investigate the theoretical method of action, a molecular docking simulation was performed, examining the possibility that abamectin is an inhibitor of the fatty acid-binding protein Lm-FABP (2FLJ) and that it binds with two successive electrostatic hydrogen bonds.

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Section: Discussionsupporting

confidence: 92%

The Effect of Abamectin on Locusta Migratoria Neurosecretory Cells and Mid Gut, Using Ultrastructure Examination, Oxidative Stress Study, and In-Silico Molecular Docking

Ghazawy,

Afify,

Radwan

et al. 2023

Molecules

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“…In agreement with Husain et al (2015), who tested the effect of exposing date fruits infested with 4th instar larvae of the almond moth, Ephestia cautella (Walker) (Lepidoptera: Pyralidae), to ozone gas at 22 ppm for 24, 48 and 72 hours. 0.0 ± 0.0 e --------0.0 ± 0.0 e ------------- (Ghazawy et al, 2021). In the postembryonic life of insects, neurosecretory cells in the central nervous system regulate several important physiological processes.…”

Section: Large-scale Confirmatory Testsmentioning

confidence: 99%

“…Some insects responded well to fumigation with ozone (Jian et al, 2013). For caterpillars and grubs exposed to ozone, ultrastructural analysis revealed that the muscles, fat body, and neurosecretory cells in the brain were all impacted (Ghazawy et al, 2021). Lipids, proteins, and nucleic acids are oxidized and changed at the molecular and cellular level as a result of ozone action (Iriti and Faoro, 2008).…”

Section: Large-scale Confirmatory Testsmentioning

confidence: 99%

Phytosanitary ozone treatment for guava infested with Bactrocera zonata and mandarin infested with Ceratitis capitata (Diptera: Tephritidae)

Negm,

El-Desouky,

Afify

et al. 2023

SVU-International Journal of Agricultural Sciences

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Both the peach fruit fly, Bactrocera zonata (Saunders), and the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), attack a wide variety of tree fruits worldwide. Exporting fruit hosts of these pests from countries where they are endemic to those where they are absent but may spread requires phytosanitary treatments. This research is to determine if C. capitata and B. zonata could be phytosanitary controlled by ozone treatment schedules. Moreover, elucidates the efficiency of ozone application in controlling microbial growth during the storage of fruits under cooling. Existing results proved that exposing infested fruits (guava and mandarin) with tephritids (C. capitata and B. zonata) to ozone gas for a time reaching 8 hours is sufficient for getting rid of immature stages (eggs, 1 st , 2 nd , and 3 rd larval instars) inside fruits. On the other hand, the results indicated that the ozonation process at 1000 ppm for 6 and 8 hours reduced the total fungal count (TFC) compared to the control samples. Ozone application may be an effective strategy for guava and mandarin fruits to retain post-harvest quality and have a longer period of storage, even though ozone-treated fruits have increased ascorbic acid levels. .

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“…Furthermore, its degradation results in the production of oxygen [14,16]. These traits have led to studies demonstrating the effectiveness of ozone gas in controlling insect pests in stored grains, including C. maculatus, Zabrotes subfasciatus, Rhyzopertha dominica, Ephestia cautella, Plodia interpunctella, Trogoderma granarium, and Tribolium castaneum [17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Ozonation of Cowpea Grains: Alternative for the Control of Callosobruchus maculatus and Maintenance of Grain Quality

Ramos

Silva

et al. 2023

Agriculture

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Nowadays, the modified atmosphere with ozone is one of the main alternatives for controlling insect pests in stored grains, as it allows grain quality to be maintained while causing no environmental damage. In light of this, the purpose of this study was to assess the toxicity of ozone to C. maculatus in cowpea grains as well as how it affects the physiological and physical properties of the grains. The toxicity was determined by estimating lethal doses (mg/g) at the top and bottom of the fumigation chamber for 50% and 95% of adult insects (LD50 and LD95). Cowpea grain samples were removed after being exposed to ozone, and the effect of ozonation on the physical and physiological quality of the grains was assessed. The electrical conductivity, water content, and germination were all examined in this manner. The lethal doses LD50 were 65.97 and 45.52 mg/g when the insects were distributed at the top and bottom of the fumigation chamber, respectively. As a result, ozone toxicity was higher at the bottom than at the top. The increase in ozone doses resulted in a decrease in water content. The germination rate exceeded 90% in both locations where ozone was applied. The electrical conductivity remained constant, ranging between 152.01 and 239.59 S cm−1 g −1. The findings indicate that ozone is toxic to C. maculatus but has no effect on grain quality. Ozone may therefore be used to manage C. maculatus in cowpea that has been stored.

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Efficacy of Ozone on Mortality, Super Oxide Dismutases, Nitric Oxide Enzymes and Ultrastructural Alterations on Some Stored Product Insect Larvae in Egypt

Cited by 5 publications

References 33 publications

The Effect of Abamectin on Locusta Migratoria Neurosecretory Cells and Mid Gut, Using Ultrastructure Examination, Oxidative Stress Study, and In-Silico Molecular Docking

The Effect of Abamectin on Locusta Migratoria Neurosecretory Cells and Mid Gut, Using Ultrastructure Examination, Oxidative Stress Study, and In-Silico Molecular Docking

Phytosanitary ozone treatment for guava infested with Bactrocera zonata and mandarin infested with Ceratitis capitata (Diptera: Tephritidae)

Ozonation of Cowpea Grains: Alternative for the Control of Callosobruchus maculatus and Maintenance of Grain Quality

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