Efficacy of phage therapy in poultry: a systematic review and meta-analysis

Mosimann, Sabrina; Desiree, Karina; Ebner, Paul

doi:10.1016/j.psj.2021.101472

Cited by 14 publications

(13 citation statements)

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“…On the other hand, antibiotics are still important drugs used to combat infectious diseases in veterinary medicine, including poultry breeding ( Deblais et al., 2020 ). Irrespective of many reports published previously on the use of bacteriophages to combat infections caused by S. enterica in poultry (summarized recently by Mosimann et al., 2021 ), those studies tested the effects of applications of phages to birds, without comparing them to those caused by antibiotics. Therefore, in this work, we compared biological aspects of phage therapy and the use of antibiotics in a large study on chickens infected experimentally with S. Typhimurium ( Figure 1 ).…”

Section: Discussionmentioning

confidence: 99%

“…The term ‘phage therapy and chicken’ gave in the PubMed database ( ) over 100 records (as on May 7, 2022). A substantial part of these publications concerned the treatment of S. enterica infections, and the results in this field published to date have been summarized recently ( Mosimann et al., 2021 ). The general conclusions made on the basis of that review of already performed studies were that the use of the phage therapy may lead to significant reduction of the number of living pathogenic bacteria in chickens and that profitable effects may be more pronounced in the short-term period and in older birds.…”

Section: Introductionmentioning

confidence: 99%

“…Despite quite a large body of data on the effects on the administration of bacteriophages in poultry ( Mosimann et al., 2021 ), the vast majority of the reported studies on the phage therapy against Salmonella in chickens concerned solely effects of bacteriophages under various conditions. However, antibiotic therapy is still an important option in aviculture when bacterial infections occur.…”

Section: Introductionmentioning

confidence: 99%

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Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens

Kosznik-Kwaśnicka

Podlacha

Grabowski

et al. 2022

Front. Cell. Infect. Microbiol.

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Phage therapy is a promising alternative treatment of bacterial infections in human and animals. Nevertheless, despite the appearance of many bacterial strains resistant to antibiotics, these drugs still remain important therapeutics used in human and veterinary medicine. Although experimental phage therapy of infections caused by Salmonella enterica was described previously by many groups, those studies focused solely on effects caused by bacteriophages. Here, we compared the use of phage therapy (employing a cocktail composed of two previously isolated and characterized bacteriophages, vB_SenM-2 and vB_Sen-TO17) and antibiotics (enrofloxacin and colistin) in chickens infected experimentally with S. enterica serovar Typhimurium. We found that the efficacies of both types of therapies (i.e. the use of antibiotics and phage cocktail) were high and very similar to one another when the treatment was applied shortly (one day) after the infection. Under these conditions, S. Typhimurium was quickly eliminated from the gastrointestinal tract (GIT), to the amount not detectable by the used methods. However, later treatment (2 or 4 days after detection of S. Typhimurium in chicken feces) with the phage cocktail was significantly less effective. Bacteriophages remained in the GIT for up to 2-3 weeks, and then were absent in feces and cloaca swabs. Interestingly, both phages could be found in various organs of chickens though with a relatively low abundance. No development of resistance of S. Typhimurium to phages or antibiotics was detected during the experiment. Importantly, although antibiotics significantly changed the GIT microbiome of chickens in a long-term manner, analogous changes caused by phages were transient, and the microbiome normalized a few weeks after the treatment. In conclusion, phage therapy against S. Typhimurium infection in chickens appeared as effective as antibiotic therapy (with either enrofloxacin or colistin), and less invasive than the use the antibiotics as fewer changes in the microbiome were observed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens

Kosznik-Kwaśnicka

Podlacha

Grabowski

et al. 2022

Front. Cell. Infect. Microbiol.

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“…The low ability of phages to reduce pathogens in internal organs may be the result of the method of bacteriophage administration to chickens. Meta-analytics suggested that the administration route may significantly impact phage treatment efficacy (Mosimann et al, 2021). Borie et al (2008) reported that although a bacteriophage cocktail containing phages BP1, BP2, and BP3 significantly reduced the number of S. Enteritidis in birds treated with phages administered via coarse spray or drinking water, the latter method was less effective in the elimination of this pathogen from internal organs, partially due to phage inactivation attributed to stomach acidity.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Bacteriophage Cocktail Can Effectively Control Salmonella Biofilm in Poultry Housing

Korzeniowski

Śliwka²,

Kuczkowski³

et al. 2022

Front. Microbiol.

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Salmonella enterica serovar Enteritidis (S. Enteritidis) is the major contaminant of poultry products, and its ability to form biofilms on produced food and poultry farm processing surfaces contributes to Salmonella transmission to humans. Bacteriophages have come under increasing interest for anti-Salmonella biofilm control. In this study, we used the three previously sequenced and described phages UPWr_S1, UPWr_S3, and UPWr_S4 and a phage cocktail, UPWr_S134, containing these three phages to degrade biofilms formed by two S. Enteritidis strains, 327 lux and ATCC 13076, in vitro. It was found that treatment with bacteriophages significantly reduced biofilm on a 96-well microplate (32–69%) and a stainless steel surface (52–98%) formed by S. Enteritidis 327 lux. The reduction of biofilm formed by S. Enteritidis ATCC 13076 in the 96-well microplate and on a stainless steel surface for bacteriophage treatment was in the range of 73–87% and 60–97%, respectively. Under laboratory conditions, an experimental model utilizing poultry drinkers artificially contaminated with S. Enteritidis 327 lux and treated with UPWr_S134 phage cocktail was applied. In in vitro trials, the phage cocktail significantly decreased the number of Salmonella on the surface of poultry drinkers. Moreover, the phage cocktail completely eradicated Salmonella from the abundant bacterial load on poultry drinkers in an experimentally infected chickens. Therefore, the UPWr_S134 phage cocktail is a promising candidate for Salmonella biocontrol at the farm level.

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Prophylactic phage administration reduces Salmonella Enteritidis infection in newly hatched chicks

Agapé,

Menanteau,

Kempf

et al. 2024

MicrobiologyOpen

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Salmonellosis outbreaks are global issues primarily associated with the consumption of poultry products, which may be infected with Salmonella. The use of lytic bacteriophages could be a safe and effective approach to reduce Salmonella prevalence in poultry and subsequently the incidence in humans. This study examined the value of prophylactic phage treatment on Salmonella levels in chickens and the effect of such treatment on their overall gut microbiome. We also investigated phage persistence in vivo and resistance emergence against the six‐phage cocktail used. The preventive potential of phages was evaluated on 200 chicks by administering phages via drinking water for 6 days after hatching, followed by the Salmonella Enteritidis challenge on Day 7. The results showed that up to 4 days postinfection, phages had a preventive effect by significantly reducing Salmonella colonization in ceca by three logs. Furthermore, the phage cocktail did not induce dysbiosis, although variations in microbiota in terms of microbial composition were observed between conditions, with the Enterobacteriaceae family being impacted. However, the phage cocktail did not induce a long‐term effect, with Salmonella levels rebounding 8 days after phage treatment was stopped. Overall, our data show that phage prophylaxis can reduce Salmonella colonization and explore ways of improving the effectiveness of phages in limiting infections throughout poultry production.

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Efficacy of phage therapy in poultry: a systematic review and meta-analysis

Cited by 14 publications

References 40 publications

Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens

Biological aspects of phage therapy versus antibiotics against Salmonella enterica serovar Typhimurium infection of chickens

Bacteriophage Cocktail Can Effectively Control Salmonella Biofilm in Poultry Housing

Prophylactic phage administration reduces Salmonella Enteritidis infection in newly hatched chicks

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