Efficacy of Platelet-rich Plasma as a Scaffold in Regenerative Endodontic Treatment

Bezgin, Tuğba; Yılmaz, Ayça; Çelik, Burcu; Kolsuz, Mehmet Eray; Sönmez, Hayriye

doi:10.1016/j.joen.2014.10.004

Cited by 150 publications

(230 citation statements)

References 34 publications

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“…Although the experimental scaffolds containing MINO or DOX did not prevent dentin discoloration, this strategy carries clinical potential for the treatment of necrotic teeth. The replacement of MINO by other antibiotics, such as Augmentin (27), is under investigation as a potential discoloration-free strategy. The method used in this study to measure color change was based on the CIE L * a * b * color system, which is a device-independent system.…”

Section: Discussionmentioning

confidence: 99%

Effects of Novel 3-dimensional Antibiotic-containing Electrospun Scaffolds on Dentin Discoloration

Porter

Münchow

Albuquerque

et al. 2016

Journal of Endodontics

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Introduction Although intracanal application of the triple antibiotic paste (TAP) may offer advantages (e.g., disinfection), this practice has been associated with significant drawbacks, including tooth discoloration. In this study, the color change of dentin was monitored during treatment with distinct TAP pastes and novel tubular-shaped three-dimensional (3D) electrospun scaffolds containing minocycline-MINO or doxycycline-DOX. Methods Two TAP pastes (TAPMINO–MINO, metronidazole/MET, and ciprofloxacin/CIP; and TAPDOX–DOX, MET, and CIP), four scaffold-based groups containing MINO or DOX, at distinct concentrations; one antibiotic-free scaffold (Scaffold); and one untreated group (Control) were investigated. Human canines were sectioned at the cemento-enamel junction (CEJ) and tubular-shaped scaffolds or paste were placed into the root canals and sealed. Color measurements (CIEL*a*b* parameters) were performed at baseline and after 1, 3, 7, 14, 21, and 28 days. Color changes were expressed as ΔE* values. In addition, scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS) were also performed on the specimens after treatment. Data were analyzed using Repeated-measures ANOVA (alpha=0.05). Results All antibiotic-containing groups led to greater discoloration than the antibiotic-free groups. A severe discoloration occurred after 1 day. At the end of the experiment, antibiotic-treated samples exhibited crusts/agglomerates over the dentin surface, which totally or partially obliterated the dentinal tubules. The presence of MINO resulted in greater color change than DOX. Conclusion Scaffolds containing MINO or DOX produced similar color change to dentin when compared to their respective TAP systems, although DOX-related discoloration was less pronounced.

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Section: Discussionmentioning

confidence: 99%

Effects of Novel 3-dimensional Antibiotic-containing Electrospun Scaffolds on Dentin Discoloration

Porter

Münchow

Albuquerque

et al. 2016

Journal of Endodontics

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“…3N, inset). Studies removing or replacing MINO by Augmentin or Cefaclor as intracanal medications have started as potential alternatives to prevent and/or minimize tooth discoloration (32, 33). Indeed, from an antimicrobial viewpoint, this novel TAP-mimic scaffold demonstrated a significant reduction in viable biofilm, even inside dentinal tubules (Fig.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Effects of Novel Triple Antibiotic Paste–Mimic Scaffolds on Actinomyces naeslundii Biofilm

Albuquerque

Ryan

Münchow

et al. 2015

Journal of Endodontics

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Introduction Actinomyces naeslundii has been recovered from traumatized permanent teeth diagnosed with necrotic pulps. In this work, a triple antibiotic paste (TAP)–mimic scaffold is proposed as a drug-delivery strategy to eliminate A. naeslundii dentin biofilm. Methods Metronidazole, ciprofloxacin, and minocycline were added to a polydioxanone (PDS) polymer solution and spun into fibrous scaffolds. Fiber morphology, mechanical properties, and drug release were investigated by using scanning electron microscopy, microtensile testing, and high-performance liquid chromatography, respectively. Human dentin specimens (4 × 4 × 1 mm3, n = 4/group) were inoculated with A. naeslundii (ATCC 43146) for 7 days for biofilm formation. The infected dentin specimens were exposed to TAP-mimic scaffolds, TAP solution (positive control), and pure PDS (drug-free scaffold). Dentin infected (7-day biofilm) specimens were used for comparison (negative control). Confocal laser scanning microscopy was done to determine bacterial viability. Results Scaffolds displayed a submicron mean fiber diameter (PDS = 689 ± 312 nm and TAP-mimic = 718 ± 125 nm). Overall, TAP-mimic scaffolds showed significantly (P ≤ .040) lower mechanical properties than PDS. Within the first 24 hours, a burst release for all drugs was seen. A sustained maintenance of metronidazole and ciprofloxacin was observed over 4 weeks, but not for minocycline. Confocal laser scanning microscopy demonstrated complete elimination of all viable bacteria exposed to the TAP solution. Meanwhile, TAP-mimic scaffolds led to a significant (P < .05) reduction in the percentage of viable bacteria compared with the negative control and PDS. Conclusions Our findings suggest that TAP-mimic scaffolds hold significant potential in the eradication/elimination of bacterial biofilm, a critical step in regenerative endodontics.

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“…Frente a esta situación, la utilización de plasma rico en plaquetas (PRP) ha sido estudiada, mostrando un gran potencial para utilización como scaffold para la revascularización (44,45). El PRP tiene un volumen de plasma autólogo con altas concentraciones de plaquetas y de factores de crecimiento que promueven la proliferación de células madre (46,47). Los factores de crecimiento liberados por el PRP juegan un papel importante en los procesos de mitogénesis, quimiotaxis, diferenciación y metabolismo celular (44).…”

Section: Revascularización Pulparunclassified

“…La necesidad de colecta de sangre en pacientes jóvenes, equipo especial y alto costo del tratamiento podrían ser considerados como desventajas de esta terapia. Sin embargo, en base a estudios clínicos y radiográ-fi cos, estos materiales son una importante opción en casos en los que la inducción del sangramiento no tuvo éxito (47,50,51).…”

Section: Revascularización Pulparunclassified

Alternativas clínicas para el tratamiento de dientes traumatizados con rizogénesis incompleta: una visión actualizada

Pereira

Herrera

Neto

et al. 2017

Rev Estomatol Herediana.

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RESUMENLa necrosis pulpar en dientes con ápice abierto representa un desafi o al tratamiento endodóntico. Esto debido a que la anatomía radicular se encuentra parcialmente formada, con ápices abiertos, paredes radiculares fi nas y frágiles, así como la longitud radicular reducida. En estas situaciones, cuatro opciones de tratamiento están disponibles: 1) Apexifi cación con renovación periódica de medicación intraconducto (MIC), hasta que sea posible observarradiográfi camente el cierre apical; 2) Apexifi cación sin renovación de MIC; 3) Confección de una barrera apical utilizando agregado de trióxido mineral (MTA) o Biodentine; 4) Revascularización pulpar. El endodoncista debe estar atento a la evolución tanto de las técnicas de tratamiento como de los materiales dentales, conociendo sus indicaciones y limitaciones. Este artículo llevó a cabo una revisión de la literatura sobre las diferentes formas de tratamiento en casos de dientes traumatizados con rizogénesis incompleta y necesidad de tratamiento endodóntico por necrosis pulpar, indicando las ventajas, desventajas y limitaciones de cada una.PALABRAS CLAVE: Apexifi cación, hidróxido de calcio, materiales biocompatibles.

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Efficacy of Platelet-rich Plasma as a Scaffold in Regenerative Endodontic Treatment

Cited by 150 publications

References 34 publications

Effects of Novel 3-dimensional Antibiotic-containing Electrospun Scaffolds on Dentin Discoloration

Effects of Novel 3-dimensional Antibiotic-containing Electrospun Scaffolds on Dentin Discoloration

Antimicrobial Effects of Novel Triple Antibiotic Paste–Mimic Scaffolds on Actinomyces naeslundii Biofilm

Alternativas clínicas para el tratamiento de dientes traumatizados con rizogénesis incompleta: una visión actualizada

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