Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens

Shim, Doo-Hee; Ko, Hyun–Jeong; Gerdts, Volker; Potter, Andrew; Mutwiri, George; Babiuk, Lorne A.; Kweon, Mi‐Na

doi:10.1016/j.vaccine.2009.12.069

Cited by 34 publications

(20 citation statements)

References 35 publications

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“…HIV, malaria or tuberculosis (TB)) that require T cell responses for protective immunity [2,39]. In line with other studies investigating polyphosphazenes including PCPP in mice [40], we demonstrated that PCPP e alone and in formulation with Gag antigen e can promote mixed Th1/Th2 responses in human APCs, showing promise for potentially driving both T cell mediated and humoral immunity in both adults and infants.…”

Section: Discussionsupporting

confidence: 83%

The effect of stable macromolecular complexes of ionic polyphosphazene on HIV Gag antigen and on activation of human dendritic cells and presentation to T-cells

et al. 2014

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Section: Discussionsupporting

confidence: 83%

The effect of stable macromolecular complexes of ionic polyphosphazene on HIV Gag antigen and on activation of human dendritic cells and presentation to T-cells

et al. 2014

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“…Serum and vaginal wash samples were obtained, and Ag-specific Ab titers were determined by ELISA as described elsewhere (26). The plates (BD Falcon, Mississauga, ON, Canada) were coated with 10 mg/ml OVA or 2 mg/ml CTB (List Biological Laboratories) in PBS and incubated overnight at 4˚C.…”

Section: Elisamentioning

confidence: 99%

Mucosa-Associated Epithelial Chemokine/CCL28 Expression in the Uterus Attracts CCR10+ IgA Plasma Cells following Mucosal Vaccination via Estrogen Control

Cha

Kim

et al. 2011

The Journal of Immunology

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Previous studies demonstrated cross talk between mucosal and reproductive organs during secretory IgA Ab induction. In this study, we aimed to clarify the underlying mechanisms of this cross talk. We found significantly higher titers of Ag-specific secretory IgA Ab in the vaginal wash after mucosal vaccination by both the intranasal (i.n.) and the intravaginal routes but not by the s.c. route. Interestingly, Ag-specific IgA Ab-secreting cells (ASCs) were found mainly in the uterus but not in the cervix and vaginal canal after i.n. vaccination. The fact that most Ag-specific IgA ASCs isolated from the uteri of vaccinated mice migrated toward mucosa-associated epithelial chemokine (MEC)/CCL28 suggests dominant expression of CCR10 on the IgA ASCs. Further, IgA ASCs in the uteri of vaccinated mice were reduced drastically in mice treated with neutralizing anti-MEC/CCL28 Ab. Most intriguingly, the female sex hormone estrogen directly regulated MEC/CCL28 expression and was augmented by i.n. vaccination with cholera toxin or stimulators for innate immunity. Further, blockage of estrogen function in the uterus by oral administration of the estrogen antagonist raloxifene significantly inhibited migration of Ag-specific IgA ASCs after i.n. vaccination with OVA plus cholera toxin. Taken together, these data strongly suggest that CCR10+ IgA ASCs induced by mucosal vaccination via the i.n. route migrate into the uterus in a MEC/CCL28-dependent manner and that estrogen might have a crucial role in the protection against genital infection by regulating MEC/CCL28 expression in the uterus.

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“…In mice, a mixture of FHA and PT in combination with chitosan administered nasally induced systemic and mucosal antibody responses to these two antigens, which were much lower in the absence of chitosan (Jabbal‐Gill et al ., ). Similar results were found when pertussis vaccines were administered intranasally together with polyphosphazene as adjuvant (Shim et al ., ). This formulation elicited a mixed Th1/Th2 response and strong protection against respiratory infection with B. pertussis .…”

Section: Novel Pertussis Vaccinesmentioning

confidence: 97%

New pertussis vaccination approaches: en route to protect newborns?

Locht

Mielcarek

2012

FEMS Immunol Med Microbiol

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Pertussis or whooping cough is a life-threatening childhood disease, particularly severe during the first months of life, although adolescent and adult pertussis is increasingly more noted. General vaccination has tremendously reduced its incidence but has failed to bring it completely under control. In fact, it remains one of the most poorly controlled vaccine-preventable diseases in the world. New vaccination strategies are thus being explored. These include vaccination of pregnant mothers to transmit protective antibodies to the offspring, a cocooning strategy to prevent the transmission of the disease from family members to the newborn and neonatal vaccination. All have their inherent limitations, and improved vaccines are urgently needed. Two types of pertussis vaccines are currently available, whole-cell, first-generation and second-generation, acellular vaccines, with an improved safety profile. Attempts have been made to discover additional protective antigens to the 1-5 currently included in the acellular vaccines or to include new adjuvants. Recently, a live attenuated nasal Bordetella pertussis vaccine has been developed and undergone first-in-man clinical trials. However, as promising as it may be, in order to protect infants against severe disease, a single approach may not be sufficient, and multiple strategies applied in a concerted fashion may ultimately be required.

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Efficacy of poly[di(sodium carboxylatophenoxy)phosphazene] (PCPP) as mucosal adjuvant to induce protective immunity against respiratory pathogens

Cited by 34 publications

References 35 publications

The effect of stable macromolecular complexes of ionic polyphosphazene on HIV Gag antigen and on activation of human dendritic cells and presentation to T-cells

The effect of stable macromolecular complexes of ionic polyphosphazene on HIV Gag antigen and on activation of human dendritic cells and presentation to T-cells

Mucosa-Associated Epithelial Chemokine/CCL28 Expression in the Uterus Attracts CCR10+ IgA Plasma Cells following Mucosal Vaccination via Estrogen Control

New pertussis vaccination approaches: en route to protect newborns?

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