Efficacy of Polyvalent Bacteriophage P-27/HP to Control Multidrug Resistant Staphylococcus aureus Associated with Human Infections

Abstract

Emergence of multidrug resistant strains has created serious problem for safe eradication of Staphylococcus aureus infections. Therefore, there is an urgent need to develop novel antibacterial agents to control this pathogen. Bacteriophages kill bacteria irrespective of their antibiotic sensitivity and thus they can be used as potent prophylactic/therapeutic agent to treat such infections. Here, we report isolation of broad host range anti-staphylococcal lytic bacteriophage P-27/HP from sewage water. This phage was able to inhibit 17 of 28 (60%) human disease associated S. aureus isolates. In vitro studies revealed its strong lytic efficacy to diminish S. aureus 27/HP population (c.f.u.) by more than 5.0 logs (P < 0.0001) (equivalent to 99.99%) in 3 h at 0.01 MOI. In vivo lytic efficacy analysis showed that a single subcutaneous injection of phage P-27/HP (10(7) p.f.u.) was sufficient to protect S. aureus 27/HP infected (5 × 10(8) c.f.u.) mice from bacteremia and subsequent death. A considerable decline of more than 6 logs (99.9%) in splenic S. aureus 27/HP c.f.u. count was noted at the 3 days of phage treatment. In conclusion, our results suggest that phage P-27/HP is polyvalent in nature and has high-lytic potential towards S. aureus, thus, a therapy employing this phage would be efficacious to control S. aureus infections.

Combined Use of Bacteriophage K and a Novel Bacteriophage To Reduce Staphylococcus aureus Biofilm Formation

“…Several studies have shown the potential of using phages to treat S. aureus infections (21)(22)(23), and it has been demonstrated that phages also have the ability to disrupt bacterial biofilms (24). Phages are increasingly recognized as serious candidates in the fight against antibiotic-resistant bacteria in human therapeutics and as prophylaxis (25).…”

Complete Genome Sequence of Wide-Host-Range Staphylococcus aureus Phage JD007

“…Attempts to use bacteriophages to treat infectious diseases have been made since they were discovered (5), and they are still being used in some former Soviet Union countries like Georgia and Poland. Some clinical tests and animal models have proved that S. aureus phage can effectively treat infectious diseases caused by S. aureus, particularly some multidrug-resistant strains (2,3,7,9,11,12). S. aureus phage JD007 was isolated from chicken feces in Shanghai, China.…”

Characterization of Methicillin-Resistant Staphylococcus aureus (MRSA) Phages From Sewage at a Tertiary Pediatric Hospital

“…Phages specifically recognize their hosts, and any region has specific phages; previous publications have demonstrated that any region has specific phages against MRSA and phages have specific hosts (13)(14)(15) reus belong to the order Caudovirales and consist of doublestranded DNA located in the icosahedral capsid. According to the tail morphology, they can be classified into three families, namely Podoviridae, Siphoviridae, and Myoviridae (16).…”