Efficacy of red propolis hydro-alcoholic extract in controlling Streptococcus mutans biofilm build-up and dental enamel demineralization

Martins, Mariana Leonel; Leite, Karla Lorene de França; Pacheco-Filho, Edivaldo Ferreira; Pereira, Adriana Farah de Miranda; Romanos, Maria Teresa Villela; Maia, Lucianne Cople; Fonseca‐Gonçalves, Andréa; Padilha, Wílton Wilney Nascimento; Cavalcanti, Yuri Wanderley

doi:10.1016/j.archoralbio.2018.05.017

Cited by 53 publications

(56 citation statements)

References 55 publications

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“…Although chlorhexidine have been extensively used for biofilm control, its toxic potential to the mucosa contra-indicates its use for prolonged periods [13]. Brazilian red propolis hydroalcoholic extract at 3% has demonstrated a cytotoxic activity around 43% against fibroblasts monolayers (L-929) [16]. It is worth emphasizing that the implementation of an antimicrobial therapy must modulate the cell proliferation, so that when it debilitates the infection, there must not be great damage to the subjacent tissue.…”

Section: Discussionmentioning

confidence: 99%

“…The hydroalcoholic extract of Brazilian red propolis (Laboratory Edimel, Paraíba, Brazil) was obtained at initial concentration of 30% (w/v) and diluted in sterile saline to yield concentration of 3% (w/v), as determined previously [16]. The sterile saline was used as growth control of biofilm.…”

Section: Expositions To Tested Substancesmentioning

confidence: 99%

“…In this context, the Brazilian red propolis has demonstrated an inhibitory effect against mono-species biofilm of C. albicans in a similar manner to that of chlorhexidine [16][17][18]. Propolis is a natural resin produced by bees [19], with antitumor, anti-oxidative and antimicrobial activities [20].…”

Section: Introductionmentioning

confidence: 99%

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“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

Martorano-Fernandes

Cavalcanti

Almeida

2020

BMC Complement Med Ther

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Background: Peri-implant inflammation resulting from the presence of Candida biofilms may compromise the longevity of implant-supported dentures. This study evaluated the inhibitory effect of Brazilian red propolis on mono-species biofilms of C. albicans (ATCC 90028) and co-culture biofilms of C. albicans (ATCC 90028) and C. glabrata (ATCC 2001), developed on titanium surfaces. Methods: Titanium specimens were pre-conditioned with artificial saliva and submitted to biofilm formation (1 × 10 6 CFU/mL). After 24 h (under microaerophilic conditions at 37°C) biofilms were submitted to treatment for 10 min, according to the groups: sterile saline solution (growth control), 0.12% chlorhexidine and 3% red propolis extract. Treatments were performed every 24 h for 3 days and analyses were conducted 96 h after initial adhesion. After that, the metabolic activity (MTT assay) (n = 12/group), cell viability (CFU counts) (n = 12/group) and surface roughness (optical profilometry) (n = 6/group) were evaluated. Data from viability and metabolic activity assays were evaluated by ANOVA and Tukey tests. Surface roughness analysis was determined by Kruskal Wallis e Mann Whitney tests. Results: Regarding the mono-species biofilm, the cell viability and the metabolic activity showed that both chlorhexidine and red propolis had inhibitory effects and reduced the metabolism of biofilms, differing statistically from the growth control (p < 0.05). With regards the co-culture biofilms, chlorhexidine had the highest inhibitory effect (p < 0.05). The metabolic activity was reduced by the exposure to chlorhexidine and to red propolis, different from the growth control group (p < 0.05). The surface roughness (Sa parameter) within the mono-species and the co-culture biofilms statistically differed among groups (p < 0.05). Conclusions: Brazilian red propolis demonstrated potential antifungal activity against Candida biofilms, suggesting it is a feasible alternative for the treatment of peri-implantitis.

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Section: Discussionmentioning

confidence: 99%

Section: Expositions To Tested Substancesmentioning

confidence: 99%

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“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

Martorano-Fernandes

Cavalcanti

Almeida

2020

BMC Complement Med Ther

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“…7 In efforts to prevent caries, several natural products have been analyzed against cariogenic bacteria. [8][9][10] Nudibranchs present an extensive chemical diversity with terpenes been the major metabolites isolated, however there are many nudibranchs yet to be studied. 11 The Brazilian coast has been poorly explored, with only 200 species reported.…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition and Anticariogenic Activity of Tambja stegosauriformis Nudibranch

Braga

Iório²,

Póvoa³

et al. 2019

Rev. Virtual Quim.

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1457 Artigo Chemical Composition and Anticariogenic Activity of Tambja stegosauriformis Nudibranch Braga, R. R.;* Iorio, N. L. P. P; Póvoa, H. C. C.; Chianca, G. C.; Kachlicki, P.; Ożarowski, M.; Silva, V. O.; Félix, H. P.; Lopes, I. C.; Chaves, D. S. A.Resumo: Extrato do nudibrânquio da espécie Tambja stegosauriformis foi obtido por imersão em metanol, concentrados em um rotaevaporador e secos por liofilização. A identificação dos metabólitos secundários presentes nos extratos foi realizada por cromatografia líquida de alta eficiência com detector DAD, acoplada à espectrometria de massa (HPLC-DAD-MSn). Foram identificados oito alcaloides pirrólicos no extrato de T. stegosauriformis. Todos os alcaloides derivam da estrutura base com dois anéis pirrólicos interligados e presença de bromo ou não, as quais foram reportadas previamente na literatura. A atividade antibacteriana do extrato foi testada em amostras padrão de Strepcococcus mutans ATCC ("American Type Culture Collection") 25175 e Lactobacillus casei ATCC 393, determinando a Concentração Inibitória Mínima (CIM) e Concentração Bactericida Mínima (CBM) do extrato frente a essas bactérias. A atividade citotóxica das concentrações do extrato de T. stegosauriformis com atividade antibacteriana foi verificada em Artemia salina. O extrato de T. stegosauriformis apresentou atividade bacteriostática frente às espécies S. mutans (CIM = 1,175mg / mL) e L. casei (CIM = 0,5875 mg / mL). Os resultados indicam um potencial efeito anticariogênico de substâncias presentes no extrato do nudibrânquio T. stegosauriformis Palavras-chave: Nudibrânquios; produtos naturais marinhos; alcaloides pirrólicos; atividade antimicrobiana AbstractExtract from Tambja stegosauriformis nudibranch were obtained by immersion in methanol, concentrated in a rotary evaporator and dried by lyophilization. The identification of the secondary metabolites present in the extracts was performed by high performance liquid chromatography with DAD detector, coupled to mass spectrometry (HPLC-DAD-MSn). Eight pyrrole alkaloids were identified in the T. stegosauriformis extract. All alkaloids identified and reported previously in the literature are derived from the base structure of two interconnected pyrrole rings, some compounds are substituted with bromine. The antibacterial activity of the extracts was tested on Streptococcus mutans ATCC (American Type Culture Collection) 25175 and Lactobacillus casei ATCC 393 samples, determining the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of the extract against these bacteria. The cytotoxic activity against Artemia salina was detected in the T. stegosauriformis extract with antibacterial activity. The T. stegosauriformis extract presented bacteriostatic activity against S. mutans (MIC = 1.175mg / mL) and L. casei (MIC = 0.5875 mg / mL). The results indicate a potential anticariogenic effect of substances present in the T. stegosauriformis extract.

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“…It was previously observed that CHX is able to reduce lactic acid production after a short period of time as well, but it is not effective after long periods in vivo (GERARDU et al, 2007;GEORGIOS;VASSILIKI;SOTIRIOS, 2015). Martins et al (2018) found reduction of soluble and insoluble EPS production after daily treatment with CHX using monospecies biofilm, but nothing is known about the effect of a unique application under microcosm biofilm. It is likely that CHX, applied only once before microcosm biofilm formation, has no enough substantivity to interfere inside the cells, but only on their membrane affecting EPS production only.…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial and anti-caries effects of 4% titanium tetrafluoride varnish under a microcosm biofilm model on dentin

Santos¹

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Antimicrobial and anti-caries effects of 4% titanium tetrafluoride varnish under a microcosm biofilm model on dentin Efeitos antimicrobiano e anti-cárie do verniz tetrafluoreto de titânio a 4% sob um modelo de biofilme microcosmo em dentina BAURU 2018 DAIANA MORELI SOARES DOS SANTOS Antimicrobial and anti-caries effects of 4% titanium tetrafluoride varnish under a microcosm biofilm model on dentinEfeitos antimicrobiano e anti-cárie do verniz tetrafluoreto de titânio a 4% sob um modelo de biofilme microcosmo em dentina

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Efficacy of red propolis hydro-alcoholic extract in controlling Streptococcus mutans biofilm build-up and dental enamel demineralization

Cited by 53 publications

References 55 publications

“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

“Inhibitory effect of Brazilian red propolis on Candida biofilms developed on titanium surfaces”

Chemical Composition and Anticariogenic Activity of Tambja stegosauriformis Nudibranch

Antimicrobial and anti-caries effects of 4% titanium tetrafluoride varnish under a microcosm biofilm model on dentin

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