B otanical pesticides are ideal for organic farming since they are less dangerous to humans, disintegrate quickly, and have fewer residual effects with the least adverse environmental impact than chemical insecticides (Akbar et al., 2021). Pyrethrum, rotenone, hellebore, nicotine, veratrum alkaloids, Abstract | The current study was conducted to evaluate the toxicity and repellency of leaf aqueous extracts i.e., Melia azedarach (Bakain), Nicotiana tabacum L. (Tobacco), Eucalyptus globulus Labill (Eucalyptus), Moringa oleifera L. (Moringa) and Mentha piperita L. (Peppermint) against two major sucking pests i.e. aphid (Sitobion avenae F.) and two-spotted spider mites (Tetranychus urticae Koch) and their natural enemies such as ladybird beetle (Coccinella septempunctata Linnaeus) and predatory mite (Amblyseius andersoni Chant). The leaf dip method was used to conduct bioassay and this experiment was performed under Complete Randomized Design (CRD) with three replications. The results showed that N. tabacum extract was more toxic to aphids, two-spotted spider mites, ladybird beetles and predatory mites and caused 80.82, 81.49, 39.77 and 51.55% mortalities, respectively, after 96 hours. M. azedarach extract showed the second highest mortalities i.e., 73.85% in aphids, 70.54% in two-spotted spider mites, 31.05% in ladybird beetles and 46.49% of predatory mites. Mortality trend observed in other aqueous extracts was eucalyptus > moringa > peppermint. The repellent effect of these extracts was also evaluated and observed after 12 and 24 hours. N. tabacum extract caused 80.55 and 86.88% repellencies against aphid and two-spotted spider mites, while 68.55 and 74.34% against ladybird beetle and predatory mites, followed by bakain > peppermint > moringa > eucalyptus. The results revealed that all these leaf aqueous extracts can be used effectively against aphids and two-spotted spider mites, but we have to be careful with their slightly toxic effects on predators.