Efficacy of spheroplastic and cell-wall competent vaccines for Mycobacterium avium subsp. paratuberculosis in experimentally-challenged baby goats

Hines, Murray E.; Stiver, Shane; Giri, Dipak K.; Whittington, Lisa; Watson, Cindy; Johnson, Joyce; Musgrove, Julie; Pence, Mel E.; Hurley, David J.; Baldwin, Charles A.; Gardner, Ian A.; Aly, Sharif S.

doi:10.1016/j.vetmic.2006.10.030

Cited by 39 publications

(33 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The tube should be reweighed to determine the wet pellet weight. In general, the number of MAP would range from 5 Â 10 6 (Juste et al, 1994) to 3 Â 10 7 CFU (Hines et al, 2007) per mg pelleted wet weight and averaging approximately 1 Â 10 7 CFU/mg (Sweeney and Hines, unpublished information). A retrospective estimate of the number of CFU inoculated should be made by performing colony counts plated on serial dilutions.…”

Section: Methods Of Quantifying Inoculummentioning

confidence: 99%

See 1 more Smart Citation

Experimental challenge models for Johne's disease: A review and proposed international guidelines

Hines

Stabel

Sweeney

et al. 2007

Veterinary Microbiology

Self Cite

125

140

View full text Add to dashboard Cite

An international committee of Johne's disease (JD) researchers was convened to develop guidelines for JD challenge studies in multiple animal species. The intent was to develop and propose international standard guidelines for models based on animal species that would gain acceptance worldwide. Parameters essential for the development of long-term and short-term infection models were outlined and harmonized to provide a ''best fit'' JD challenge model for cattle, goats, sheep, cervids, and mice. These models will be useful to study host-pathogen interactions, host immunity at the local and systemic level, and for evaluating vaccine candidates and therapeutics. The consensus guidelines herein list by animal species strains of Mycobacterium avium subsp. paratuberculosis used, challenge dose, dose frequency, age of challenge, route of challenge, preparation of inoculum, experimental animal selection, quality control, minimal experimental endpoints and other parameters. #

show abstract

Section: Methods Of Quantifying Inoculummentioning

confidence: 99%

“…To standardize experiments between laboratories, propagation of MAP in-vitro from a master seedstock of the chosen strain in mid-log phase growth should be employed. Middlebrook 7H9 broth, supplemented with OADC, mycobactin J, and 1% glycerol is recommended for the propagation of inoculum (Hines et al, 2007).…”

Section: Long-term Bovine Map Challenge Modelmentioning

confidence: 99%

Experimental challenge models for Johne's disease: A review and proposed international guidelines

Hines

Stabel

Sweeney

et al. 2007

Veterinary Microbiology

Self Cite

125

140

View full text Add to dashboard Cite

show abstract

“…Mice are cost effective, present some histological and immunological features similar to those of ruminants, and can serve as a preliminary screening model for vaccine candidates (39,41), but they do not develop disease typical of that seen with ruminants with paratuberculosis (9,41). In comparison, goats are much more costly but provide a direct homologue of the disease in cattle and other ruminants, which is beneficial for pathogenesis and vaccine efficacy studies (21,32).…”

mentioning

confidence: 99%

Assessment of Live Candidate Vaccines for Paratuberculosis in Animal Models and Macrophages

et al. 2010

View full text Add to dashboard Cite

Mycobacterium avium subsp. paratuberculosis (basonym M. paratuberculosis) is the causative agent of paratuberculosis, a chronic enteritis of ruminants. To control the considerable economic effect that paratuberculosis has on the livestock industry, a vaccine that induces protection with minimal side effects is required. We employed transposon mutagenesis and allelic exchange to develop three potential vaccine candidates, which were then tested for virulence with macrophages, mice, and goats. All three models identified the WAg906 mutant as being the most attenuated, but some differences in the levels of attenuation were evident among the models when testing the other strains. In a preliminary mouse vaccine experiment, limited protection was induced by WAg915, as evidenced by a reduced bacterial load in spleens and livers 12 weeks following intraperitoneal challenge with M. paratuberculosis K10. While we found macrophages and murine models to be rapid and cost-effective alternatives for the initial screening of M. paratuberculosis mutants for attenuation, it appears necessary to do the definitive assessment of attenuation with a ruminant model.

show abstract

“…Reagents and methods of the Johne's AGID protocol at the New York Animal Health Diagnostic Laboratory (NYAHDL) were used for AGID testing [10,11]. AGID was performed in 0.8% agarose gel prepared in phosphate buffered saline on petri plates (100mm x 15mm) using an immunodiffusion template to create a seven-well pattern, with a center well and six outside wells.…”

Section: Serological Assaysmentioning

confidence: 99%

“…In a previous JD vaccine efficacy study [11], some kids were vaccinated with JD vaccines, but were not challenged. Testing for MAP-antibodies was conducted using the same approved ELISA and AGID tests as used in this study.…”

Section: Evaluation Of a New Enzyme-linked Immunosorbent Assay (Elisamentioning

confidence: 99%

Evaluation of a New Enzyme-Linked Immunosorbent Assay (ELISA) for Johne's Disease in Goat Serum

White¹,

Marotti²,

Hines³

et al. 2016

SOJVS

View full text Add to dashboard Cite

SOJ Veterinary Sciences Open Access Research Articleinfection has been demonstrated for cattle [2] and is assumed to be the case for all ruminants. Cross species transmission is possible, including in wildlife. MAP is highly resistant in the environment [3].There are four phases of the disease. First, silent infection, which can be detected only by necropsy and tissue culture. Second, subclinical disease, in which only a small percentage of animals have detectable antibodies and most animals are fecalculture-negative with intermittent shedding of low numbers of MAP in feces in only a few animals. Third, clinical disease, often seen several years after infection. In cattle, clinical signs are of gradual weight loss, rough hair coat, and diarrhea and drop in AbstractControl of Johne's Disease (JD) is a high priority, it causes significant economic losses in the livestock industry. An efficacious vaccine for JD is not currently available and no satisfactory treatment exists, so control programs depend on diagnostic tests based on detection of the immune response generated after infection with Mycobacterium avium subsp. paratuberculosis (MAP)-the causative agent of JD-to identify and remove infected animals and management practices to interrupt transmission. Objectives:The objective of this study was to evaluate a new, commercially-available, Enzyme-Linked, Immunosorbent Assay (ELISA) that was developed for cattle, for use with goat serum. For comparison, a United States Department of Agriculture (USDA)-approved ELISA kit for goats, an Agar Gel Immunodiffusion (AGID) test and a Polymerase Chain Reaction (PCR) test to detect MAP in fecal samples were also evaluated.Methods: Infection status at necropsy was taken as the reference standard, determined by gross, histological and microbiological examination of tissue specimens. The serum and fecal samples were taken from goat kids that had participated in a previous vaccine efficacy study. Eighty goat kids were vaccinated either once subcutaneously at eight weeks with a commercial vaccine, or orally at eight and 10 weeks with one of five experimental oral vaccines or a sham-control oral vaccine. Kids were challenged orally with a bovine isolate of MAP, three weeks after the last vaccination.Results: None of the vaccines prevented infection. Samples taken from 10 to 13 months post-challenge were used to compare the assays. The specificity of all tests was 100%. At 13 months, the new ELISA, approved ELISA, AGID test and fecal PCR test had sensitivities of 55.7%, 52.9%, 24.3% and 87.1% respectively. At 11 months the sensitivities were 48.6%, 38.6%, 10.0% and 78.6% respectively and the sensitivity of the new ELISA was significantly better than the approved ELISA (p = 0.0196). Conclusion:The new ELISA effectively detected circulating MAP antibody in the serum of infected goats and the preliminary results in this study indicate the kit has potential for use in goat JD control programs. The results for the new ELISA in goat serum are comparable with those reported when the ELIS...

show abstract

Efficacy of spheroplastic and cell-wall competent vaccines for Mycobacterium avium subsp. paratuberculosis in experimentally-challenged baby goats

Cited by 39 publications

References 23 publications

Experimental challenge models for Johne's disease: A review and proposed international guidelines

Experimental challenge models for Johne's disease: A review and proposed international guidelines

Assessment of Live Candidate Vaccines for Paratuberculosis in Animal Models and Macrophages

Evaluation of a New Enzyme-Linked Immunosorbent Assay (ELISA) for Johne's Disease in Goat Serum

Contact Info

Product

Resources

About