Efficacy of Spirulina platensis in improvement of the reproductive performance and easing teratogenicity in hyperglycemic albino mice

Pankaj, Pranay Punj

doi:10.4103/0253-7613.161271

Cited by 12 publications

(10 citation statements)

References 25 publications

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“…While, gestation period length and kindling rate were similar in G2 and G3 than in G1, but the difference was not significant (Table 2). Similarly, several authors discovered positive impact of Spirulina platensis on reproductive parameters in lambs (ElSabagh et al, 2014), poultry (Shanmugapriya et al, 2015) and mice (Pankaj, 2015). The positive effect of Spirulina platensis on reproductive measurements proved in the current study could be attributed to strong natural antioxidant (Abdel-Daim et al, 2015).…”

Section: Methodssupporting

confidence: 77%

Reproductive Performance, Oxidative Status and Blood Metabolites of Doe Rabbits Administrated With Spirulina Alga

2017

EPSJ

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ABSTRACT:The current study aimed to evaluate effects of oral administration with different levels of Spirulina platensis on reproductive performance, hematological and biochemical criteria, antioxidant activity, and liver and kidney histogenesis of APRI doe rabbits. A total of 45 nili-parous does at 16-18 wk of age was allotted to 3 groups (15 does / group). Does in the 1 st , 2 nd and 3 rd groups were daily received oral 3 ml distilled water containing 0 (G1) as control group, 300 (G2) and 600 (G3) mg/doe from Spirulina platensis for four weeks as a treatment period, respectively. At end of treatment, does were naturally mated by15 untreated fertile APRI bucks (5 bucks / group). Body weight (BW) of does, as initial and final of treatment (16-18 and 20-22 wk of age, respectively) and at kindling (22-26 wk of age), and of bunnies at birth and weaning were recorded for one parity. Daily feed intake (DFI) of does was recorded during treatment period. Conception (CR) and kindling (KR) rates and litter size (LS, total and live) of does, and bunny viability rate (VR) at birth and weaning were calculated. Plasma concentration of estradiol 17-β (E2) and progesterone (P4) of does on day 15 post-mating, hematological parameters, plasma biochemical and oxidative capacity at the end of treatment, and hepatic and renal histology of does at the end of experiment were determined. Results showed that doe final BW was heavier (P<0.05) in G2 than in G1 and G3. Change in BW (absolute or relative to initial BW), DFI, CR and LS of does, and VR and BW at birth and weaning of kids increased (P<0.05) in G2 and G3 than in G1, but KR was similar. Plasma E2 and P4 was higher (P<0.05), while E2/P4 ratio was lower (P<0.05) in G2 and G3 than in G1. Only E2 was higher (P<0.05) in G2 than in G3. Values of hemoglobin, red blood cells hematocrit, neutrophils and eosinophils increased (P<0.05) in G2 and G3, while platelets and lymphocytes, monocytes and acidophils decreased (P<0.05) in G2 and G3 compared with G1. The white blood cells decreased (P<0.05) only in G2, while erthrocytic values were not affected. Plasma total proteins, albumin, globulin and high density lipoprotein increased (P<0.05) in G2 and G3, while total lipids, triglycerides, total cholesterol and low density lipoprotein, aspartate aminotransferase, alanine aminotransferase, acid phosphatase and alkaline phosphatase decreased (P<0.05) in G2 and G3 compared with G1. Blood total antioxidant capacity, glutathione S-transferase, superoxide dismutase, glutathione and glutathione peroxidase increased (P<0.05), while thiobarbituric acid-reactive substances decreased (P<0.05) in G2 and G3 than in G1. Hepatic and renal tissues of treated does were normal. In conclusion, orally administrated Spirulina platensis (300 mg/doe) as natural antioxidants was proved to improve reproductive performance, blood constituents, antioxidative status without adverse effects on liver and kidney functions.

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Section: Methodssupporting

confidence: 77%

Reproductive Performance, Oxidative Status and Blood Metabolites of Doe Rabbits Administrated With Spirulina Alga

2017

EPSJ

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“…These compounds were also shown to reveal teratogenicity in a Danio rerio embryo experimental model [17,18]. However, the in vivo rodent models did not reveal any adverse effect of Chlorella or Spirulina supplementation on pregnancy outcome [20,21]; the use of the latter in pregnant women was shown by one study to correlate with oligohydramnios [22][23][24]. All in all, this highlights that exploring the presence of PMAs in commercially available microalgal supplements for human consumption is urgently needed.…”

Section: Introductionmentioning

confidence: 95%

Polymethoxy-1-Alkenes Screening of Chlorella and Spirulina Food Supplements Coupled with In Vivo Toxicity Studies

Henao

Murphy

Falfushynska

et al. 2020

Toxins

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Selected species of cyanobacteria and green algae have been reported to produce lipophilic polymethoxy-1-alkenes (PMAs) which were shown to exhibit in vivo teratogenicity. Considering that information on PMAs in Arthospira sp. (known commercially as Spirulina) and Chlorella sp. cultivated for food supplement production was essentially lacking, the present study screened Chlorella (n = 10) and Spirulina (n = 13) food supplements registered in the European Union. Mass spectrometry analysis of column fractionated extracts was performed. None of the four variants previously reported in some cyanobacteria and green algae, nor any potentially related structures were detected in the studied samples. Since the isolated lipophilic fractions contained various compounds, they were further screened for in vivo teratogenicity in Danio rerio embryo, and for the potential to induce oxidative stress and genotoxicity in the liver and neurotoxicity in the brain of adult zebrafish. None of the tested food supplements had detectable levels of PMAs or any potentially related structures. No teratogenicity was revealed except for spinal curvature induced by fractions obtained from two Chlorella products. Selected fractions revealed cytotoxicity as indicated by an increased level of reactive oxygen species, catalase activity, lipid peroxidation and increased frequency of DNA strand breaks in hepatic tissue. The majority (60%) of Chlorella fractions induced an increase in cholinesterase activity in zebrafish brain homogenate while exposure to 61.5% of Spirulina fractions was associated with its decrease. The present study confirms that Chlorella and Spirulina food supplements are free of teratogenic PMAs, although the observed in vivo toxicities raise questions regarding the quality of selected products.

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“…It causes nephropathy, retinopathy, neuropathy and embryopathy 2 . It is also associated with an increased incidence of cardiovascular disease, reduced life expectancy, significant morbidity due to microvascular complications and diminished quality of life.…”

Section: Introduction: Diabetes Mellitus (Dm) Is a Devastating And LImentioning

confidence: 99%

“…Dietary restrictions, planned exercise and administration of oral glucose lowering agents are applied widely to control elevated blood glucose level. Moreover, herbal supplements and other alternative medicines are widely used to treat diabetic disorders 2 .…”

Section: Introduction: Diabetes Mellitus (Dm) Is a Devastating And LImentioning

confidence: 99%

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2016

IJPSR

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ABSTRACT:The present study evaluates the hypoglycemic and hypolipidemic effects of supplementation of cell suspension of Spirulina platensis (SP) in alloxan (AXN) induced hyperglycemic mice. Diabetes was induced by intra-peritoneal administration of AXN. Mice with blood glucose level above 200 mg/dl were divided into Group I (control), Group II (diabetic control), Group III (diabetic control mice fed with SP), and Group IV (control mice fed with SP). General physiology, fasting blood glucose level, oral glucose tolerance test, liver glycogen content and lipid profile were recorded in test subjects. In hyperglycemic mice, a significant (P < 0.05) fractional increase in food intake (41.13%), water consumption (190.56%), fasting blood glucose level (273.2%), total cholesterol (144.12%), triglyceride (124.58%), very low density lipoprotein (124.59%) and low density lipoprotein (265.4%) with concomitant decrease in body weight (25.78%), liver glycogen (10.57%) and high density lipoprotein (37.74%) when compared with control at the end of the experiment but recuperation in these parameters were observed (P < 0.05) when subjected to SP treatment. SP resulted in fractional increased in body weight, liver glycogen and high density lipoprotein by 33.08%, 46.36% and 47.78 respectively, and dropped off food intake and water consumption to 19.76% and 61.76% respectively while fasting blood glucose level, total cholesterol, triglyceride, very low density lipoprotein and low density lipoprotein went down to 58.55%, 49.74%, 47.68%, 47.68% and 60.02% respectively. These findings validate that SP maintains general physiological status, glucose metabolism and lipid profile of diabetic mice and hence warrants further detailed dose-dependent studies to understand its mechanism of action.

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Efficacy of Spirulina platensis in improvement of the reproductive performance and easing teratogenicity in hyperglycemic albino mice

Cited by 12 publications

References 25 publications

Reproductive Performance, Oxidative Status and Blood Metabolites of Doe Rabbits Administrated With Spirulina Alga

Reproductive Performance, Oxidative Status and Blood Metabolites of Doe Rabbits Administrated With Spirulina Alga

Polymethoxy-1-Alkenes Screening of Chlorella and Spirulina Food Supplements Coupled with In Vivo Toxicity Studies

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