Efficacy of Supplemental Diet Gels for Preventing Postoperative Weight Loss in Mice (<i> Mus musculus</i>)

Gates, Katherine V.; Alamaw, Eden D; Jampachaisri, Katechan; Huss, Monika K; Pacharinsak, Cholawat

doi:10.30802/aalas-jaalas22-000030

Cited by 3 publications

(2 citation statements)

References 26 publications

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“…In developing this model, perioperative soft diet (DietGel, ClearH2O, 77-08-5022, Supplemental Table S1) was found to reduce the incidence of colonic obstruction that occurred if mice were continuously fed pelleted food and maintained body weight. 72 Thus, all mice were transitioned to DietGel 5 days prior to surgery and were maintained on it until necropsy. Compliance with established guidelines for humane use and care of laboratory animals was carried out as approved by the University of Minnesota Institutional Animal Care and Use Committee.…”

Section: Methodsmentioning

confidence: 99%

Diet-induced shifts in the gut microbiota influence anastomotic healing in a murine model of colonic surgery

Boatman,

Kaiser,

Nalluri-Butz

et al. 2023

Gut Microbes

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Host diet and gut microbiota interact to contribute to perioperative complications, including anastomotic leak (AL). Using a murine surgical model of colonic anastomosis, we investigated how diet and fecal microbial transplantation (FMT) impacted the intestinal microbiota and if a predictive signature for AL could be determined. We hypothesized that a Western diet (WD) would impact gut microbial composition and that the resulting dysbiosis would correlate with increased rates of AL, while FMT from healthy, lean diet (LD) donors would reduce the risk of AL. Furthermore, we predicted that surgical outcomes would allow for the development of a microbial preclinical translational tool to identify AL. Here, we show that AL is associated with a dysbiotic microbial community characterized by increased levels of Bacteroides and Akkermansia . We identified several key taxa that were associated with leak formation, and developed an index based on the ratio of bacteria associated with the absence and presence of leak. We also highlight a modifiable connection between diet, microbiota, and anastomotic healing, potentially paving the way for perioperative modulation by microbiota-targeted therapeutics to reduce AL.

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Section: Methodsmentioning

confidence: 99%

Diet-induced shifts in the gut microbiota influence anastomotic healing in a murine model of colonic surgery

Boatman,

Kaiser,

Nalluri-Butz

et al. 2023

Gut Microbes

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“…In addition, supplementing with a diet gel decreases post-operative weight loss. You can start offering the diet gel in addition to the normal diet a few days before the procedure to reduce neophobia (Gates et al, 2023).…”

Section: Of 16mentioning

confidence: 99%

Mouse Anesthesia and Analgesia

Schwartz,

Pacharinsak

2024

Current Protocols

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Providing anesthesia and analgesia for mouse subjects is a common and critical practice in the laboratory setting. This practice is necessary for performing invasive procedures, achieving prolonged immobility for sensitive imaging modalities (magnetic resonance imaging, for instance), and providing intra‐ and post‐procedural pain relief. In addition to facilitating the procedures performed by the investigator, the provision of anesthesia and analgesia is crucial for the preservation of animal welfare and for humane treatment of animals used in research. Furthermore, anesthesia and analgesia are important components of animal use protocols reviewed by Institutional Animal Care and Use Committees, requiring careful consideration and planning for the particular animal model. In this article, we provide technical guidance for the investigator, covering the provision of anesthesia by two routes (injectable and inhalant), guidelines for monitoring anesthesia, current techniques for recognition of pain, considerations for administering preventative analgesia, and considerations for post‐operative care. © 2024 Wiley Periodicals LLC.Basic Protocol 1: Injectable anesthesiaBasic Protocol 2: Inhalant anesthesiaBasic Protocol 3: Assessing pain

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Subcutaneous Alfaxalone-Xylazine-Buprenorphine for Surgical Anesthesia and Echocardiographic Evaluation of Mice (Mus musculus)

Young,

Kelly,

Anderson

et al. 2024

Journal of the American Association for Laboratory Animal Science

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Alfaxalone is a commonly used injectable anesthetic in dogs and cats due to its minimal cardiovascular side effects. Data for its use in mice are limited and demonstrate strain- and sex-associated differences in dose-response relationships. We performed a dose-comparison study of alfaxalone-xylazine-buprenorphine (AXB) in Crl:CFW(SW) mice. Subcutaneous injection of 50 mg/kg alfaxalone-10 mg/kg xylazine-0.1 mg/kg buprenorphine HCl consistently achieved a surgical plane of anesthesia (loss of toe pinch) for 48.6 ± 4.7 and 60.8 ± 9.6 min in females and males, respectively. The same dose and route of AXB induced a surgical plane of anesthesia in C57Bl/6NCrl (females: 42.3 ± 11.2 min; males: 51.6 ± 12.3 min), NCr-Foxn1nu (females: 76.8 ± 32.5 min; males: 80.0 ± 1.2 min), and NOD.Cg-PrkdcSCIDIl2rgtm1Wjl/SzJCr (females: 56.0 ± 37.2 min and males: 61.2 ± 10.2 min) mice. We found no significant difference in the duration of the surgical plane of anesthesia between males and females within the mouse strains Crl:CFW(SW), C57Bl/6NCrl, NCr-Foxn1nu, and NOD.Cg-PrkdcSCIDIl2rgtm1Wjl/SzJCr. We next performed an echocardiography study (n = 5 per group) of Crl:CFW(SW) mice (n = 5 per group) to compare subcutaneous AXB anesthesia with that produced by intraperitoneal injection of 100 mg/kg ketamine and 10 mg/kg xylazine (KX). AXB induced significantly less bradycardia (295.4 ± 29 bpm) than KX (185.8 ± 38.9 bpm) did, with no significant differences in cardiac output, ejection fraction, end-diastolic volume, end-systolic volume, or fractional shortening. These results suggest that subcutaneous administration of AXB is a viable alternative to KX for inducing a surgical plane of anesthesia in Crl:CFW(SW), C57Bl/6NCrl, NCr-Foxn1nu, and NOD.Cg-PrkdcSCIDIl2rgtm1Wjl/SzJCr mice, regardless of sex. AXB may also be a better injectable anesthetic option as compared with KX for avoiding adverse cardiac effects in mice.

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Efficacy of Supplemental Diet Gels for Preventing Postoperative Weight Loss in Mice ( Mus musculus)

Cited by 3 publications

References 26 publications

Diet-induced shifts in the gut microbiota influence anastomotic healing in a murine model of colonic surgery

Diet-induced shifts in the gut microbiota influence anastomotic healing in a murine model of colonic surgery

Mouse Anesthesia and Analgesia

Subcutaneous Alfaxalone-Xylazine-Buprenorphine for Surgical Anesthesia and Echocardiographic Evaluation of Mice (Mus musculus)

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