Efficacy of the Molded Carbon Sorbent VNIITU-1 Used in Obstetric Practice

Долгих, Т. И.; Пьянова, Г.; Баринов, В. А.; Лихолобов, А.; Долгіх, Я В; Рябчикова, И.; Корнеев, В.

doi:10.15360/1813-9779-2015-4-60-71

Cited by 3 publications

(3 citation statements)

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“…While impacting the inflammatory process itself, this approach would allow for local detoxification and adsorption of microbiota‐released toxins and cellular detritus from the uterine cavity, suppressing the local pro‐inflammatory cascade 15 . These requirements are met by carbon sorbents, which have been widely used in medical practice in the recent years 9,10 . Medical products from carbon can increase the efficiency of traditionally used drugs and help reduce the medication burden on the organism without the risk of new strains of resistant pathogens 16 .…”

Section: Discussionmentioning

confidence: 99%

“…Selection of a sorbent with a proper porous structure made it possible to influence the selectivity of sorption of certain microorganisms and the toxins they release, and to predetermine the therapeutic effect of the sorbent 8 . In this regard, we developed a special molded sorbent—modified by polyvinylpyrrolidone (FSMP) 10 …”

Section: Introductionmentioning

confidence: 99%

“…8 In this regard, we developed a special molded sorbent-modified by polyvinylpyrrolidone (FSMP). 10 FSMP has high chemical purity (carbon composition ≥99.5%) and a smooth surface of pseudospherical granules, measuring 0.5-1.0 mm. The sorbent is characterized by high robustness of the granules, mesoporous structure with the pore size between 50 and 60 nm, high adsorption activity for a wide range of substances, and an almost complete lack of dust on the surface and inside.…”

Section: Introductionmentioning

confidence: 99%

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The effectiveness of using a molded sorbent: Modified polyvinylpyrrolidone for the combined treatment of chronic endometritis

Баринов

Tirskaya

Borisova

et al. 2021

Intl J Gynecology & Obste

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Objective To improve the management of patients with chronic endometritis (CE) by using a molded sorbent—modified by polyvinylpyrrolidone (FSMP). Methods This prospective study included 70 patients with CE divided into two groups: group 1 (n = 23) received traditional antibiotic therapy (from days 3 to 10 of the menstrual cycle); group 2 (n = 47), received antibiotics and FSMP was inserted from days 5 to 10. Results At the end of therapy, group 1 had massive growth of pathogenic microflora in 21.7%, moderate growth in 69.6%, and no growth in 8.7% of cases. In group 2, after combined therapy, massive growth was observed in 4.3%, moderate growth in 44.7%, and no growth in 51.0%. In group 2 after 5 days, serum levels of interleukin‐1β (IL‐1β) were 1.9 times, of IL‐6 were 7.0 times, and of IL‐8 and IL‐1 receptor antagonist were 1.3 times lower than in group 1. In uterine cavity aspirates, IL‐1β decreased around 4.8 times, IL‐6 by 11.8 times, IL‐8 by 3.2 times, tumor necrosis factor‐α by 3.9 times, and IL‐1 receptor antagonist by 2.1 times in comparison to group 1. Conclusion Combined therapy of FSMP with antibiotics is more effective in treating CE, because it contributes to the almost complete elimination of pathogens and toxins from the uterine cavity, blocking the local pro‐inflammatory cascade.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The effectiveness of using a molded sorbent: Modified polyvinylpyrrolidone for the combined treatment of chronic endometritis

Баринов

Tirskaya

Borisova

et al. 2021

Intl J Gynecology & Obste

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Prospects of the Use of New Carbon Sorbents with Antimicrobial Properties in the Intensive Care Medicine

Долгих

Лихолобов

Мороз

et al. 2019

Obŝaâ reanimatologiâ

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The purpose is to study antibacterial and antimycotic properties of produced granular carbon sorbents. Material and methods. A VNIITU-1 carbon sorbent was used as a starting material, which was modified with polyvinylpyrrolidone and lactic and glycolic acids oligomers. Microbiological bench tests were carried out to determine the antibacterial and antimycotic activity of these granulated carbon hemosorbents. Antibacterial properties of sorbents were studied in relation to pathogenic and conditionally pathogenic microorganisms: Staphylococcus aureus, Pseudomonas aeroginosa, Klebsiella pneumonia, Escherichia coli, Streptococcus agalactiae as well as their mixtures: mixture No. 1 — S. aureus and E. coli; mixture No. 2 — S. aureus and P. aeruginosa. Results. Presented data demonstrate that the modified VNIITU-1 exhibits significant antibacterial and antimycotic activity against most of the studied microorganisms compared to original sorbent. Conclusion. Modified sorbents as antimicrobial and detoxifying drugs may further be employed in treatment of obstetrical-gynecological and surgical diseases. The carbon sorbents are promising materials for medicine, as they expand the potential of sorption therapy in clinical practice.

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Antibacterial activity of granulated carbon sorbents

Долгих¹,

Пьянова²,

Долгих³

et al. 2017

ZHurnal «Patologicheskaia Fiziologiia I Eksperimental`naia Terapiia»

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Цель исследования - изучение антибактериальной активности гранулированных углеродных сорбентов по отношению к патогенной микрофлоре в условиях различной продолжительности контакта с сорбентом. Методика. Исследовали антибактериальные свойства гранулированного сорбента ВНИИТУ-1, обработанного гидромеханически, окисленного и стабилизированного до нормативных значений рН и модифицированного поли-N-винилпирролидоном (ПВП) ВНИИТУ-1 по отношению к патогенным микроорганизмам: Staphylococcus aureus, Pseudomonas aeroginosa, Klebsiella pneumonia, Escherichia coli, Streptococcus agalactiae, а также к их смесям. Все микроорганизмы представляли собой клинические штаммы, выделенные из ран пациентов. Предварительно тест-микроорганизмы были исследованы на чувствительность к современным антибиотикам. Опытным путем подбирали такие разведения и количество засеваемого материала, чтобы выросшие на чашке Петри колонии можно было сосчитать. Бактерии сорбировали из физиологического раствора с концентрацией микробных клеток 3 х 10 колониеобразующих единиц в 1 мл исследуемой пробы (КОЕ/мл). В пробирку типа Эппендорф вносили сорбент в количестве 0,5 мл, добавляли 1 мл микробной взвеси, встряхивали для удаления пузырьков воздуха и выдерживали в термостате в течение 1, 3, 6 и 24 ч. По истечении заданного времени контакта сорбента с патогенной микрофлорой отбирали надосадочную жидкость в объеме 100 мкл, засевали на стерильные агаровые пластины чашек Петри с питательной средой ГМФ-агар. Засеянные чашки Петри помещали в СО-инкубатор 15АС (Япония) вверх дном и инкубировали при температуре 37 ± 1°С в течение 24 ± 2 ч. При подсчете колоний, выросших на чашке Петри, учитывали только те чашки, на которых выросло более 300 изолированных колоний. Результаты. Гранулированный сорбент ВНИИТУ-1 обладает антибактериальным действием в отношении как грамположительных, так и грамотрицательных бактерий, а также их смеси. Сроки проявления антибактериального эффекта сорбента зависят от вида микроорганизмов и времени их контакта с образцом. Присутствие в составе сорбента модификатора ПВП усиливает его антибактериальные свойства. Полимермодифицированный образец обладает пролонгированным антибактериальным эффектом в отношении широкого спектра микроорганизмов (E. coli, St. aureus, P. aeruginosae и St. aureus) и смеси их культур. Заключение. Показана возможность повышения антибактериальных свойств гранулированных углеродных сорбентов по отношению к патогенной микрофлоре путем их модификации поли-N-винилпирролидоном. Дальнейшая разработка и применение углеродных сорбентов как средств сорбционной терапии является перспективным подходом к местному лечению гнойных ран. The purpose: Study the antibacterial activity of granulated carbon sorbents in relation to the pathogenic microflora under the conditions of different period of contact with sorbent. Methods. It has been investigated the antibacterial properties of VNIITU-1 granulated sorbent, which was hydromechanically treated, oxidized and stabilized up to pH standard parameters and VNIITU-1 modified poly-N-vinylpyrrolidone (PVP) against the pathogenic micro-organisms, including Staphylococcus aureus, Pseudomonas aeroginosa, Klebsiella pneumonia, Escherichia coli, Streptococcus agalactiae, and their mixes. All bacteria were clinical strains derived from the wounds of patients. Dilutions and amount of inoculated material were experimentally selected for further calculation of the grown colonies on the Petri dish. Bacteria were sorbed from the saline solution with bacteria concentration of 3 х 10 of the colony forming units in 1 ml of test sample (CFU/ ml). Eppendorf tube was added the sorbent 0.5 ml and bacterial suspension 1 ml; then the tube was shaken to remove the air bubbles and incubated for 1, 3, 6 and 24 hr. Upon the expiry of the target time for sorbent with pathogenic flora contact the supernatant fluid was collected in 100 ul and inoculated on the sterile agar plates of the Petri dishes with GMF agar nutrient medium. Inoculated Petri dishes were placed upside down in СО incubator 15AC (Japan) and incubated at 37 ± 1°C for 24 ± 2 hours. When counting the colonies growing on the Petri dish, they took into consideration only the dishes where more than 300 isolated colonies were grown. If more than 300 colonies were grown, «> 3x102 CFU/ml» was recorded in the protocol. Results. Granulated sorbent VNIITU-1 has an antibacterial effect against both gram-positive and gram-negative bacteria, and their mixes. Time period for manifestations of the sorbent antibacterial effect depends on the type of microorganisms and time of contact. PVP presence in sorbent composition enhances the antibacterial effect of the sorbent. Resin-modified sample has a prolonged antibacterial effect against a broad spectrum of microorganisms (E. coli, St. aureus, P. aeruginosae and St. aureus) and mixtures of their cultures. Conclusion. It has been determined the possibility for increasing of antibacterial properties of granulated carbon sorbents against the pathogenic microflora by their modification with poly-N-vinylpyrrolidone. Further development and application of carbon sorbents as the means for sorption therapy is a perspective approach for the local treatment of purulent wounds.

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Efficacy of the Molded Carbon Sorbent VNIITU-1 Used in Obstetric Practice

Cited by 3 publications

References 1 publication

The effectiveness of using a molded sorbent: Modified polyvinylpyrrolidone for the combined treatment of chronic endometritis

The effectiveness of using a molded sorbent: Modified polyvinylpyrrolidone for the combined treatment of chronic endometritis

Prospects of the Use of New Carbon Sorbents with Antimicrobial Properties in the Intensive Care Medicine

Antibacterial activity of granulated carbon sorbents

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