Efficacy of Trichoderma asperellum, T. harzianum, T. longibrachiatum and T. reesei against Sclerotium rolfsii

Abstract

This work was carried out to select and to evaluate efficacy of Trichoderma isolates to control sclerotium wilt (Sclerotium rolfsii) of common-bean (Phaseolus vulgaris). For the experiments were used two isolates of S. rolfsii UB 193 and UB 228. Sixty-five Trichoderma spp. isolates were tested and the following ones were selected in vitro for the in vivo tests: 5, 11, 12, 15, 102, 103, 127, 136, 137, 1525 (T. longibrachiatum), 1637 (T. reesei), 1642, 1643 (T. harzianum), 1649 (T. harzianum), 1700 (T. asperellum) and EST 5. The most promising isolates were identified by Sequencing of the internal transcribed spacer regions ITS1, ITS2, and the 5.8s rRNA genomic region, using the ITS5 and ITS4 primers and compared with sequences in the National Center for Biotechnology Information (NCBI) database. These selected isolates 1649 (T. harzianum), 1525 (T. longibrachiatum) and 1637 (T. reesei) were tested for evaluation of sclerotial germination inhibition under laboratory conditions, and to evaluate the effects of these on disease of bean plants under greenhouse conditions. The Trichoderma isolates 1649, 1525 and 1637 were more efficient in reducing sclerotial germination. In addition to 1649, 1525 and 1637, the isolates 5, 12, 102 and 1525 (T. longibrachiatum) significantly reduced de amount of diseased bean plants under greenhouse conditions.

Characterisation and antifungal activity of extracellular chitinase from a biocontrol fungus, Trichoderma asperellum PQ34

“…against root rot and foliar pathogens. Pacheco et al (2016) evaluated the efficacy of six Trichoderma isolates to control sclerotium wilt (S. rolfsii) of common bean (Phaseolus vulgaris) and found that three of them (T. hazianum, T. longibrachiatum and T. reesei) were more efficient in reducing sclerotial germination. Marques et al (2018) isolated two strains CEN1245 and CEN1274, both belonging to the species T. brevicompactum, exhibiting a broad spectrum against S. rolfsii, C. gloesporioides, and the other disease fungi.…”

Athelia (Sclerotium) rolfsii in Allium sativum: potential biocontrol agents and their effects on plant metabolites

“…The biocontrol of A. rolfsii has been studied using several microorganisms and crop types that are attacked by this pathogen, such as fruit, leguminous, tuberous, and herbaceous crops ( Table I). The fungi and bacteria that have been tested include fungi in the genus Trichoderma (de Sousa and Blum 2013, Isaias et al 2014, Rao et al 2015, Dania et al 2016, 2017, Pacheco et al 2016, Islam et al 2017, Pseudomonas bacteria (Saritha et al 2015, Vaja et al 2016, Kotasthane et al 2017, and bacteria in the genus Bacillus (Abou-Aly et al 2015, Kumar et al 2015, Suneeta et al 2016, Figueredo et al 2017.…”

Vermicompost in combination with Trichoderma asperellum isolate T13 as bioagent to control sclerotium rot disease on vegetable soybean seedlings

“…Even though augmentation in controlling Rhizoctonia solani disease has been reported from the use of vermicompost and T. harzianum in the growing media (Ersahin et al, 2009), there has been no report on this effect on sclerotium rot disease. Since several Trichoderma spp., such as T. asperellum (Chamswarng and Intanoo, 2002;Charoenrak and Chamswarng, 2016;Pacheco et al, 2016), T. harzianum (Ozbay and Newman, 2004;Pacheco et al, 2016;Kamel et al, 2020;Junsopa et al, 2021) and T. koningii (Trutmann and Keane, 1990;Woo et al, 2014;Pacheco et al, 2016;Kamel et al, 2020) have been examined as effective biocontrol agents against soilborne plant pathogens, T. asperellum could be used for soil amendment together with vermicompost to control sclerotium rot disease.…”