Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings

Ramos‐Espinoza, Fernando Carlos; Cueva-Quiroz, Victor Alexander; Yunis‐Aguinaga, Jefferson; Alvarez-Rubio, Norquis Caled; Mello, Nicoli Paganoti de; Moraes, Julieta Rodini Engrácia de

doi:10.1016/j.fsi.2020.07.051

Cited by 27 publications

(15 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Streptococcus agalactiae , a Gram‐positive coccus (round bacterium) (Ramos‐Espinoza et al., 2020), could combine with TLR13 and further activate over‐expression on pro‐inflammatory cytokine (Gao et al., 2021). It induced the outbreak of a serious disease and high mortalities in tilapia in recent years.…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

Sun

et al. 2022

Journal of Fish Diseases

View full text Add to dashboard Cite

The tumour necrosis factor superfamily (TNFSF) plays critical roles in tumour apoptosis, tissue morphogenesis and lineage determination. TNFSF10 (TRAIL or Apol-2) belongs to the tumour necrosis factor (TNF) cytokine family and induces rapid apoptosis in a wide variety of tumour cell lines upon binding to death-inducing signalling receptors. In this study, we identified TNFSF10 from Nile tilapia (Oreochromis niloticus) and found it was most closely related to Japanese pufferfish (Takifugu rubripes) TNFSF10. Amino acid identity between tilapia TNFSF10 and mandarin fish (Siniperca chuatsi) TRAIL was 69.2%. The highest expression of TNFSF10 mRNA was observed in the liver. In vitro studies showed that the mRNA expression of TNFSF10 was significantly stimulated by LPS in head kidney leucocytes, but remarkably inhibited by Poly I:C in spleen leucocytes. In vivo studies showed Streptococcus agalactiae infection significantly induced the mRNA expression of TNFSF10 in both the head kidney and spleen. The soluble recombinant protein Trx-TNFSF10 could induce cytotoxicity and apoptosis in HeLa cells with cycloheximide as a promoter. Taken together, these results in this study indicate that TNFSF10 may play important roles in the immune system of Nile tilapia.

show abstract

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

Sun

et al. 2022

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…S. agalactiae strain (serotype Ib, SA43) isolated from naturally infected Nile tilapia with clinical signs of streptococcosis in the northern region of Paraná State, Brazil, was used in this study (Salvador et al, 2005). The preparation of the S. agalactiae vaccine was described in previous studies (Ramos‐Espinoza, Cueva‐Quiroz, Yunis‐Aguinaga, Alvarez‐Rubio, et al, 2020; Ramos‐Espinoza, Cueva‐Quiroz, Yunis‐Aguinaga, & Moraes, 2020). Briefly, S. agalactiae colonies were grown in Brain Heart Infusion (BHI) broth and incubated in an incubator shaker (C24 Classic Series, New Brunswick Scientific, New Jersey, USA) at 150 rpm, 28°C, for 72 h. Bacterial suspension was treated with H 2 O 2 in order to obtain a final concentration of 5% and incubated at 4°C for 6 h. Then, the bacteria were centrifuged at 10,000 × g , for 10 min, at 4°C, the supernatant discarded and the pellet resuspended in sterile phosphate‐buffered saline (PBS).…”

Section: Methodsmentioning

confidence: 99%

“…Our research group compared the performance of a novel H 2 O 2 ‐inactivated vaccine against S. agalactiae in Nile tilapia with classical formaldehyde‐inactivated vaccine, showing equivalent efficacies (Ramos‐Espinoza, Cueva‐Quiroz, Yunis‐Aguinaga, & Moraes, 2020). In addition, the H 2 O 2 ‐inactivated vaccine showed optimal levels of protection with the inclusion of aluminum hydroxide (relative percent survival [RPS]: 59.3%) and Freund's Incomplete Adjuvant (FIA) (RPS: 77.8%) (Ramos‐Espinoza, Cueva‐Quiroz, Yunis‐Aguinaga, Alvarez‐Rubio, et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Effect of dietary inclusion of mannan oligosaccharides on the efficacy of a novel hydrogen peroxide‐inactivated vaccine against Streptococcus agalactiae in Nile tilapia

Ramos‐Espinoza,

Cueva‐Quiroz,

Alvarez‐Rubio

et al. 2023

J World Aquaculture Soc

Self Cite

View full text Add to dashboard Cite

Mannan oligosaccharides (MOS) have shown to stimulate immune response in different fish species, but the results may appear contradictory and have not been tested in conjunction with vaccination. We hypothesized that dietary MOS supplementation could enhance efficacy and immune responses after immunization with a novel hydrogen peroxide‐inactivated vaccine. Nile Tilapia, Oreochromis niloticus, fingerlings were distributed into four groups (supplemented or not, vaccinated or not) and were used to determine immune and hematological parameters and somatic indexes. For the bacterial challenge and vaccine efficacy test, fish were assigned to five experimental groups. Twenty‐eight days after the feeding trial and vaccination, the groups were challenged intraperitoneally and monitored for 21 days. The relative percent survival of the groups basal diet‐vaccinated and MOS diet‐vaccinated were 98.1% and 100.0%, respectively. The dietary inclusion of MOS after vaccination for 28 days had no influence on vaccine efficacy, which could be explained because of the high efficacy of the vaccine itself. Therefore, we suggest that the contribution of MOS supplementation to immune responses after vaccination in fish would be greater for vaccines with lower efficacy, or when tested for heterologous challenges or for crossed protection.

show abstract

“…Therefore, conjugation with a large immunogenic carrier called an adjuvant is crucial to permit a prolonged antigen release [62]. Different types of adjuvants have been tested for fish vaccines, such as alginate microspheres encapsulated [63], nanoparticle [64], Freund's incomplete adjuvant (FIA) [65], and polysaccharides [66], with promising results. The effect of oral vaccination of killed wholecell S. agalactiae vaccine on stimulating GALTs in tilapia was previously investigated by Firdaus-Nawi et al [67].…”

Section: Development Of a Vaccine Against S Agalactiaementioning

confidence: 99%

Current status and advances of fish vaccines in Malaysia

Ridzuan

Abdullah²,

Ramly³

et al. 2022

Vet World

View full text Add to dashboard Cite

Fish diseases have a significant negative influence on the Malaysian aquaculture industry. Since the 1980s, the sector has grown in size, which has resulted in a rise in the prevalence of infectious outbreaks affecting both freshwater and marine cultured fish species. Demand for commercially available fish vaccinations is predicted to increase as infectious disease outbreaks continue to occur. In Malaysia, aquaculture vaccine research and development (R&D) are still in its infancy, with most efforts concentrating on producing vaccines against bacterial infections, most notably streptococcosis, vibriosis, and motile Aeromonas septicemia. Despite several attempts, no homegrown vaccine has been effectively introduced into the manufacturing pipeline to date. At the moment, only three imported aquatic vaccines have received full permission, a far cry from the 314 and 60 vaccines licensed in the poultry and porcine industries, respectively. This review will describe recent findings regarding the development of aquaculture vaccines for certain fish species and diseases in Malaysia. In our opinion, R&D on fish vaccines is critical to the aquaculture industry's viability.

show abstract

Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings

Cited by 27 publications

References 40 publications

Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

Molecular cloning, expressional analyses and functional identification of TRAIL in tilapia, Oreochromis niloticus

Effect of dietary inclusion of mannan oligosaccharides on the efficacy of a novel hydrogen peroxide‐inactivated vaccine against Streptococcus agalactiae in Nile tilapia

Current status and advances of fish vaccines in Malaysia

Contact Info

Product

Resources

About