Efficacy of two breath condensers

Davidsson, Anette; Schmekel, Birgitta

doi:10.1002/jcla.20389

Cited by 9 publications

(10 citation statements)

References 24 publications

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“…Unfortunately, the irreproducibility of EBC sampling (often attributed to uncontrolled dilution of respiratory droplets in the collected EBC and poor standardization of both sampling equipment and sampling procedure) still remains the bottleneck of the method, regardless of the analytical technique used for final analysis. Only few studies attempted to standardize and compare the collection equipment , condenser material , or find suitable dilution markers that would allow for standardized sampling. Standardization of ventilatory patterns is also very important because nonuniform breathing may contribute significantly to different amounts of respiratory droplets evolved during each exhalation .…”

Section: Introductionmentioning

confidence: 99%

Single‐breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

2015

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The analysis of ionic content of exhaled breath condensate (EBC) from one single breath by CE with C(4) D is demonstrated for the first time. A miniature sampler made from a 2-mL syringe and an aluminum cooling cylinder for collection of EBC was developed. Various parameters of the sampler that influence its collection efficiency, repeatability, and effect of respiratory patterns were studied in detail. Efficient procedures for the cleanup of the miniature sampler were also developed and resulted in significant improvement of sampling repeatability. Analysis of EBC was performed by CE-C(4) D in a 60 mM MES/l-histidine BGE with 30 μM CTAB and 2 mM 18-crown-6 at pH 6 and excellent repeatability of migration times (RSD < 1.3% (n = 7)) and peak areas (RSD < 7% (n = 7)) of 12 inorganic anions, cations, and organic acids was obtained. It has been shown that the breathing pattern has a significant impact on the concentration of the analytes in the collected EBC. As the ventilatory pattern can be easily controlled during single exhalation, the developed collection system and method provides a highly reproducible and fast way of collecting EBC with applicability in point-of-care diagnostics.

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Section: Introductionmentioning

confidence: 99%

Single‐breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

2015

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“…Alternative breath collection devices may yield different quantities and qualities of EBC, although reliable data on this matter is disparate (Huttmann et al, 2011, Davidsson and Schmekel, 2010, Koczulla et al, 2009). Based on recent studies of our laboratory and others (Loyola et al, 2008, Czebe et al, 2008, Horvath et al, 2005, Liu et al, 2007), it is anticipated that conducting a similar clinical trial using continuously cooled EBC collection devices may yield more robust VOC data and further enhance the between-group differences.…”

Section: Discussionmentioning

confidence: 99%

A mobile instrumentation platform to distinguish airway disorders

et al. 2013

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Asthma and chronic obstructive pulmonary disease (COPD) are distinct but clinically overlapping airway disorders which often create diagnostic and therapeutic dilemmas. Current strategies to discriminate these diseases are limited by insensitivity and poor performance due to biologic variability. We tested the hypothesis that a gas chromatograph / differential mobility spectrometer (GC/DMS) sensor could distinguish between clinically well-defined groups with airway disorders based on the volatile organic compounds (VOCs) obtained from exhaled breath. After comparing VOC profiles obtained from 13 asthma, 5 COPD, and 13 healthy control subjects, we found that VOC profiles distinguished asthma from healthy controls and also a subgroup of asthmatics taking the drug omalizumab from healthy controls. The VOC profiles could not distinguish between COPD and any of the other groups. Our results show a potential application of the GC/DMS for non-invasive and bedside diagnostics of asthma and asthma therapy monitoring. Future studies will focus on larger sample sizes and patient cohorts.

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“…Dilution can come from multiple sources, including excess moisture in airway vapors, mucous formed from the airway epithelium, and in the case of mechanical ventilation, there is evidence that heated humidification systems may dilute EBC samples (3, 8). Along these lines, studies collecting EBC from mechanically ventilated patients have been performed with humidification connected (2, 9, 11, 16, 29, 37) and disconnected (12, 13, 14, 15, 16, 18). Müller et al studied the effects of humidification on EBC in mechanically ventilated children using 8-isoprostane as a biomarker.…”

Section: Considerations In Condensate Collectionmentioning

confidence: 99%

“…These include H 2 O 2 (12,19, 20, 22, 23), NO (11, 13, 14, 15), leukotrienes (12, 13), 8-isoprostane (3, 12, 13, 14, 15, 18), pH (2, 11, 12, 13, 14, 25), ammonia (25), cytokines (9, 11, 16, 25, 29), protein (29), MPO (12, 15, 37), and other specific receptors. Continuous pH monitoring has successfully been performed in mechanically ventilated patients (2).…”

Section: Analysis Of Ebc Samplesmentioning

confidence: 99%

Exhaled breath condensate collection in the mechanically ventilated patient

Carter

Davis

Kovacs

2012

Respiratory Medicine

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Collection of exhaled breath condensate (EBC) is a non-invasive means of sampling the airway-lining fluid of the lungs. EBC contains numerous measurable mediators, whose analysis could change the management of patients with certain pulmonary diseases. While initially popularized in investigations involving spontaneously breathing patients, an increasing number of studies have been performed using EBC in association with mechanical ventilation. Collection of EBC in mechanically ventilated patients follows basic principles of condensation, but is influenced by multiple factors. Effective collection requires selection of a collection device, adequate minute ventilation, low cooling temperatures, and sampling times of greater than ten minutes. Condensate can be contaminated by saliva, which needs to be filtered. Dilution of samples occurs secondary to distilled water in vapors and humidification in the ventilator circuit. Dilution factors may need to be employed when investigating non-volatile biomarkers. Storage and analysis should occur promptly at −70° C to −80° C to prevent rapid degradation of samples. The purpose of this review is to examine and describe methodologies and problems of EBC collection in mechanically ventilated patients. A straightforward and safe framework has been established to investigate disease processes in this population, yet technical aspects of EBC collection still exist that prevent clinical practicality of this technology. These include a lack of standardization of procedure and analysis of biomarkers, and of normal reference ranges for mediators in healthy individuals. Once these procedural aspects have been addressed, EBC could serve as a non-invasive alternative to invasive evaluation of lungs in mechanically ventilated patients.

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Efficacy of two breath condensers

Abstract: Variability in gain was large and ECoScreen was more efficacious then RTube in condensing the tested solutes of HNL, but not those of MPO or chlorine.

Cited by 9 publications

References 24 publications

Single‐breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

Single‐breath analysis using a novel simple sampler and capillary electrophoresis with contactless conductometric detection

A mobile instrumentation platform to distinguish airway disorders

Exhaled breath condensate collection in the mechanically ventilated patient

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