Efficacy of two fluorescence in situ hybridization (FISH) probes for diagnosing malignant pleural effusions

Rosolen, Debora; Kulikowski, Leslie Domenici; Bottura, G.; Nascimento, Amon M.; Acencio, Milena Marques Pagliarelli; Teixeira, Lisete R.; Vargas, Francisco S.; Sales, Roberto Moura; Antonângelo, Leila

doi:10.1016/j.lungcan.2013.02.001

Cited by 7 publications

(5 citation statements)

References 24 publications

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“…In a recent study, we used cells from fresh pleural fluid to demonstrate the usefulness of fluorescence in situ hybridization for the diagnosis of mpe, because the presence of aneuploid cells is commonly associated with malignancy 19 . Those results demonstrated the high sensitivity and specificity of fluorescence in situ hybridization for diagnosing mpe.…”

Section: Discussionmentioning

confidence: 99%

Pleural Fluid Tumour Markers in Malignant Pleural Effusion with Inconclusive Cytologic Results

et al. 2015

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Section: Discussionmentioning

confidence: 99%

Pleural Fluid Tumour Markers in Malignant Pleural Effusion with Inconclusive Cytologic Results

et al. 2015

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“…FISH was successfully applied to fresh effusions using protocols including fixation in Carnoy's solution (methanol: acetic acid, 3:1 ratio) to detect malignancy-associated aberrations in chromosomes 11 and 17 [45] or 7, 11 and 17 [46]. The same solution was applied in analysis of CDKN2A, the gene coding for p16 at chromosome 9p21, which is deleted in different cancers, including malignant mesothelioma [47,48].…”

Section: Other Ancillary Testingmentioning

confidence: 99%

Pre-analytical issues in effusion cytology

Michael

Davidson²

2016

Pleura and Peritoneum

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Effusions or body cavity fluids are amongst the most commonly submitted samples to the cytology laboratory. Knowledge of proper collection, storage, preservation and processing techniques is essential to ensure proper handling and successful analysis of the sample. This article describes how the effusions should be collected and proper conditions for submission. The different processing techniques to extract the cellular material and prepare slides satisfactory for microscopic evaluation are described such as direct smears, cytospins, liquid based preparations and cell blocks. The article further elaborates on handling the specimens for additional ancillary testing such as immunostaining and molecular tests, including predictive ones, as well as future research approaches.

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“…Because benign reactive mesothelium can present tetraploidy, cells with these characteristics were excluded from the analysis. This criterion was previously used by Rosolen et al [13] and Flores-Staino et al [17] in similar work. The slides were evaluated by two independent observers, and the results represent the average of their measurements.…”

Section: Molecular Cytogenetic Studymentioning

confidence: 97%

“…Aneuploidy is a common finding in neoplastic cells [11], and the demonstration of abnormal cell DNA content is considered indicative of malignancy. Fluorescence in situ hybridization (FISH) has been used in cavity fluids to detect aneuploidy in interphase cells, circumventing the need for cell culture, which could delay the turnaround time (TAT) to result [12,13].…”

Section: Original Researchmentioning

confidence: 99%

Performance of theUroVysion^®FISHassay for the diagnosis of malignant effusions using two cutoff strategies

Rosolen

Faria

et al. 2018

Cancer Medicine

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The cytological examination of cavity fluids has limited sensitivity in the diagnosis of malignancy. Aneuploidy, which is commonly observed in neoplastic cells, could potentially be used as an ancillary diagnostic tool. To evaluate the detection of aneuploid cells in cavitary effusion samples using the fluorescence in situ hybridization (FISH) assay UroVysion ® with some adaptations and two different cutoff strategies. Seventy samples of pleural or peritoneal fluid with positive (n = 40), negative (n = 15), or suspicious (n = 15) oncotic cytology were subjected to FISH assay with the multitarget UroVysion ® kit, which is composed of probes that hybridize to the centromeric region of chromosomes 3, 7, and 17 and to the locus 9p21. FISH performance was evaluated using two different cutoffs: (1) the manufacturer's cutoff (M‐FISH) and 2) a proposed cutoff (P‐FISH). Using M‐FISH, the diagnostic sensitivity was 57.1%, specificity 87.5%, and accuracy 60.0%; with P‐FISH, the sensitivity was 87.3%, specificity 71.4%, and accuracy 85.7%. When combined with cytology, the sensitivity, specificity, and accuracy were 88.0%, 83.3%, and 87.8%, respectively. Malignant cells presented a predominance of chromosomal gains. The UroVysion ® test using the P‐FISH cutoff was effective in demonstrating aneuploid cells in all malignant effusions, confirming the diagnosis of malignancy even in cases with suspicious cytology.

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Efficacy of two fluorescence in situ hybridization (FISH) probes for diagnosing malignant pleural effusions

Cited by 7 publications

References 24 publications

Pleural Fluid Tumour Markers in Malignant Pleural Effusion with Inconclusive Cytologic Results

Pleural Fluid Tumour Markers in Malignant Pleural Effusion with Inconclusive Cytologic Results

Pre-analytical issues in effusion cytology

Performance of theUroVysion^®FISHassay for the diagnosis of malignant effusions using two cutoff strategies

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Efficacy of two fluorescence in situ hybridization (FISH) probes for diagnosing malignant pleural effusions

Cited by 7 publications

References 24 publications

Pleural Fluid Tumour Markers in Malignant Pleural Effusion with Inconclusive Cytologic Results

Pleural Fluid Tumour Markers in Malignant Pleural Effusion with Inconclusive Cytologic Results

Pre-analytical issues in effusion cytology

Performance of theUroVysion®FISHassay for the diagnosis of malignant effusions using two cutoff strategies

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Performance of theUroVysion^®FISHassay for the diagnosis of malignant effusions using two cutoff strategies