Efficiencies of introduction of an oil-oxidizing Dietzia maris strain and stimulation of natural microbial communities in remediation of polluted soil

Плешакова, Е. В.; Dubrovskaya, Ekaterina; Turkovskaya, O. V.

doi:10.1134/s0003683808040091

Cited by 14 publications

(10 citation statements)

References 8 publications

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“…They have been reported as alkane degraders [6][7][8][9][10], but more importantly, their ability to degrade recalcitrant materials such as polycyclic aromatic compounds, including naphthalene, phenanthrene, benzoate, fluoranthene signifies their importance. In addition, it has been reported that they act as contributory factors to elimination of oil-related contaminations by other hydrocarbon-degrading organisms [11,12,5].…”

Section: Introductionmentioning

confidence: 99%

Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production

Kavynifard

Ebrahimipour

Ghasempour

2015

J. Basic Microbiol.

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The aim of this study was isolation and characterization of a crude oil degrader and biosurfactant-producing bacterium, along with optimization of conditions for crude oil degradation. Among 11 isolates, 5 were able to emulsify crude oil in Minimal Salt Medium (MSM) among which one isolate, named KA1, showed the highest potency for growth rate and biodegradation. The isolate was identified as Dietzia cinnamea KA1 using morphological and biochemical characteristics and 16S rRNA gene sequencing. The optimal conditions were 510 mM NaCl, pH 9.0, 35 °C, and minimal requirement of 46.5 mM NH4 Cl and 2.10 mM NaH2 PO4 . Gravimetric test and Gas chromatography-Mass spectroscopy technique (GC-MS) showed that Dietzia cinnamea KA1 was able to utilize and degrade 95.7% of the crude oil after 5 days, under the optimal conditions. The isolate was able to grow and produce biosurfactant when cultured in MSM supplemented with crude oil, glycerol or whey as the sole carbon sources, but bacterial growth was occurred using molasses with no biosurfactant production. This is the first report of biosurfactant production by D. cinnamea using crude oil, glycerol and whey and the first study to report a species of Dietzia degrading a wide range of hydrocarbons in a short time.

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Section: Introductionmentioning

confidence: 99%

Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production

Kavynifard

Ebrahimipour

Ghasempour

2015

J. Basic Microbiol.

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“…2006). Dietzia strains are recognized as ideal bacteria for enhancing HC‐degrading activities of the surrounding bacteria increasing the hydrogenase and catalase activities of other key oil‐degrading species and therefore speeding up the biodegradation process (Pleshakova et al. 2008) and for their long‐term survival in the environment even under dry, resource‐limited conditions (Quatrini et al.…”

Section: Introductionmentioning

confidence: 99%

“…Gram-positive Corynebacterineae bacteria, including Dietzia strains, dominated the in situ bioremediation process of the stranded fuel after the Prestige oil spill catastrophe ), and Dietzia bacteria are frequently related to environments polluted with HC (Brito et al 2006;Kleinsteuber et al 2006;von der Weid et al 2006). Dietzia strains are recognized as ideal bacteria for enhancing HC-degrading activities of the surrounding bacteria increasing the hydrogenase and catalase activities of other key oil-degrading species and therefore speeding up the biodegradation process (Pleshakova et al 2008) and for their long-term survival in the environment even under dry, resource-limited conditions (Quatrini et al 2008;Alonso-Gutierrez et al 2009;Radwan et al 2010). Bihari et al (2011) recently published the functional analysis of alkane degradation by Dietzia ssp.…”

Section: Introductionmentioning

confidence: 99%

Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)

Alonso-Gutierrez

Teramoto

Yamazoe

et al. 2011

Journal of Applied Microbiology

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Aims: Investigation of the alkane‐degrading properties of Dietzia sp. H0B, one of the isolated Corynebacterineae strains that became dominant after the Prestige oil spill. Methods and Results: Using molecular and chemical analyses, the alkane‐degrading properties of strain Dietzia sp. H0B were analysed. This Grampositive isolate was able to grow on n‐alkanes ranging from C12 to C38 and branched alkanes (pristane and phytane). 8‐Hexadecene was detected as an intermediate of hexadecane degradation by Dietzia H0B, suggesting a novel alkane‐degrading pathway in this strain. Three putative alkane hydroxylase genes (one alkB homologue and two CYP153 gene homologues of cytochrome P450 family) were PCR‐amplified from Dietzia H0B and differed from previously known hydroxylase genes, which might be related to the novel degrading activity observed on Dietzia H0B. The alkane degradation activity and the alkB and CYP153 gene expression were observed constitutively regardless of the presence of the substrate, suggesting additional, novel pathways for alkane degradation. Conclusions: The results from this study suggest novel alkane‐degrading pathways in Dietzia H0B and a genetic background coding for two different putative oil‐degrading enzymes, which is mostly unexplored and worth to be subject of further functional analysis. Significance and Impact of the Study: This study increases the scarce information available about the genetic background of alkane degradation in genus Dietzia and suggests new pathways and novel expression mechanisms of alkane degradation.

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“…In this case, the methods of monitoring and characterization of hydrocarbon degrading activity of soil microbiota are of special interest (Margesin and Schinner 2005;Abbassi and Shquirat 2008;Pleshakova et al 2008).…”

Section: Introductionmentioning

confidence: 98%

Microbial Actvity and¹³C/¹²C Ratio as Evidence of N-Hexadecane and N-Hexadecanoic Acid Biodegradation in Agricultural and Forest Soils

Zyakun

Nii-Annang

Franke

et al. 2011

Geomicrobiology Journal

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The dynamics of microbial degradation of exogenous contaminants, n-hexadecane and its primary microbial oxidized metabolite, n-hexadecanoic (palmitic) acid, was studied for topsoils, under agricultural management and beech forest on the basis the changes in O 2 uptake, CO 2 evolution and its associated microbial and non-microbial carbon isotopic signature, the respiratory quotient (RQ) and the priming effect (PE) of substrates. Soil microbial communities in agricultural soil responded to the n-hexadecane addition more rapidly compared to those of forest soil, with lagperiods of about 23 ± 10 and 68 ± 13 hours, respectively. Insignificant difference in the lag-period duration was detected for agricultural (t lag = 30 ± 13 h) and forest (t lag = 30 ± 14 h) soils treated with n-hexadecanoic (palmitic) acid. These results demonstrate that the soil microbiota differed in metabolic activities for using n-hexadecane as a reductive hydrocarbon and n-hexadecanoic acid as a partly oxidized hydrocarbon. The corresponding δ 13 C of respired CO 2 after the addition of the hydrocarbon contaminants to soils indicates a shift in microbial activity towards the consumption of exogenous substrates with a more complete degradation of n-hexadecane in the agricultural soil, for which some initial contents of hydrocarbons are inherent. It was reflected in the carbon isotope signature of microbial biomass. It is supposed that the observed deviation of RQ from theoretically calculated value under microbial substrate mineralization is determined by difference in the time ( t i ) of registration of CO 2 production and O 2 consumption. Positive priming effect (PE) of n-hexadecane and negative PE of n-hexadecanoic (palmitic) acid were detected in agricultural and forest soils. It is suggested that positive PE of n-hexadecane is conditioned by the induction of microbial enzymes that perform hydroxylation/oxygenation of stable SOM compounds mineralized by soil microbiota to CO 2 . The microbial metabolism coupled with oxidative decarboxylation of n-hexadecanoic acid is considered as one of the most probable causes of the revealed negative PE value.

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Efficiencies of introduction of an oil-oxidizing Dietzia maris strain and stimulation of natural microbial communities in remediation of polluted soil

Cited by 14 publications

References 8 publications

Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production

Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production

Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)

Microbial Actvity and¹³C/¹²C Ratio as Evidence of N-Hexadecane and N-Hexadecanoic Acid Biodegradation in Agricultural and Forest Soils

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Efficiencies of introduction of an oil-oxidizing Dietzia maris strain and stimulation of natural microbial communities in remediation of polluted soil

Cited by 14 publications

References 8 publications

Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production

Optimization of crude oil degradation by Dietzia cinnamea KA1, capable of biosurfactant production

Alkane-degrading properties of Dietzia sp. H0B, a key player in the Prestige oil spill biodegradation (NW Spain)

Microbial Actvity and13C/12C Ratio as Evidence of N-Hexadecane and N-Hexadecanoic Acid Biodegradation in Agricultural and Forest Soils

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Microbial Actvity and¹³C/¹²C Ratio as Evidence of N-Hexadecane and N-Hexadecanoic Acid Biodegradation in Agricultural and Forest Soils