Efficiencies of NF and RO Membranes on Pharmaceutical Removal and Membrane Fouling Effects

Phadunghus, Katanchalee; Wongrueng, Aunnop; Rakruam, Pharkphum; Wattanachira, Suraphong; Punyapalakul, Patiparn

doi:10.4186/ej.2017.21.3.101

Cited by 6 publications

(1 citation statement)

References 34 publications

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“…Generally, solid-phase adsorption and oxidation methods have been primarily used in physical and chemical SMX removal processes, respectively. Phadunghus et al found that the removal rate of SMX by nanofiltration and reverse osmosis membrane separation technology could reach 94–98% ( 16 ). Yazdanbakhsh et al used the Mn-WO 3 /LED photocatalytic system to degrade SMX in wastewater, with a removal rate of up to 85% in 3 h ( 17 ).…”

Section: Introductionmentioning

confidence: 99%

Metabolic engineering of Paracoccus denitrificans for dual degradation of sulfamethoxazole and ammonia nitrogen

Zhou,

Zhu,

Niu

et al. 2023

Microbiol Spectr

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Sulfamethoxazole (SMX), as one of the most widely used sulfonamide antibiotics, has been frequently detected in the aqueous environment, posing potential risks to the environment and human health. Although microbial degradation methods have been widely applied, some issues remain, including low degradation efficiency and poor environmental adaptability. In this regard, constructing efficient degrading bacteria by metabolic engineering is an ideal solution to these challenges. In this study, we used Paracoccus denitrificans DYTN-1, a superior nitrogen removal environment strain, as chassis to construct an SMX degradation pathway, obtaining a new bacteria for simultaneous degradation of SMX and removal of ammonia nitrogen. In doing this, we first identified and characterized four native promoters of P. denitrificans DYTN-1 with gradient strength to control the expression of the SMX degradation pathway. After degradation pathway expression level optimization and FMN reductase optimization, SMX degradation efficiency was significantly improved. The constructed P. d- pIAB 4 -P CS -sutR strain exhibited superior co-degradation of SMX and ammonia nitrogen contaminants with degradation rates of 44% and 71%, respectively. This study could pave the way for SMX degradation engineered strain design and evolution of environmental bioremediation. IMPORTANCE The abuse of sulfamethoxazole (SMX) had led to an increased accumulation in the environment, resulting in the disruption of the structure of microbial communities, further disrupting the bio-degradation process of other pollutants, such as ammonia nitrogen. To solve this challenge, we first identified and characterized four native promoters of Paracoccus denitrificans DYTN-1 with gradient strength to control the expression of the SMX degradation pathway. Then SMX degradation efficiency was significantly improved with degradation pathway expression level optimization and FMN reductase optimization. Finally, the superior nitrogen removal environment strain, P. denitrificans DYTN-1, obtained an SMX degradation function. This pioneering study of metabolic engineering to enhance the SMX degradation in microorganisms could pave the way for designing the engineered strains of SMX and nitrogen co-degradation and the environmental bioremediation.

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Section: Introductionmentioning

confidence: 99%